Pfkl phosphofructokinase, liver, B-type [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Pfklprovided by MGI
Official Full Name: phosphofructokinase, liver, B-typeprovided by MGI
Primary source: MGI:MGI:97547
See related: Ensembl:ENSMUSG00000020277 AllianceGenome:MGI:97547
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: PFK-B; PFK-L; ATP-PFK
Summary: Enables 6-phosphofructokinase activity. Involved in glycolytic process through fructose-6-phosphate. Acts upstream of or within fructose 6-phosphate metabolic process; negative regulation of insulin secretion; and response to glucose. Located in cytosol. Is expressed in several structures, including alimentary system; brain; genitourinary system; musculoskeletal system; and sensory organ. Orthologous to human PFKL (phosphofructokinase, liver type). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Ubiquitous expression in colon adult (RPKM 59.9), ovary adult (RPKM 45.7) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 10 C1; 10 39.72 cM

Exon count:: 22

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	10	NC_000076.7 (77822781..77845641, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	10	NC_000076.6 (77986947..78009807, complement)

Chromosome 10 - NC_000076.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Caveolin-1 depletion attenuates hepatic fibrosis via promoting SQSTM1-mediated PFKL degradation in HSCs.
Title: Caveolin-1 depletion attenuates hepatic fibrosis via promoting SQSTM1-mediated PFKL degradation in HSCs.
The KLF7/PFKL/ACADL axis modulates cardiac metabolic remodelling during cardiac hypertrophy in male mice.
Title: The KLF7/PFKL/ACADL axis modulates cardiac metabolic remodelling during cardiac hypertrophy in male mice.
TAp73 deficiency results in a pronounced reduction in tumorigenic potential, which can be rescued by forced PFKL expression.
Title: TAp73-induced phosphofructokinase-1 transcription promotes the Warburg effect and enhances cell proliferation.
insulin, the most known regulator of glucose consumption, specifically regulates the expression of PFKL and PFKM, which impact the regulation of glycolysis in the cell.
Title: Insulin specifically regulates expression of liver and muscle phosphofructokinase isoforms.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (1)
Targeted (1)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Pfkl (e-PCR), detects polymorphism
- UniSTS:259128

RH124641 (e-PCR)
- UniSTS:161463

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables 6-phosphofructokinase activity	IDA Inferred from Direct Assay more info	PubMed
contributes_to 6-phosphofructokinase activity	ISO Inferred from Sequence Orthology more info
enables 6-phosphofructokinase activity	ISO Inferred from Sequence Orthology more info
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP binding	ISO Inferred from Sequence Orthology more info
enables fructose binding	IEA Inferred from Electronic Annotation more info
enables fructose binding	ISO Inferred from Sequence Orthology more info
enables fructose-6-phosphate binding	IBA Inferred from Biological aspect of Ancestor more info
enables fructose-6-phosphate binding	IEA Inferred from Electronic Annotation more info
enables fructose-6-phosphate binding	ISO Inferred from Sequence Orthology more info
enables identical protein binding	IEA Inferred from Electronic Annotation more info
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables kinase binding	IEA Inferred from Electronic Annotation more info
enables kinase binding	ISO Inferred from Sequence Orthology more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables monosaccharide binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in canonical glycolysis	IBA Inferred from Biological aspect of Ancestor more info
involved_in fructose 1,6-bisphosphate metabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in fructose 1,6-bisphosphate metabolic process	ISO Inferred from Sequence Orthology more info
involved_in fructose 1,6-bisphosphate metabolic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in fructose 6-phosphate metabolic process	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within fructose 6-phosphate metabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in fructose 6-phosphate metabolic process	ISO Inferred from Sequence Orthology more info
involved_in glycolytic process	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within glycolytic process through fructose-6-phosphate	IDA Inferred from Direct Assay more info	PubMed
involved_in glycolytic process through fructose-6-phosphate	IDA Inferred from Direct Assay more info	PubMed
involved_in glycolytic process through fructose-6-phosphate	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within negative regulation of insulin secretion	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within response to glucose	IDA Inferred from Direct Assay more info	PubMed
involved_in response to glucose	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
part_of 6-phosphofructokinase complex	IBA Inferred from Biological aspect of Ancestor more info
part_of 6-phosphofructokinase complex	IEA Inferred from Electronic Annotation more info
part_of 6-phosphofructokinase complex	ISO Inferred from Sequence Orthology more info
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
is_active_in membrane	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: ATP-dependent 6-phosphofructokinase, liver type

Names: 6-phosphofructokinase type B; 6-phosphofructokinase, liver type; phosphofructo-1-kinase isozyme B; phosphofructokinase 1; phosphohexokinase

NP_001345722.1

EC 2.7.1.11

NP_032852.2

EC 2.7.1.11

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001358793.1 → NP_001345722.1 ATP-dependent 6-phosphofructokinase, liver type isoform 2

Status: VALIDATED

Source sequence(s)

AC153507, AK030892, AK081313, BP766623, BY070321, EL605376

UniProtKB/TrEMBL

Q8CD98

Conserved Domains (1) summary

cl00204
Location:1 → 458

PFK; Phosphofructokinase, a key regulatory enzyme in glycolysis, catalyzes the phosphorylation of fructose-6-phosphate to fructose-1,6-biphosphate. The members belong to PFK family that includes ATP- and pyrophosphate (PPi)- dependent phosphofructokinases. ...
NM_008826.5 → NP_032852.2 ATP-dependent 6-phosphofructokinase, liver type isoform 1

Status: VALIDATED

Source sequence(s)

AK030892, AK081313, BP766623, BY070321

Consensus CDS

CCDS35955.1

UniProtKB/Swiss-Prot

P12382, Q8VDX7

Related

ENSMUSP00000020522.9, ENSMUST00000020522.9

Conserved Domains (2) summary

cd00764
Location:13 → 773

Eukaryotic_PFK; Phosphofructokinase, a key regulatory enzyme in glycolysis, catalyzes the phosphorylation of fructose-6-phosphate to fructose-1,6-biphosphate. The members belong to a subfamily of the PFKA family (cd00363) and include eukaryotic ATP-dependent ...

TIGR02478
Location:16 → 755

6PF1K_euk; 6-phosphofructokinase, eukaryotic type