U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

Links from GEO Profiles

    • Showing Current items.

    metH cobalamin-dependent methionine synthase [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 948522, updated on 17-Dec-2024

    Summary

    Go to the top of the page Help
    Official Symbol
    metH
    Official Full Name
    cobalamin-dependent methionine synthase
    Primary source
    ECOCYC:EG10587
    Locus tag
    b4019
    See related
    ASAP:ABE-0013140
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK4011
    Summary
    [More information is available at EcoCyc: EG10587].
    NEW
    Try the new Gene table
    Try the new Transcript table

    Genomic context

    Go to the top of the page Help
    Sequence:
    NC_000913.3 (4223828..4227511)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene isocitrate dehydrogenase kinase/phosphatase Neighboring gene regulator of acetyl CoA synthetase Neighboring gene DNA-binding transcriptional repressor IclR Neighboring gene putative inorganic phosphate export protein YjbB Neighboring gene peptidase E

    Genomic regions, transcripts, and products

    Go to the top of the page Help
    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

    Go to the top of the pageHelp

    Related articles in PubMed

    1. Purification and assay of cobalamin-dependent methionine synthase from Escherichia coli. Jarrett JT, et al. Methods Enzymol, 1997. PMID 9250984
    2. Cobalamin-dependent methionine synthase from Escherichia coli B: electron paramagnetic resonance spectra of the inactive form and the active methylated form of the enzyme. Frasca V, et al. Biochemistry, 1988 Nov 1. PMID 2853966
    3. N5-methyltetrahydrofolate-homocysteine transmethylase. Role of S-adenosylmethionine in vitamin B12-dependent methionine synthesis. Taylor RT, et al. J Biol Chem, 1967 Apr 10. PMID 5337044
    4. Mutations affecting regulation of methionine biosynthetic genes isolated by use of met-lac fusions. Mulligan JT, et al. J Bacteriol, 1982 Aug. PMID 6284705, Free PMC Article
    5. Assignment of enzymatic function to specific protein regions of cobalamin-dependent methionine synthase from Escherichia coli. Drummond JT, et al. Biochemistry, 1993 Sep 14. PMID 8369297

    See all (46) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. describe an x-ray structure of the mutant fragment in the reactivation conformation
      Title: A disulfide-stabilized conformer of methionine synthase reveals an unexpected role for the histidine ligand of the cobalamin cofactor.
    2. N-terminus verified by Edman degradation on mature peptide
      Title: Cloning and sequence analysis of the Escherichia coli metH gene encoding cobalamin-dependent methionine synthase and isolation of a tryptic fragment containing the cobalamin-binding domain.
    Submit: New GeneRIF Correction

    Pathways from PubChem

    Go to the top of the page

    General gene information

    Go to the top of the page Help

    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables cobalamin binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables cobalamin binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables methionine synthase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables methionine synthase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables methionine synthase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables methyltransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables zinc ion binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables zinc ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in homocysteine metabolic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in homocysteine metabolic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within methionine biosynthetic process EXP
    Inferred from Experiment
    more info
    		 PubMed 
    involved_in methionine biosynthetic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in methionine biosynthetic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in methionine biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within methionine biosynthetic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in methylation IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in pteridine-containing compound metabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in tetrahydrofolate interconversion IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in tetrahydrofolate metabolic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    Component Evidence Code Pubs
    located_in cytoplasm IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in cytosol IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

    Go to the top of the page Help
    Preferred Names
    cobalamin-dependent methionine synthase
    NP_418443.1

    NCBI Reference Sequences (RefSeq)

    Go to the top of the page Help

    NEW Try the new Transcript table

    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      4223828..4227511
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_418443.1 cobalamin-dependent methionine synthase [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_418443.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A8T6PZC3
      Conserved Domains (1) summary
      PRK09490
      Location:1 → 1223
      metH; B12-dependent methionine synthase; Provisional
    Nucleotide Protein
    Heading Accession and Version

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.