Pck1 phosphoenolpyruvate carboxykinase 1 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Pck1provided by RGD
Official Full Name: phosphoenolpyruvate carboxykinase 1provided by RGD
Primary source: RGD:3267
See related: EnsemblRapid:ENSRNOG00000028616 AllianceGenome:RGD:3267
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: GTP; PCK; Pepck; PEPCK-C; RATPEPCK
Summary: Enables several functions, including anion binding activity; manganese ion binding activity; and nucleoside diphosphate kinase activity. Involved in several processes, including cellular response to carbohydrate stimulus; cellular response to peptide hormone stimulus; and cellular response to salt stress. Located in cytoplasm. Used to study type 2 diabetes mellitus. Biomarker of metabolic dysfunction-associated steatohepatitis. Human ortholog(s) of this gene implicated in Alzheimer's disease and type 2 diabetes mellitus. Orthologous to human PCK1 (phosphoenolpyruvate carboxykinase 1). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Biased expression in Kidney (RPKM 8650.8), Liver (RPKM 2902.4) and 2 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 3q42

Exon count:: 10

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	3	NC_086021.1 (182348572..182354521)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	3	NC_051338.1 (161930256..161936205)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	3	NC_005102.4 (171213936..171219885)

Chromosome 3 - NC_086021.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Ameliorative Effect of Oxytocin on FBN1 and PEPCK Gene Expression, and Behavioral Patterns in Rats' Obesity-Induced Diabetes.
Title: Ameliorative Effect of Oxytocin on FBN1 and PEPCK Gene Expression, and Behavioral Patterns in Rats' Obesity-Induced Diabetes.
Cytosolic phosphoenolpyruvate carboxykinase is expressed in alpha-cells from human and murine pancreas.
Title: Cytosolic phosphoenolpyruvate carboxykinase is expressed in α-cells from human and murine pancreas.
The relationship between PEPCK metabolism in the urinary tract and insulin resistance and diabetes is reported.
Title: Phosphoenolpyruvate carboxykinase in urine exosomes reflect impairment in renal gluconeogenesis in early insulin resistance and diabetes.
PEPCK1 is integral to coordinating cellular metabolism in the liver and adipose tissue, although it does not directly effect hepatic glucose production or adipose glyceroneogenesis.
Title: PEPCK1 Antisense Oligonucleotide Prevents Adiposity and Impairs Hepatic Glycogen Synthesis in High-Fat Male Fed Rats.
Rap1 activation inhibits CNV through regulating barrier integrity and ROS generation of RPE in vivo, and selectively activating Rap1 may be a way to reduce vision loss from CNV
Title: Activation of the Small GTPase Rap1 Inhibits Choroidal Neovascularization by Regulating Cell Junctions and ROS Generation in Rats.
Peroxynitrite-induced coronary vascular dysfunction may be mediated, at least in part, through increased expressions and activities of RhoA, ROCK1, and ROCK2.
Title: RhoA/rock signaling mediates peroxynitrite-induced functional impairment of Rat coronary vessels.
GBS can reduce blood glucose in T2DM rats while improving glucose tolerance and hyperglycemia, and the mechanism appears to be associated with a decrease of hepatic PEPCK mRNA and protein expression.
Title: Hepatic phosphoenolpyruvate carboxykinase expression after gastric bypass surgery in rats with type 2 diabetes mellitus.
PEPCK is downregulated and has a role in reduction of blood glucose and gluconeogenesis after phenobarbital administration in rats
Title: Phenobarbital reduces blood glucose and gluconeogenesis through down-regulation of phosphoenolpyruvate carboxykinase (GTP) gene expression in rats.
It observed lower expression of hepatic PEPCK mRNA, in the magnesium-deficient rats, thus suggesting a possible compensatory mechanism to diminish glycogenesis. A low-magnesium diet alters glucocorticoid metabolism, which leads to endothelial damage.
Title: Down-regulation of hepatic phosphoenolpyruvate carboxykinase expression in magnesium-deficient rats.
Leptin activates NOS III and induces nitric oxide that nitrates PEPCK-C to reduce its level and glyceroneogenesis.
Title: Rapid nitration of adipocyte phosphoenolpyruvate carboxykinase by leptin reduces glyceroneogenesis and induces fatty acid release.

