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    PRP22 DEAH-box ATP-dependent RNA helicase PRP22 [ Saccharomyces cerevisiae S288C ]

    Gene ID: 856732, updated on 9-Dec-2024

    Summary

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    Official Symbol
    PRP22
    Official Full Name
    DEAH-box ATP-dependent RNA helicase PRP22
    Primary source
    SGD:S000000815
    Locus tag
    YER013W
    See related
    AllianceGenome:SGD:S000000815; FungiDB:YER013W; VEuPathDB:YER013W
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Summary
    Enables RNA helicase activity. Contributes to second spliceosomal transesterification activity. Involved in generation of catalytic spliceosome for second transesterification step and spliceosomal complex disassembly. Part of U2-type catalytic step 2 spliceosome and U2-type post-spliceosomal complex. Orthologous to human DHX8 (DEAH-box helicase 8). [provided by Alliance of Genome Resources, Dec 2024]
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    Genomic context

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    See PRP22 in Genome Data Viewer
    Location:
    chromosome: V
    Exon count:
    1
    Sequence:
    Chromosome: V; NC_001137.3 (178841..182278)

    Chromosome V - NC_001137.3Genomic Context describing neighboring genes Neighboring gene tRNA-Glu Neighboring gene proteasome core particle subunit beta 4 Neighboring gene oxygen-dependent protoporphyrinogen oxidase Neighboring gene Bud25p Neighboring gene medium-chain fatty acid-CoA ligase FAA2

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_001137.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Prp22, a DExH-box RNA helicase, plays two distinct roles in yeast pre-mRNA splicing. Schwer B, et al. EMBO J, 1998 Apr 1. PMID 9524130, Free PMC Article
    2. The DEAH-box protein PRP22 is an ATPase that mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes. Wagner JD, et al. EMBO J, 1998 May 15. PMID 9582286, Free PMC Article
    3. Functional domains of the yeast splicing factor Prp22p. Schneider S, et al. J Biol Chem, 2001 Jun 15. PMID 11283007
    4. Detecting circRNA in purified spliceosomal P complex. Shi S, et al. Methods, 2021 Dec. PMID 33577981, Free PMC Article
    5. Both catalytic steps of nuclear pre-mRNA splicing are reversible. Tseng CK, et al. Science, 2008 Jun 27. PMID 18583613

    See all (82) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. At the catalytic stage of splicing, suboptimal splice sites are repressed by the DEAH-box ATPases Prp16 and Prp22. study shows that these ATPases function further by enabling the spliceosome to search for and utilize alternative branch sites and 3' splice sites.
      Title: Spliceosomal DEAH-Box ATPases Remodel Pre-mRNA to Activate Alternative Splice Sites.
    2. C-terminal domain of the spliceosomal helicase Prp22
      Title: Structural analysis of the C-terminal domain of the spliceosomal helicase Prp22.
    3. NTR complex catalyzes the disassembly of affinity-purified spliceosomes arrested specifically after the ATP-dependent action of DEAH-box Prp2, Prp16, or Prp22 but not at steps before the action of these ATPases or upon their binding to the spliceosome.
      Title: Link of NTR-mediated spliceosome disassembly with DEAH-box ATPases Prp2, Prp16, and Prp22.
    4. Prp22 unwinds duplexes with 3' overhangs; the 3' -> 5' directionality has implications for positioning of Prp22 on the RNA target
      Title: Characterization of the NTPase, RNA-binding, and RNA helicase activities of the DEAH-box splicing factor Prp22.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ATP hydrolysis activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ATP-dependent H2AZ histone chaperone activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ATP-dependent H3-H4 histone complex chaperone activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ATP-dependent activity, acting on RNA IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA clamp loader activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables RNA binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables RNA helicase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables RNA helicase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables RNA helicase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables chromatin extrusion motor activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables cohesin loader activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables helicase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables hydrolase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables nucleic acid binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    contributes_to second spliceosomal transesterification activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    contributes_to second spliceosomal transesterification activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in RNA processing IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in RNA splicing IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in chromatin looping IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in chromatin remodeling IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in generation of catalytic spliceosome for second transesterification step IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in generation of catalytic spliceosome for second transesterification step IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in mRNA processing IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in mRNA splicing, via spliceosome IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in mRNA splicing, via spliceosome IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in protein-containing complex assembly IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in ribonucleoprotein complex biogenesis IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in spliceosomal complex disassembly IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    part_of U2-type catalytic step 2 spliceosome IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    part_of U2-type post-spliceosomal complex IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    part_of catalytic step 2 spliceosome IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in nucleus IEA
    Inferred from Electronic Annotation
    more info
    		  
    part_of ribonucleoprotein complex IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    DEAH-box ATP-dependent RNA helicase PRP22
    NP_010929.3
    • DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase; associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes; required for proofreading the exon ligation reaction

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001137.3 Reference assembly

      Range
      178841..182278
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001178904.3 → NP_010929.3  TPA: DEAH-box ATP-dependent RNA helicase PRP22 [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_010929.3

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      D3DLR0, P24384
      UniProtKB/TrEMBL
      B3LS25, B5VHE4, C7GKJ1, N1P5R0
      Conserved Domains (2) summary
      COG1185
      Location:1 → 423
      Pnp; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase) [Translation, ribosomal structure and biogenesis]
      COG1643
      Location:456 → 1145
      HrpA; HrpA-like RNA helicase [Translation, ribosomal structure and biogenesis]
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P24384.1

    Gene LinkOut

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