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    rho transcription termination factor Rho [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 948297, updated on 3-Dec-2024

    Summary

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    Official Symbol
    rho
    Official Full Name
    transcription termination factor Rho
    Primary source
    ECOCYC:EG10845
    Locus tag
    b3783
    See related
    ASAP:ABE-0012362
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK3775; nitA; nusD; psu; psuA; rnsC; sbaA; sun; tsu
    Summary
    rho has been shown to be an essential gene (Das, 1976; Bubunenko, 2007). [More information is available at EcoGene: EG10845]. Rho is required for one of the two major types of termination of RNA transcription. [More information is available at EcoCyc: EG10845].
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    Genomic context

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    Sequence:
    NC_000913.3 (3966417..3967676)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene thioredoxin 1 Neighboring gene rho operon leader peptide Neighboring gene UDP-N-acetylglucosamine--undecaprenyl-phosphate N-acetylglucosaminephosphotransferase Neighboring gene enterobacterial common antigen polysaccharide co-polymerase

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Analysis of E. coli rho factor: mutations affecting secondary-site interactions. Pereira S, et al. J Mol Biol, 1995 Aug 4. PMID 7643387
    2. Rho-dependent termination of transcription is governed primarily by the upstream Rho utilization (rut) sequences of a terminator. Richardson LV, et al. J Biol Chem, 1996 Aug 30. PMID 8702947
    3. Mutational analysis and secondary structure model of the RNP1-like sequence motif of transcription termination factor Rho. Martinez A, et al. J Mol Biol, 1996 Apr 19. PMID 8632473
    4. Structural organization of transcription termination factor Rho. Richardson JP. J Biol Chem, 1996 Jan 19. PMID 8576105
    5. A mutation in the ATP binding domain of rho alters its RNA binding properties and uncouples ATP hydrolysis from helicase activity. Pereira S, et al. J Biol Chem, 1995 Dec 22. PMID 8530466

    See all (219) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Transcriptional pause extension benefits the stand-by rather than catch-up Rho-dependent termination.
      Title: Transcriptional pause extension benefits the stand-by rather than catch-up Rho-dependent termination.
    2. Structural basis of Rho-dependent transcription termination.
      Title: Structural basis of Rho-dependent transcription termination.
    3. Crystal structure of the Escherichia coli transcription termination factor Rho.
      Title: Crystal structure of the Escherichia coli transcription termination factor Rho.
    4. Steps toward translocation-independent RNA polymerase inactivation by terminator ATPase rho.
      Title: Steps toward translocation-independent RNA polymerase inactivation by terminator ATPase ρ.
    5. Pre-termination Transcription Complex: Structure and Function.
      Title: Pre-termination Transcription Complex: Structure and Function.
    6. Data indicate transcription termination factor Rho (Rho) activity and predict Rho-dependent terminators in bacterial genomes.
      Title: A multivariate prediction model for Rho-dependent termination of transcription.
    7. Here we show that RNase H-deficient mutants of Escherichia coli exhibit heightened sensitivity to the Rho inhibitor bicyclomycin, and that Rho deficiency provokes increased formation of RNA-DNA hybrids (R-loops) which is ameliorated by expression of the phage T4-derived R-loop helicase UvsW.
      Title: Genome-wide relationship between R-loop formation and antisense transcription in Escherichia coli.
    8. Study conclude that Rho-dependent termination is capable of recycling RNA polymerases stalled at DNA lesions, thereby facilitating DNA repair and other DNA-dependent processes. Failure of this recycling leads to reduced survival of E.coli. Therefore Rho might either compete or augment the function of Mfd in vivo.
      Title: Rho-dependent transcription termination in bacteria recycles RNA polymerases stalled at DNA lesions.
    9. Our findings further show that the RNA sequence specificity used for guiding Rho-dependent termination derives in part from an intrinsic ability of the motor to couple the recognition of pyrimidine patterns in nascent transcripts to RNA loading and activity.
      Title: Ligand-induced and small-molecule control of substrate loading in a hexameric helicase.
    10. results, together with existing data, support a model in which the connector segment plays a hitherto overlooked role in the regulation of Rho-dependent termination
      Title: A Screen for rfaH Suppressors Reveals a Key Role for a Connector Region of Termination Factor Rho.
    Submit: New GeneRIF Correction See all GeneRIFs (32)

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ATP hydrolysis activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ATP-dependent activity, acting on RNA IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables RNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables RNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables helicase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables hydrolase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables identical protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    acts_upstream_of_or_within DNA-templated transcription termination IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in DNA-templated transcription termination IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cytosol IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in membrane HDA PubMed 

    General protein information

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    Preferred Names
    transcription termination factor Rho

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      3966417..3967676
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_418230.1 transcription termination factor Rho [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_418230.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A418GQZ0
      Conserved Domains (1) summary
      PRK09376
      Location:1 → 418
      rho; transcription termination factor Rho; Provisional
    Nucleotide Protein
    Heading Accession and Version

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