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    thiE thiamine phosphate synthase [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 948491, updated on 17-Dec-2024

    Summary

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    Official Symbol
    thiE
    Official Full Name
    thiamine phosphate synthase
    Primary source
    ECOCYC:EG11586
    Locus tag
    b3993
    See related
    ASAP:ABE-0013055
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK3985; thiA
    Summary
    The ThiE sequence has been confirmed using MS/MS: the measured mass of the intact protein is 23014.8 indicating that the initial Met is not clipped (Kelleher, 1998). [More information is available at EcoGene: EG11586]. Thiamine phosphate synthase combines 4-methyl-5-(-hydroxyethyl)thiazole phosphate and 4-amino-5-hydroxymethyl-2-methylpyrimidine-pyrophosphate to generate thiamine phosphate . [More information is available at EcoCyc: EG11586].
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    Genomic context

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    Sequence:
    NC_000913.3 (4193569..4194204, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene 2-iminoacetate synthase Neighboring gene 1-deoxy-D-xylulose 5-phosphate:thiol sulfurtransferase Neighboring gene sulfur carrier protein ThiS Neighboring gene sulfur carrier protein ThiS adenylyltransferase Neighboring gene phosphomethylpyrimidine synthase Neighboring gene regulator of sigma D

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Purification and properties of thiaminephosphate pyrophosphorylase of Escherichia coli. Kayama Y, et al. Arch Biochem Biophys, 1973 Sep. PMID 4580841
    2. Thiamine phosphate pyrophosphorylase. Kawasaki T. Methods Enzymol, 1979. PMID 374983
    3. Efficient sequence analysis of the six gene products (7-74 kDa) from the Escherichia coli thiamin biosynthetic operon by tandem high-resolution mass spectrometry. Kelleher NL, et al. Protein Sci, 1998 Aug. PMID 10082377, Free PMC Article
    4. A genetic screen for the identification of thiamin metabolic genes. Lawhorn BG, et al. J Biol Chem, 2004 Oct 15. PMID 15292217
    5. The mechanism of action of bacimethrin, a naturally occurring thiamin antimetabolite. Reddick JJ, et al. Bioorg Med Chem Lett, 2001 Sep 3. PMID 11527707

    See all (12) citations in PubMed

    GeneRIFs: Gene References Into Functions

    Pathways from PubChem

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    General gene information

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    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables magnesium ion binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables magnesium ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metal ion binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables thiamine-phosphate diphosphorylase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables thiamine-phosphate diphosphorylase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables transferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in thiamine biosynthetic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within thiamine biosynthetic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in thiamine biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in thiamine diphosphate biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    is_active_in cytoplasm IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  

    General protein information

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    Preferred Names
    thiamine phosphate synthase
    NP_418421.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      4193569..4194204 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_418421.1 thiamine phosphate synthase [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_418421.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A1X3J9J8
      Conserved Domains (1) summary
      PRK03512
      Location:1 → 211
      PRK03512; thiamine phosphate synthase
    Nucleotide Protein
    Heading Accession and Version

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