PBS2 mitogen-activated protein kinase kinase PBS2 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: PBS2
Official Full Name: mitogen-activated protein kinase kinase PBS2
Primary source: SGD:S000003664
Locus tag: YJL128C
See related: AllianceGenome:SGD:S000003664; FungiDB:YJL128C; VEuPathDB:YJL128C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: HOG4; SFS4; SSK4
Summary: Enables MAP kinase kinase activity and MAP-kinase scaffold activity. Involved in several processes, including cellular response to osmotic stress; p38MAPK cascade; and protein import into nucleus. Located in cellular bud neck; cellular bud tip; and cytoplasm. Human ortholog(s) of this gene implicated in several diseases, including Pick's disease; colorectal cancer (multiple); female reproductive organ cancer (multiple); progressive supranuclear palsy; and respiratory system cancer (multiple). Orthologous to several human genes including MAP2K3 (mitogen-activated protein kinase kinase 3) and MAP2K7 (mitogen-activated protein kinase kinase 7). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See PBS2 in Genome Data Viewer

Location:: chromosome: X

Exon count:: 1

Sequence:: Chromosome: X; NC_001142.9 (178097..180103, complement)

Chromosome X - NC_001142.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001142.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Data show that that two alternative MAPKKs, Pbs2 and Mkk2, can be forced to functionally replace the mating MAPKK Ste7, but only if the proper set of recruitment interactions are grafted onto their catalytic domains.
Title: Recruitment interactions can override catalytic interactions in determining the functional identity of a protein kinase.
hypersensitivity to salt and other stress conditions was due to the deletion of 5 base pairs close to position 936 bp in the PBS2 gene essential for HOG signal pathway regulation under salt stress
Title: [Identification of the gene correlated with salt stress in the Saccharomyces cerevisiae 263-H9 mutant].
In the N-terminal noncatalytic region of Pbs2, there is a specific Hog1-binding domain, HBD-1.
Title: Two adjacent docking sites in the yeast Hog1 mitogen-activated protein (MAP) kinase differentially interact with the Pbs2 MAP kinase kinase and the Ptp2 protein tyrosine phosphatase.
Here we show that by replacing C-terminal region of Dpbs2p with the homologous region of Pbs2p we could abrogate partial complementation exhibited by Dpbs2p and this was achieved due to increase in nuclear translocation of Hog1p.
Title: Evidence that C-terminal non-kinase domain of Pbs2p has a role in high osmolarity-induced nuclear localization of Hog1p.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables JUN kinase kinase activity	IEA Inferred from Electronic Annotation more info
enables MAP kinase kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables MAP kinase kinase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables MAP kinase kinase activity	ISS Inferred from Sequence or Structural Similarity more info	PubMed
enables MAP-kinase scaffold activity	IPI Inferred from Physical Interaction more info	PubMed
enables histone H2AXY142 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3Y41 kinase activity	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase activity	HDA more info	PubMed
enables protein kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein tyrosine kinase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in JNK cascade	IEA Inferred from Electronic Annotation more info
involved_in MAPK cascade	IBA Inferred from Biological aspect of Ancestor more info
involved_in MAPK cascade	IEA Inferred from Electronic Annotation more info
involved_in actin filament organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cellular hyperosmotic response	IBA Inferred from Biological aspect of Ancestor more info
involved_in cellular hyperosmotic response	IGI Inferred from Genetic Interaction more info	PubMed
involved_in chromatin remodeling	IEA Inferred from Electronic Annotation more info
involved_in hyperosmotic response	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in osmosensory signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in osmosensory signaling pathway via Sho1 osmosensor	IPI Inferred from Physical Interaction more info	PubMed
involved_in p38MAPK cascade	IGI Inferred from Genetic Interaction more info	PubMed
involved_in p38MAPK cascade	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein import into nucleus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to antibiotic	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in cellular bud neck	IDA Inferred from Direct Assay more info	PubMed
located_in cellular bud tip	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoplasmic stress granule	HDA more info	PubMed

General protein information

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Preferred Names: mitogen-activated protein kinase kinase PBS2

NP_012407.2

EC 2.7.12.2

MAP kinase kinase of the HOG signaling pathway; activated under severe osmotic stress; mitophagy-specific regulator; plays a role in regulating Ty1 transposition

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001142.9 Reference assembly

Range

178097..180103 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001181561.2 → NP_012407.2 TPA: mitogen-activated protein kinase kinase PBS2 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_012407.2

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VW57, P08018

UniProtKB/TrEMBL

B3LPZ0, C7GQ00, C8ZB89, N1P936

Conserved Domains (1) summary

cd06622
Location:358 → 644

PKc_PBS2_like; Catalytic domain of fungal PBS2-like dual-specificity Mitogen-Activated Protein Kinase Kinases