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    hybC hydrogenase 2 large subunit [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 945182, updated on 3-Dec-2024

    Summary

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    Official Symbol
    hybC
    Official Full Name
    hydrogenase 2 large subunit
    Primary source
    ECOCYC:EG11801
    Locus tag
    b2994
    See related
    ASAP:ABE-0009828
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK2988
    Summary
    HybC is co-exported bound to TAT substrate HybO. [More information is available at EcoGene: EG11801]. HybC is the large subunit of hydrogenase 2. [More information is available at EcoCyc: EG11801].
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    Genomic context

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    Sequence:
    NC_000913.3 (3141286..3142989, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene hydrogenase maturation factor HybG Neighboring gene hydrogenase maturation protein HybF Neighboring gene hydrogenase 2-specific chaperone Neighboring gene hydrogenase 2 maturation protease Neighboring gene hydrogenase 2 membrane subunit Neighboring gene hydrogenase 2 iron-sulfur protein Neighboring gene hydrogenase 2 small subunit

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Evidence for an oxygen-sensitive iron-sulfur cluster in an immature large subunit species of Escherichia coli [NiFe]-hydrogenase 2. Soboh B, et al. Biochem Biophys Res Commun, 2012 Jul 20. PMID 22735263
    2. Effect of alteration of the C-terminal extension on the maturation and folding of the large subunit of the Escherichia coli hydrogenase-2. Zhang M, et al. Biochimie, 2003 Jun. PMID 12829374
    3. Efficient electron transfer from hydrogen to benzyl viologen by the [NiFe]-hydrogenases of Escherichia coli is dependent on the coexpression of the iron-sulfur cluster-containing small subunit. Pinske C, et al. Arch Microbiol, 2011 Dec. PMID 21717143
    4. Isolation and characterisation of a soluble active fragment of hydrogenase isoenzyme 2 from the membranes of anaerobically grown Escherichia coli. Ballantine SP, et al. Eur J Biochem, 1986 Apr 15. PMID 3516690
    5. Transcriptional regulation in response to oxygen and nitrate of the operons encoding the [NiFe] hydrogenases 1 and 2 of Escherichia coli. Richard DJ, et al. Microbiology (Reading), 1999 Oct. PMID 10537212

    See all (25) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. a variant of the hydrogenase 2 large subunit, HybC, containing a fused C-terminal pentapeptide, was isolated.
      Title: Evidence for an oxygen-sensitive iron-sulfur cluster in an immature large subunit species of Escherichia coli [NiFe]-hydrogenase 2.
    2. Hydrogen-dependent reduction of benzyl viologen in hydrogenases HyaB, HybC and HybE is dependent on the [Fe-S]-cluster-containing small subunit.
      Title: Efficient electron transfer from hydrogen to benzyl viologen by the [NiFe]-hydrogenases of Escherichia coli is dependent on the coexpression of the iron-sulfur cluster-containing small subunit.
    3. Studies indicate that Hyd-1 and Hyd-2 are two H(2) uptake enzymes, while Hyd-1 is encoded by the first two genes of the hyaABCDEF operon, hyaA and hyaB, Hyd-2 is encoded by genes hybO and hybC of the hybOABCDEFG operon.
      Title: How Escherichia coli is equipped to oxidize hydrogen under different redox conditions.
    4. N-terminus verified by Edman degradation on mature peptide
      Title: Reassignment of the gene encoding the Escherichia coli hydrogenase 2 small subunit--identification of a soluble precursor of the small subunit in a hypB mutant.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables ferredoxin hydrogenase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables hydrogenase (acceptor) activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables nickel cation binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables nickel cation binding ISM
    Inferred from Sequence Model
    more info
    		 PubMed 
    enables oxidoreductase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in plasma membrane IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    hydrogenase 2 large subunit
    NP_417468.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      3141286..3142989 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_417468.1 hydrogenase 2 large subunit [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_417468.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A376M2H8, A0A6C9QER0, A0A8S7E9J3
      Conserved Domains (1) summary
      PRK10467
      Location:1 → 567
      PRK10467; hydrogenase 2 large subunit; Provisional
    Nucleotide Protein
    Heading Accession and Version

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