narX sensor histidine kinase NarX [Escherichia coli str. K-12 substr. MG1655] - Gene

Summary

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Official Symbol: narX
Official Full Name: sensor histidine kinase NarX
Primary source: ECOCYC:EG10646
Locus tag: b1222
See related: ASAP:ABE-0004099
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage: Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as: ECK1216; frd1; frdR; narR
Summary: The autophosphorylation of NarX is nitrate or nitrite dependent . [More information is available at EcoCyc: EG10646].
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Genomic context

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Sequence:: NC_000913.3 (1275822..1277618, complement)

NC_000913.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Results from bacterial adenylate cyclase two-hybrid analysis revealed robust interactions for the NarX-NarL, NarQ-NarL and NarQ-NarP pairs but a much weaker interaction for the NarX-NarP pair.
Title: Sensor-response regulator interactions in a cross-regulated signal transduction network.
Missense substitutions in NarX affect transmitter phosphatase function without directly changing the active-site region.
Title: Missense substitutions reflecting regulatory control of transmitter phosphatase activity in two-component signalling.
HAMP domain HD2 and S-helix regions overlap and form a continuous coiled-coil marked by a heptad repeat stutter. Impaired induction phenotypes were conferred by heptad deletions within the S-helix conserved core and that remove the heptad stutter.
Title: The S helix mediates signal transmission as a HAMP domain coiled-coil extension in the NarX nitrate sensor from Escherichia coli K-12.
study showed that only a small proportion of NarX and NarQ remain phosphorylated in the absence of nitrate, resulting in efficient response regulator dephosphorylation by the remaining unphosphorylated sensors
Title: Autophosphorylation and dephosphorylation by soluble forms of the nitrate-responsive sensors NarX and NarQ from Escherichia coli K-12.
analysis of FNR regulon and its interactions with nitrate, nitrite, NarXL, and NarQP
Title: A reassessment of the FNR regulon and transcriptomic analysis of the effects of nitrate, nitrite, NarXL, and NarQP as Escherichia coli K12 adapts from aerobic to anaerobic growth.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by EcoCyc

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables kinase activity	IEA Inferred from Electronic Annotation more info
enables phosphoprotein phosphatase activity	IDA Inferred from Direct Assay more info	PubMed
enables phosphorelay sensor kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables phosphorelay sensor kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables phosphorelay sensor kinase activity	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein dimerization activity	IEA Inferred from Electronic Annotation more info
enables protein homodimerization activity	IDA Inferred from Direct Assay more info	PubMed
enables protein kinase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within DNA damage response	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within cellular response to nitrate	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within cellular response to nitrite	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nitrate assimilation	IEA Inferred from Electronic Annotation more info
involved_in phosphorelay signal transduction system	IEA Inferred from Electronic Annotation more info
involved_in signal transduction	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within signal transduction	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in outer membrane-bounded periplasmic space	ISM Inferred from Sequence Model more info	PubMed
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	ISM Inferred from Sequence Model more info	PubMed

General protein information

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Preferred Names: sensor histidine kinase NarX

NP_415740.1

EC 2.7.13.3

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_000913.3 Reference assembly

Range

1275822..1277618 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NP_415740.1 sensor histidine kinase NarX [Escherichia coli str. K-12 substr. MG1655]

See identical proteins and their annotated locations for NP_415740.1

Status: PROVISIONAL

UniProtKB/TrEMBL

A0A5B9AN68

Conserved Domains (1) summary

PRK10600
Location:30 → 598

PRK10600; nitrate/nitrite two-component system sensor histidine kinase NarX