Submit: New GeneRIF Correction See all GeneRIFs (43)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

PMC133887P2 (e-PCR)
- UniSTS:270676

PMC152262P3 (e-PCR)
- UniSTS:271211

Pck1 (e-PCR), detects polymorphism
- UniSTS:546923

d9mit10 (e-PCR)
- UniSTS:122481

PMC86435P1 (e-PCR)
- UniSTS:273523

d3arb24 (e-PCR)
- UniSTS:121771

d3mgh26 (e-PCR)
- UniSTS:121785

Pck1 (e-PCR), detects polymorphism
- UniSTS:526051

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables GDP binding	IDA Inferred from Direct Assay more info	PubMed
enables GTP binding	IDA Inferred from Direct Assay more info	PubMed
enables GTP binding	ISO Inferred from Sequence Orthology more info
enables carboxylic acid binding	IDA Inferred from Direct Assay more info	PubMed
enables carboxylic acid binding	ISO Inferred from Sequence Orthology more info
enables magnesium ion binding	ISO Inferred from Sequence Orthology more info
enables magnesium ion binding	ISS Inferred from Sequence or Structural Similarity more info	PubMed
enables manganese ion binding	IBA Inferred from Biological aspect of Ancestor more info
enables manganese ion binding	IDA Inferred from Direct Assay more info	PubMed
enables manganese ion binding	ISO Inferred from Sequence Orthology more info
enables nucleoside diphosphate kinase activity	EXP Inferred from Experiment more info	PubMed
enables phosphoenolpyruvate carboxykinase (GTP) activity	IBA Inferred from Biological aspect of Ancestor more info
enables phosphoenolpyruvate carboxykinase (GTP) activity	IDA Inferred from Direct Assay more info	PubMed
enables phosphoenolpyruvate carboxykinase (GTP) activity	ISO Inferred from Sequence Orthology more info
enables phosphoenolpyruvate carboxykinase activity	IDA Inferred from Direct Assay more info	PubMed
enables phosphoenolpyruvate carboxykinase activity	ISO Inferred from Sequence Orthology more info
enables protein serine kinase activity (using GTP as donor)	ISO Inferred from Sequence Orthology more info
enables protein serine kinase activity (using GTP as donor)	ISS Inferred from Sequence or Structural Similarity more info

