rpoE RNA polymerase sigma factor RpoE [Escherichia coli str. K-12 substr. MG1655] - Gene

Summary

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Official Symbol: rpoE
Official Full Name: RNA polymerase sigma factor RpoE
Primary source: ECOCYC:EG11897
Locus tag: b2573
See related: ASAP:ABE-0008467
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage: Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as: ECK2571; sigE
Summary: rpoE is transiently induced after a cold shock, dependent on the presence of PNPase. [More information is available at EcoGene: EG11897]. Sigma E is a minor sigma factor, specializing in responses to the effects of heat shock and other stresses on membrane and periplasmic proteins. [More information is available at EcoCyc: EG11897].
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Genomic context

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Sequence:: NC_000913.3 (2709437..2710012, complement)

NC_000913.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Interactions between DksA and Stress-Responsive Alternative Sigma Factors Control Inorganic Polyphosphate Accumulation in Escherichia coli.
Title: Interactions between DksA and Stress-Responsive Alternative Sigma Factors Control Inorganic Polyphosphate Accumulation in Escherichia coli.
The enhanced sigma E factor activity of DegS A323E substitution suppresses a number of assembly-defective conditions without exhibiting the toxicity associated with high levels of sigma E factor activity.
Title: Fine-Tuning of σ(E) Activation Suppresses Multiple Assembly-Defective Mutations in Escherichia coli.
Mapping of 5' ends of rpoE mRNA identified five new transcriptional initiation sites (P1 to P5) located distal to Esigma(E)-regulated promoter.
Title: Multiple Transcriptional Factors Regulate Transcription of the rpoE Gene in Escherichia coli under Different Growth Conditions and When the Lipopolysaccharide Biosynthesis Is Defective.
These results demonstrate that appropriate regulation of sigma(E) activity, ensuring that it is neither too high nor too low, is critical for envelope integrity and cell viability.
Title: Appropriate Regulation of the σ(E)-Dependent Envelope Stress Response Is Necessary To Maintain Cell Envelope Integrity and Stationary-Phase Survival in Escherichia coli.
This paper describes a unique mutation that enhances kinetics of the rpoE envelope stress response pathway rather than preinducing the response.
Title: A Suppressor Mutation That Creates a Faster and More Robust σE Envelope Stress Response.
In particular, Esigma(S) appears to contribute significantly to transcription of genes encoding proteins involved in LPS biosynthesis and in cell surface composition. Finally, our results highlight a direct role of Esigma(S) in the regulation of non coding RNAs
Title: Characterization of the Escherichia coli σ(S) core regulon by Chromatin Immunoprecipitation-sequencing (ChIP-seq) analysis.
RpoE regulates transcription of the defective prophage DLP12 lysis genes through interaction with QDLP12.
Title: The lysis cassette of DLP12 defective prophage is regulated by RpoE.
study found ppGpp is required for increased sigma(E) activity under all starvation conditions tested, but the requirement for DksA varies; DksA is required during amino acid starvation, but is dispensable during phosphate starvation
Title: Co-ordinated regulation of the extracytoplasmic stress factor, sigmaE, with other Escherichia coli sigma factors by (p)ppGpp and DksA may be achieved by specific regulation of individual holoenzymes.
However, membrane protein damage induced by gentamicin can be eliminated or repaired by RpoE- and Cpx-dependent mechanisms pre-emptively induced in P-starved cells.
Title: Protective role of the RpoE (σE) and Cpx envelope stress responses against gentamicin killing of nongrowing Escherichia coli incubated under aerobic, phosphate starvation conditions.
TraR increases the concentrations of periplasmic chaperones and proteases by directly activating the transcription of sigma(E)-dependent promoters.
Title: Activation of the σE-dependent stress pathway by conjugative TraR may anticipate conjugational stress.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by EcoCyc

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables sigma factor activity	IBA Inferred from Biological aspect of Ancestor more info
enables sigma factor activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in DNA-templated transcription	IEA Inferred from Electronic Annotation more info
involved_in DNA-templated transcription initiation	IDA Inferred from Direct Assay more info	PubMed
involved_in DNA-templated transcription initiation	IEA Inferred from Electronic Annotation more info
involved_in cellular response to cell envelope stress	NAS Non-traceable Author Statement more info	PubMed
involved_in negative regulation of DNA-templated transcription	NAS Non-traceable Author Statement more info	PubMed
involved_in regulation of DNA-templated transcription	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within regulation of DNA-templated transcription	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of DNA-templated transcription	IEA Inferred from Electronic Annotation more info
involved_in regulation of DNA-templated transcription initiation	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of DNA-templated transcription initiation	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within response to osmotic stress	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within response to temperature stimulus	IDA Inferred from Direct Assay more info	PubMed
involved_in submerged biofilm formation	NAS Non-traceable Author Statement more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
part_of cytosolic DNA-directed RNA polymerase complex	IPI Inferred from Physical Interaction more info	PubMed
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
part_of sigma factor antagonist complex	IPI Inferred from Physical Interaction more info	PubMed

General protein information

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Preferred Names: RNA polymerase sigma factor RpoE

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_000913.3 Reference assembly

Range

2709437..2710012 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NP_417068.1 RNA polymerase sigma factor RpoE [Escherichia coli str. K-12 substr. MG1655]

See identical proteins and their annotated locations for NP_417068.1

Status: PROVISIONAL

UniProtKB/TrEMBL

A0A5B9AQJ7

Conserved Domains (1) summary

TIGR02939
Location:1 → 189

RpoE_Sigma70; RNA polymerase sigma factor RpoE