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    leuC 3-isopropylmalate dehydratase subunit LeuC [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 945076, updated on 3-Dec-2024

    Summary

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    Official Symbol
    leuC
    Official Full Name
    3-isopropylmalate dehydratase subunit LeuC
    Primary source
    ECOCYC:EG11576
    Locus tag
    b0072
    See related
    ASAP:ABE-0000261
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK0074
    Summary
    Isopropylmalate isomerase catalyzes the second step in leucine biosynthesis, converting 2-isopropylmalate to 3-isopropylmalate. [More information is available at EcoCyc: EG11576].
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    Genomic context

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    Sequence:
    NC_000913.3 (79464..80864, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene sugar exporter SetA Neighboring gene 3-isopropylmalate dehydratase subunit LeuD Neighboring gene 3-isopropylmalate dehydrogenase Neighboring gene 2-isopropylmalate synthase

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Heterologous expression and characterization of bacterial 2-C-methyl-D-erythritol-4-phosphate pathway in Saccharomyces cerevisiae. Carlsen S, et al. Appl Microbiol Biotechnol, 2013 Jul. PMID 23636690
    2. Salmonella typhimurium newD and Escherichia coli leuC genes code for a functional isopropylmalate isomerase in Salmonella typhimurium-Escherichia coli hybrids. Fultz PN, et al. J Bacteriol, 1979 Mar. PMID 374346, Free PMC Article
    3. Wild-type isopropylmalate isomerase in Salmonella typhimurium is composed of two different subunits. Fultz PN, et al. J Bacteriol, 1981 Oct. PMID 7026530, Free PMC Article
    4. Micromolar intracellular hydrogen peroxide disrupts metabolism by damaging iron-sulfur enzymes. Jang S, et al. J Biol Chem, 2007 Jan 12. PMID 17102132, Free PMC Article
    5. Aphidicolin inhibits DNA polymerase II of Escherichia coli, an alpha-like DNA polymerase. Chen H, et al. Nucleic Acids Res, 1990 Dec 11. PMID 2124684, Free PMC Article

    See all (28) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. copper treatment rapidly inactivated isopropylmalate dehydratase
      Title: The iron-sulfur clusters of dehydratases are primary intracellular targets of copper toxicity.
    2. N-terminus verified by Edman degradation on mature peptide
      Title: '98 Escherichia coli SWISS-2DPAGE database update.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables 3-isopropylmalate dehydratase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables 4 iron, 4 sulfur cluster binding IC
    Inferred by Curator
    more info
    		 PubMed 
    enables 4 iron, 4 sulfur cluster binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables lyase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in L-leucine biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within L-leucine biosynthetic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in L-leucine biosynthetic process NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in carboxylic acid metabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    part_of 3-isopropylmalate dehydratase complex EXP
    Inferred from Experiment
    more info
    		 PubMed 
    part_of 3-isopropylmalate dehydratase complex NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    3-isopropylmalate dehydratase subunit LeuC
    NP_414614.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      79464..80864 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_414614.1 3-isopropylmalate dehydratase subunit LeuC [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_414614.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A2H4TQM7, A0A8T6Q576
      Conserved Domains (1) summary
      PRK05478
      Location:1 → 466
      PRK05478; 3-isopropylmalate dehydratase large subunit
    Nucleotide Protein
    Heading Accession and Version

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