narL DNA-binding transcriptional dual regulator NarL [Escherichia coli str. K-12 substr. MG1655] - Gene

Summary

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Official Symbol: narL
Official Full Name: DNA-binding transcriptional dual regulator NarL
Primary source: ECOCYC:EG10643
Locus tag: b1221
See related: ASAP:ABE-0004097
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage: Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as: ECK1215; frdR; narR
Summary: The protein belongs to the LuxR/UhpA family. [More information is available at EcoCyc: EG10643].
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Genomic context

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Sequence:: NC_000913.3 (1275179..1275829, complement)

NC_000913.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Structural basis for transcription activation by the nitrate-responsive regulator NarL.
Title: Structural basis for transcription activation by the nitrate-responsive regulator NarL.
Acetylation of NarL K188 and K192 is involved in regulating Escherichia coli anaerobic nitrate respiration.
Title: Acetylation of NarL K188 and K192 is involved in regulating Escherichia coli anaerobic nitrate respiration.
Activation by NarL at the Escherichia coli ogt promoter.
Title: Activation by NarL at the Escherichia coli ogt promoter.
Results from bacterial adenylate cyclase two-hybrid analysis revealed robust interactions for the NarX-NarL, NarQ-NarL and NarQ-NarP pairs but a much weaker interaction for the NarX-NarP pair.
Title: Sensor-response regulator interactions in a cross-regulated signal transduction network.
The NarL receiver domain operates as an on-off switch to occlude or release the output domain in a nitrate-responsive manner.
Title: The Escherichia coli NarL receiver domain regulates transcription through promoter specific functions.
NarL and NarP carboxyl-terminal domains activate transcription from Nar class II control regions.
Title: Functional roles for the GerE-family carboxyl-terminal domains of nitrate response regulators NarL and NarP of Escherichia coli K-12.
DNase I footprinting studies identified Fis-binding sites that overlap the yeaR promoter NarL site at position -43.5, and the ogt promoter NarL site at position -78.5, and suggest that Fis represses both promoters by displacing NarL.
Title: Competition between NarL-dependent activation and Fis-dependent repression controls expression from the Escherichia coli yeaR and ogt promoters.
The authors show that the global transcription factor FNR is a class II activator at the ynfEFGHI promoter and that NarL represses activation by binding to a single target that overlaps the promoter -10 element.
Title: Activation and repression at the Escherichia coli ynfEFGHI operon promoter.
Analysis of the DNA sequence of the ydhY promoter region (PydhY) revealed the presence of four heptameric sequences similar to NarL/P binding sites
Title: Characterization of the Escherichia coli K-12 ydhYVWXUT operon: regulation by FNR, NarL and NarP.
DNA-bound dimers of phospho-NarL can participate in higher-order cooperative interac
Title: Substitutions at auxiliary operator O3 enhance repression by nitrate-responsive regulator NarL at synthetic lac control regions in Escherichia coli K-12.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by EcoCyc

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables DNA-binding transcription repressor activity	IPI Inferred from Physical Interaction more info	PubMed
enables identical protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables transcription cis-regulatory region binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in negative regulation of DNA-templated transcription	IEA Inferred from Electronic Annotation more info
involved_in nitrate assimilation	IEA Inferred from Electronic Annotation more info
involved_in phosphorelay signal transduction system	IEA Inferred from Electronic Annotation more info
involved_in regulation of DNA-templated transcription	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within regulation of DNA-templated transcription	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
part_of protein-DNA complex	IPI Inferred from Physical Interaction more info	PubMed

General protein information

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Preferred Names: DNA-binding transcriptional dual regulator NarL

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_000913.3 Reference assembly

Range

1275179..1275829 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NP_415739.1 DNA-binding transcriptional dual regulator NarL [Escherichia coli str. K-12 substr. MG1655]

See identical proteins and their annotated locations for NP_415739.1

Status: PROVISIONAL

UniProtKB/TrEMBL

A0A7A3Q3N8

Conserved Domains (1) summary

PRK10651
Location:1 → 216

PRK10651; transcriptional regulator NarL; Provisional