Process	Evidence Code	Pubs
involved_in cellular hyperosmotic response	IEP Inferred from Expression Pattern more info	PubMed
involved_in cellular hyperosmotic salinity response	IEP Inferred from Expression Pattern more info	PubMed
involved_in cellular hypotonic response	IEP Inferred from Expression Pattern more info	PubMed
involved_in cellular hypotonic salinity response	IEP Inferred from Expression Pattern more info	PubMed
involved_in cellular response to cAMP	IEP Inferred from Expression Pattern more info	PubMed
involved_in cellular response to dexamethasone stimulus	IBA Inferred from Biological aspect of Ancestor more info
involved_in cellular response to fructose stimulus	IEP Inferred from Expression Pattern more info	PubMed
involved_in cellular response to glucagon stimulus	IEP Inferred from Expression Pattern more info	PubMed
involved_in cellular response to glucose stimulus	IBA Inferred from Biological aspect of Ancestor more info
involved_in cellular response to glucose stimulus	IDA Inferred from Direct Assay more info	PubMed
involved_in cellular response to glucose stimulus	ISO Inferred from Sequence Orthology more info
involved_in cellular response to hypoxia	IEP Inferred from Expression Pattern more info	PubMed
involved_in cellular response to insulin stimulus	IBA Inferred from Biological aspect of Ancestor more info
involved_in cellular response to insulin stimulus	IEP Inferred from Expression Pattern more info	PubMed
involved_in cellular response to insulin stimulus	ISO Inferred from Sequence Orthology more info
involved_in cellular response to insulin stimulus	ISS Inferred from Sequence or Structural Similarity more info
involved_in cellular response to interleukin-1	IEP Inferred from Expression Pattern more info	PubMed
involved_in cellular response to phorbol 13-acetate 12-myristate	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within cellular response to potassium ion starvation	ISO Inferred from Sequence Orthology more info
involved_in cellular response to raffinose	IEP Inferred from Expression Pattern more info	PubMed
involved_in cellular response to retinoic acid	IEP Inferred from Expression Pattern more info	PubMed
involved_in cellular response to tumor necrosis factor	IEP Inferred from Expression Pattern more info	PubMed
involved_in gluconeogenesis	IBA Inferred from Biological aspect of Ancestor more info
involved_in gluconeogenesis	IDA Inferred from Direct Assay more info	PubMed
involved_in gluconeogenesis	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within gluconeogenesis	ISO Inferred from Sequence Orthology more info
involved_in gluconeogenesis	ISO Inferred from Sequence Orthology more info
involved_in gluconeogenesis	TAS Traceable Author Statement more info	PubMed
involved_in glucose homeostasis	IDA Inferred from Direct Assay more info	PubMed
involved_in glucose metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in glucose metabolic process	ISO Inferred from Sequence Orthology more info
involved_in glyceraldehyde-3-phosphate biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in glycerol biosynthetic process from pyruvate	IBA Inferred from Biological aspect of Ancestor more info
involved_in glycerol biosynthetic process from pyruvate	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within glycerol biosynthetic process from pyruvate	ISO Inferred from Sequence Orthology more info
involved_in hepatocyte differentiation	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within lipid metabolic process	ISO Inferred from Sequence Orthology more info
involved_in oxaloacetate metabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in oxaloacetate metabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in oxaloacetate metabolic process	ISO Inferred from Sequence Orthology more info
involved_in peptidyl-serine phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in peptidyl-serine phosphorylation	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of lipid biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of memory T cell differentiation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of memory T cell differentiation	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within positive regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
involved_in propionate catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of lipid biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in regulation of lipid biosynthetic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in response to activity	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within response to bacterium	ISO Inferred from Sequence Orthology more info
involved_in response to insulin	ISO Inferred from Sequence Orthology more info
involved_in response to insulin	ISS Inferred from Sequence or Structural Similarity more info	PubMed
involved_in response to interleukin-6	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to lipid	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to lipopolysaccharide	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to methionine	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to nutrient levels	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to starvation	IBA Inferred from Biological aspect of Ancestor more info
involved_in tricarboxylic acid metabolic process	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
is_active_in cytosol	ISO Inferred from Sequence Orthology more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in cytosol	ISS Inferred from Sequence or Structural Similarity more info
located_in cytosol	TAS Traceable Author Statement more info
located_in endoplasmic reticulum	ISO Inferred from Sequence Orthology more info
located_in endoplasmic reticulum	ISS Inferred from Sequence or Structural Similarity more info
is_active_in mitochondrion	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: phosphoenolpyruvate carboxykinase, cytosolic [GTP]

Names: phosphoenolpyruvate carboxykinase (GTP); phosphoenolpyruvate carboxykinase 1 (soluble); phosphoenolpyruvate carboxykinase 1, cytosolic; phosphoenolpyruvate carboxykinase, cytosolic (GTP); phosphoenolpyruvate carboxylase; serine-protein kinase PCK1

NP_942075.1

EC 4.1.1.32

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_198780.3 → NP_942075.1 phosphoenolpyruvate carboxykinase, cytosolic [GTP]

See identical proteins and their annotated locations for NP_942075.1

Status: VALIDATED

Source sequence(s)

BC081900, JAXUCZ010000003

UniProtKB/Swiss-Prot

P07379

UniProtKB/TrEMBL

A6KKZ5

Related

ENSRNOP00000030913.5, ENSRNOT00000031586.8

Conserved Domains (1) summary

cd00819
Location:27 → 615

PEPCK_GTP; Phosphoenolpyruvate carboxykinase (PEPCK), a critical gluconeogenic enzyme, catalyzes the first committed step in the diversion of tricarboxylic acid cycle intermediates toward gluconeogenesis. It catalyzes the reversible decarboxylation and ...