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    nfsA NADPH-dependent nitro/quinone reductase NfsA [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 945483, updated on 3-Dec-2024

    Summary

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    Official Symbol
    nfsA
    Official Full Name
    NADPH-dependent nitro/quinone reductase NfsA
    Primary source
    ECOCYC:EG11261
    Locus tag
    b0851
    See related
    ASAP:ABE-0002900
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK0842; mda18; mdaA; ybjB
    Summary
    NfsA overexpression results in resistance to cytotoxic drugs includingAdriamycin and the tumoricidal agent DMP 840. nfsA is co-transcribed with ybjC. nfsA, nfsB, rutE, and ydjA are paralogs. [More information is available at EcoGene: EG11261]. The nfsA gene encodes the major oxygen-insensitive nitroreductase present in Escherichia coli. [More information is available at EcoCyc: EG11261].
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    Genomic context

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    Sequence:
    NC_000913.3 (891184..891906)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene putative inner membrane protein Neighboring gene reduced glutaredoxin 1 Neighboring gene DUF1418 domain-containing protein YbjC Neighboring gene ribosomal protein S6 modification protein Neighboring gene protein YbjN

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. The structures of E. coli NfsA bound to the antibiotic nitrofurantoin; to 1,4-benzoquinone and to FMN. Day MA, et al. Biochem J, 2021 Jul 16. PMID 34142705, Free PMC Article
    2. Conversion of NfsA, the major Escherichia coli nitroreductase, to a flavin reductase with an activity similar to that of Frp, a flavin reductase in Vibrio harveyi, by a single amino acid substitution. Zenno S, et al. J Bacteriol, 1998 Jan. PMID 9440535, Free PMC Article
    3. Crystallization and preliminary crystallographic analysis of major nitroreductase from Escherichia coli. Kobori T, et al. Acta Crystallogr D Biol Crystallogr, 1999 Nov. PMID 10531489
    4. Structure and site-directed mutagenesis of a flavoprotein from Escherichia coli that reduces nitrocompounds: alteration of pyridine nucleotide binding by a single amino acid substitution. Kobori T, et al. J Biol Chem, 2001 Jan 26. PMID 11034992
    5. Vibrio harveyi nitroreductase is also a chromate reductase. Kwak YH, et al. Appl Environ Microbiol, 2003 Aug. PMID 12902220, Free PMC Article

    See all (39) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Alterations in chromosomal genes nfsA, nfsB, and ribE are associated with nitrofurantoin resistance in Escherichia coli from the United Kingdom.
      Title: Alterations in chromosomal genes nfsA, nfsB, and ribE are associated with nitrofurantoin resistance in Escherichia coli from the United Kingdom.
    2. The structures of E. coli NfsA bound to the antibiotic nitrofurantoin; to 1,4-benzoquinone and to FMN.
      Title: The structures of E. coli NfsA bound to the antibiotic nitrofurantoin; to 1,4-benzoquinone and to FMN.
    3. Mutations in nfsA and nfsB were the main mechanisms leading to nitrofurantoin resistance, and overexpression of the oqxAB gene might help to further increase the minimum inhibitory concentrations of nitrofurantoin.
      Title: Unravelling mechanisms of nitrofurantoin resistance and epidemiological characteristics among Escherichia coli clinical isolates.
    4. reduction of quinones by NfsA is most consistent with a single-step (H(-)) hydride transfer mechanism
      Title: Reduction of quinones and nitroaromatic compounds by Escherichia coli nitroreductase A (NfsA): Characterization of kinetics and substrate specificity.
    5. Findings revealed that Phe42 is important for maintaining NfsA activity and structure.
      Title: Residue Phe42 is critical for the catalytic activity of Escherichia coli major nitroreductase NfsA.
    6. NfsA and NfsB, but not NemA, are responsible for the conversion of ethyl 2-(2'-nitrophenoxy)acetate.
      Title: Study of the role played by NfsA, NfsB nitroreductase and NemA flavin reductase from Escherichia coli in the conversion of ethyl 2-(2'-nitrophenoxy)acetate to 4-hydroxy-(2H)-1,4-benzoxazin-3(4H)-one (D-DIBOA), a benzohydroxamic acid with interesting biological properties.
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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables FMN binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables NAD(P)H dehydrogenase (quinone) activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables chromate reductase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables oxidoreductase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables protein homodimerization activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    NADPH-dependent nitro/quinone reductase NfsA
    NP_415372.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      891184..891906
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_415372.1 NADPH-dependent nitro/quinone reductase NfsA [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_415372.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A7A7AXF1
      Conserved Domains (1) summary
      PRK10765
      Location:1 → 240
      PRK10765; oxygen-insensitive NADPH nitroreductase
    Nucleotide Protein
    Heading Accession and Version

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