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    rluA 23S rRNA pseudouridine(746) and tRNA pseudouridine(32) synthase [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 946262, updated on 17-Dec-2024

    Summary

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    Official Symbol
    rluA
    Official Full Name
    23S rRNA pseudouridine(746) and tRNA pseudouridine(32) synthase
    Primary source
    ECOCYC:EG12609
    Locus tag
    b0058
    See related
    ASAP:ABE-0000196
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK0059; yabO
    Summary
    RluA is the pseudouridine synthase that catalyzes formation of pseudouridine at position 746 in 23S rRNA and at position 32 in tRNAPhe . [More information is available at EcoCyc: EG12609].
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    Genomic context

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    Sequence:
    NC_000913.3 (59687..60346, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene co-chaperone protein DjlA Neighboring gene putative uncharacterized protein YabP Neighboring gene protein YabQ Neighboring gene RNA polymerase-binding ATPase and RNAP recycling factor Neighboring gene DNA polymerase II

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Crystal structure of pseudouridine synthase RluA: indirect sequence readout through protein-induced RNA structure. Hoang C, et al. Mol Cell, 2006 Nov 17. PMID 17188032
    2. Mechanistic investigations of the pseudouridine synthase RluA using RNA containing 5-fluorouridine. Hamilton CS, et al. Biochemistry, 2006 Oct 3. PMID 17002302, Free PMC Article
    3. Artificial polypeptide scaffold for protein immobilization. Zhang K, et al. J Am Chem Soc, 2005 Jul 27. PMID 16028902
    4. A frequently amplified region in Leishmania contains a gene conserved in prokaryotes and eukaryotes. Myler PJ, et al. Gene, 1994 Oct 21. PMID 7958944
    5. Identification of new RNA modifying enzymes by iterative genome search using known modifying enzymes as probes. Gustafsson C, et al. Nucleic Acids Res, 1996 Oct 1. PMID 8871555, Free PMC Article

    See all (23) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. RluA displayed a rate constant of pseudouridine formation of 0.7 sec(-1).
      Title: Pre-steady-state kinetic analysis of the three Escherichia coli pseudouridine synthases TruB, TruA, and RluA reveals uniformly slow catalysis.
    2. N-terminus verified by Edman degradation on mature peptide
      Title: A dual-specificity pseudouridine synthase: an Escherichia coli synthase purified and cloned on the basis of its specificity for psi 746 in 23S RNA is also specific for psi 32 in tRNA(phe).
    3. The 2.05 A resolution structure of RluA bound to a substrate RNA comprising the anticodon stem loop of tRNA(Phe) reveals that enzyme binding induces a dramatic reorganization of the RNA.
      Title: Crystal structure of pseudouridine synthase RluA: indirect sequence readout through protein-induced RNA structure.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables 23S rRNA pseudouridine(746) synthase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables RNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables catalytic activity, acting on RNA IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables isomerase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables pseudouridine synthase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables pseudouridine synthase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables pseudouridine synthase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables pseudouridine synthase activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables rRNA pseudouridine synthase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables tRNA pseudouridine synthase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables tRNA pseudouridine(32) synthase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    acts_upstream_of_or_within RNA modification IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in RNA processing IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in enzyme-directed rRNA pseudouridine synthesis IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within enzyme-directed rRNA pseudouridine synthesis IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in pseudouridine synthesis IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within pseudouridine synthesis IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in rRNA processing IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within rRNA processing IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within rRNA pseudouridine synthesis IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in tRNA processing IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within tRNA processing IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within tRNA pseudouridine synthesis IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    23S rRNA pseudouridine(746) and tRNA pseudouridine(32) synthase
    NP_414600.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      59687..60346 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_414600.1 23S rRNA pseudouridine(746) and tRNA pseudouridine(32) synthase [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_414600.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A2W6PA86
      Conserved Domains (1) summary
      PRK10158
      Location:1 → 219
      PRK10158; bifunctional tRNA pseudouridine(32) synthase/23S rRNA pseudouridine(746) synthase RluA
    Nucleotide Protein
    Heading Accession and Version

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