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    leuO DNA-binding transcriptional dual regulator LeuO [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 949034, updated on 17-Dec-2024

    Summary

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    Official Symbol
    leuO
    Official Full Name
    DNA-binding transcriptional dual regulator LeuO
    Primary source
    ECOCYC:EG10531
    Locus tag
    b0076
    See related
    ASAP:ABE-0000280
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK0078
    Summary
    LysR-family. leuO is upstream and divergent of the leuABCD operon. [More information is available at EcoGene: EG10531]. LeuO is a dual transcriptional regulator that regulates genes involved in leucine biosynthesis , genes involved in the utilization of certain beta-glucosides , and genes encoding LuxR-type transcription factors . [More information is available at EcoCyc: EG10531].
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    Genomic context

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    Sequence:
    NC_000913.3 (84368..85312)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene 3-isopropylmalate dehydrogenase Neighboring gene 2-isopropylmalate synthase Neighboring gene leu operon leader peptide Neighboring gene protein YabR Neighboring gene acetolactate synthase/acetohydroxybutanoate synthase, catalytic subunit

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Characterization of the pleiotropic LysR-type transcription regulator LeuO of Escherichia coli. Fragel SM, et al. Nucleic Acids Res, 2019 Aug 22. PMID 31184713, Free PMC Article
    2. Cloning of an EcoRI-generated fragment of the leucine operon of Salmonella typhimurium. Hertzberg KM, et al. Gene, 1980 Jan. PMID 6987127
    3. ppGpp-dependent leuO expression in bacteria under stress. Fang M, et al. Biochem Biophys Res Commun, 2000 Sep 16. PMID 11006083
    4. LeuO expression in response to starvation for branched-chain amino acids. Majumder A, et al. J Biol Chem, 2001 Jun 1. PMID 11376008
    5. LeuO-mediated transcriptional derepression. Chen CC, et al. J Biol Chem, 2003 Sep 26. PMID 12871947

    See all (30) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. DNA-binding transcriptional dual regulator LeuO (LeuO) is a tetramer that is formed by dimerization of the N-terminal DNA-binding domain (DBD) and C-terminal effector-binding domain (EBD).
      Title: Characterization of the pleiotropic LysR-type transcription regulator LeuO of Escherichia coli.
    2. BglJ-RcsB activates leuO, while LeuO inhibits activation by BglJ-RcsB. Furthermore, LeuO activates expression of bglJ, which is likewise H-NS repressed.
      Title: Correlation of Antagonistic Regulation of leuO Transcription with the Cellular Levels of BglJ-RcsB and LeuO in Escherichia coli.
    3. RcsB-BglJ binds to the leuO promoter region and activates one of two leuO promoters.
      Title: RcsB-BglJ activates the Escherichia coli leuO gene, encoding an H-NS antagonist and pleiotropic regulator of virulence determinants.
    4. LeuO is a major player in antagonistic interplay against the universal silencer H-NS.
      Title: Novel roles of LeuO in transcription regulation of E. coli genome: antagonistic interplay with the universal silencer H-NS.
    5. H-NS and LeuO are antagonistic regulators of CRISPR-based immunity.
      Title: H-NS-mediated repression of CRISPR-based immunity in Escherichia coli K12 can be relieved by the transcription activator LeuO.
    6. The results described herein indicate that the yjcRQP operon, one of the LeuO targets, is involved in sensitivity control against sulfa drugs.
      Title: Involvement of the leucine response transcription factor LeuO in regulation of the genes for sulfa drug efflux.
    7. LeuO and H-NS regulate the expression of the yjjQ-bglJ operon and yjjP.
      Title: Regulation of the yjjQ-bglJ operon, encoding LuxR-type transcription factors, and the divergent yjjP gene by H-NS and LeuO.
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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA-binding transcription factor activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables sequence-specific DNA binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in regulation of DNA-templated transcription IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in regulation of DNA-templated transcription IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within regulation of DNA-templated transcription IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    part_of protein-DNA complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    DNA-binding transcriptional dual regulator LeuO

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      84368..85312
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_414618.4 DNA-binding transcriptional dual regulator LeuO [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_414618.4

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A376DAV7
      Conserved Domains (1) summary
      PRK09508
      Location:1 → 314
      leuO; leucine transcriptional activator; Reviewed
    Nucleotide Protein
    Heading Accession and Version

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