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    pth peptidyl-tRNA hydrolase [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 945765, updated on 3-Dec-2024

    Summary

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    Official Symbol
    pth
    Official Full Name
    peptidyl-tRNA hydrolase
    Primary source
    ECOCYC:EG10785
    Locus tag
    b1204
    See related
    ASAP:ABE-0004043
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK1192; rap
    Summary
    Histidine 20 acts as the catalytic base during hydrolysis. [More information is available at EcoGene: EG10785]. Peptidyl-tRNA hydrolase is essential for growth in Escherichia coli. lysV, a lysine acceptor tRNA, was found to be a multicopy suppressor of a temperature sensitive pth mutant, indicating that the pth mutant phenotype is due to starvation for Lys-tRNA(Lys) . [More information is available at EcoCyc: EG10785].
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    Genomic context

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    Sequence:
    NC_000913.3 (1257929..1258513, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene putative autotransporter adhesin YcgV Neighboring gene redox-responsive ATPase YchF Neighboring gene stress-induced protein Neighboring gene aerobic C4-dicarboxylate transporter DauA

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Peptidyl-tRNA. VII. Substrate specificity of peptidyl-tRNA hydrolase. De Groot N, et al. Biochim Biophys Acta, 1969 Aug 20. PMID 4898482
    2. The fate of the initiator tRNAs is sensitive to the critical balance between interacting proteins. Thanedar S, et al. J Biol Chem, 2000 Jul 7. PMID 10748005
    3. Molecular basis for the temperature sensitivity of Escherichia coli pth(Ts). Cruz-Vera LR, et al. J Bacteriol, 2000 Mar. PMID 10692356, Free PMC Article
    4. Inhibition of translation and cell growth by minigene expression. Tenson T, et al. J Bacteriol, 1999 Mar. PMID 10049395, Free PMC Article
    5. Inhibition of Escherichia coli protein synthesis by abortive translation of phage lambda minigenes. Ontiveros C, et al. J Mol Biol, 1997 Jun 6. PMID 9191062

    See all (47) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Study identifies the amino acid residues involved in the tRNA moiety recognition and also revealed the mechanism by which Pth accepts the diverse sequences of the elongator-tRNAs as substrate components.
      Title: Structural basis for the substrate recognition and catalysis of peptidyl-tRNA hydrolase.
    2. crystals of peptidyl-tRNA hydrolase belonged to the hexagonal space group P6(1), with unit-cell parameters a = b = 55.1, c = 413.1 A
      Title: Crystallization and preliminary X-ray analysis of peptidyl-tRNA hydrolase from Escherichia coli in complex with the acceptor-TΨC domain of tRNA.
    3. N-terminus verified by Edman degradation on complete protein
      Title: Peptidyl-tRNA hydrolase is involved in lambda inhibition of host protein synthesis.
    4. Possible mechanism for the suppression of thermosensitivity of pth(Ts) mutants through tRNALys overproduction.
      Title: Excess of charged tRNALys maintains low levels of peptidyl-tRNA hydrolase in pth(Ts) mutants at a non-permissive temperature.
    Submit: New GeneRIF Correction

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables aminoacyl-tRNA hydrolase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables aminoacyl-tRNA hydrolase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables aminoacyl-tRNA hydrolase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables hydrolase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    acts_upstream_of_or_within protein quality control for misfolded or incompletely synthesized proteins IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within protein quality control for misfolded or incompletely synthesized proteins IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in translation IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    peptidyl-tRNA hydrolase
    NP_415722.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      1257929..1258513 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_415722.1 peptidyl-tRNA hydrolase [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_415722.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A232MCH0
      Conserved Domains (1) summary
      COG0193
      Location:2 → 192
      Pth; Peptidyl-tRNA hydrolase [Translation, ribosomal structure and biogenesis]
    Nucleotide Protein
    Heading Accession and Version

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