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    thiM hydroxyethylthiazole kinase [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 945142, updated on 3-Dec-2024

    Summary

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    Official Symbol
    thiM
    Official Full Name
    hydroxyethylthiazole kinase
    Primary source
    ECOCYC:M007
    Locus tag
    b2104
    See related
    ASAP:ABE-0006960
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK2097
    Summary
    thiMD operon expression is controlled by a thiamine pyrophosphate (TPP) responsive riboswitch in the 5' UTR. [More information is available at EcoGene: EG14069]. Hydroxyethylthiazole kinase catalyzes one of two reactions that feed 4-methyl-5-(-hydroxyethyl)thiazole phosphate into the thiamine biosynthesis pathway. [More information is available at EcoCyc: M007].
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    Genomic context

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    Sequence:
    NC_000913.3 (2184513..2185301, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene putative sugar kinase YegV Neighboring gene putative DNA-binding transcriptional regulator YegW Neighboring gene putative glycosyl hydrolase YegX Neighboring gene bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase Neighboring gene DNA-binding transcriptional repressor RcnR Neighboring gene Ni(2(+))/Co(2(+)) exporter

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Purification and properties of 4-methyl-5-hydroxyethylthiazole kinase from Escherichia coli. Tani Y, et al. Biosci Biotechnol Biochem, 2016. PMID 26634770
    2. Molecular basis of gene regulation by the THI-box riboswitch. Ontiveros-Palacios N, et al. Mol Microbiol, 2008 Feb. PMID 18179415
    3. Ligand-induced folding of the thiM TPP riboswitch investigated by a structure-based fluorescence spectroscopic approach. Lang K, et al. Nucleic Acids Res, 2007. PMID 17693433, Free PMC Article
    4. Conformational changes in the expression domain of the Escherichia coli thiM riboswitch. Rentmeister A, et al. Nucleic Acids Res, 2007. PMID 17517779, Free PMC Article
    5. Crystal structures of the thi-box riboswitch bound to thiamine pyrophosphate analogs reveal adaptive RNA-small molecule recognition. Edwards TE, et al. Structure, 2006 Sep. PMID 16962976

    See all (21) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Structural analysis of ligand-induced folding of a thiamine pyrophosphate responsive riboswitch from Escherichia coli thiM mRNA.
      Title: Ligand-induced folding of the thiM TPP riboswitch investigated by a structure-based fluorescence spectroscopic approach.
    Submit: New GeneRIF Correction

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables catalytic activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables hydroxyethylthiazole kinase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables hydroxyethylthiazole kinase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables identical protein binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables kinase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables magnesium ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables prenol kinase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in thiamine biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in thiamine diphosphate biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within thiamine salvage IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    hydroxyethylthiazole kinase
    NP_416607.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      2184513..2185301 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_416607.1 hydroxyethylthiazole kinase [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_416607.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A8T6BZZ0
      Conserved Domains (1) summary
      pfam02110
      Location:15 → 259
      HK; Hydroxyethylthiazole kinase family
    Nucleotide Protein
    Heading Accession and Version

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