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    glmS L-glutamine--D-fructose-6-phosphate aminotransferase [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 948241, updated on 3-Dec-2024

    Summary

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    Official Symbol
    glmS
    Official Full Name
    L-glutamine--D-fructose-6-phosphate aminotransferase
    Primary source
    ECOCYC:EG10382
    Locus tag
    b3729
    See related
    ASAP:ABE-0012198
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK3722
    Summary
    GlmS synthesis s negatively feedback regulated by glucosamine-6-phosphate, which probably causes YhbJ to cleave and inactivate the GlmZ sRNA; GlmZ upregulates glmS mRNA post-translationally (Kalamorz, 2007). [More information is available at EcoGene: EG10382]. L-glutamine:D-fructose-6-phosphate aminotransferase, or GFAT, catalyzes the first step in hexosamine biosynthesis. [More information is available at EcoCyc: EG10382].
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    Genomic context

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    Sequence:
    NC_000913.3 (3911839..3913668, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene phosphate ABC transporter membrane subunit PstA Neighboring gene phosphate ABC transporter membrane subunit PstC Neighboring gene phosphate ABC transporter periplasmic binding protein Neighboring gene fused N-acetylglucosamine-1-phosphate uridyltransferase and glucosamine-1-phosphate acetyltransferase Neighboring gene ATP synthase F1 complex subunit epsilon

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Glutamate gamma-semialdehyde as a natural transition state analogue inhibitor of Escherichia coli glucosamine-6-phosphate synthase. Bearne SL, et al. Biochemistry, 1995 Sep 12. PMID 7547881
    2. Metabolic engineering of Escherichia coli for industrial production of glucosamine and N-acetylglucosamine. Deng MD, et al. Metab Eng, 2005 May. PMID 15885618
    3. Glucosamine metabolism. V. Enzymatic synthesis of glucosamine 6-phosphate. GHOSH S, et al. J Biol Chem, 1960 May. PMID 13827775
    4. Isomerase activity of the C-terminal fructose-6-phosphate binding domain of glucosamine-6-phosphate synthase from Escherichia coli. Todorova R. J Enzyme Inhib, 2001 Oct. PMID 11916143
    5. Channeling of ammonia in glucosamine-6-phosphate synthase. Teplyakov A, et al. J Mol Biol, 2001 Nov 9. PMID 11700065

    See all (55) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. accumulation of GlmZ* prevents complete GlmZ turnover. This mechanism may serve to adjust a robust glmS basal expression level that is buffered against fluctuations in RapZ availability.
      Title: Feedback regulation of small RNA processing by the cleavage product.
    2. An effective technique for the construction of GM (genetically modified) bacteria without an antibiotic resistant marker, and the construction of GM yeasts to be applied to complex media.
      Title: Application of GFAT as a novel selection marker to mediate gene expression.
    3. Analysis of how GlmZ, GlmY and PAP I act together in the regulation of glmS expression.
      Title: The small RNA GlmY acts upstream of the sRNA GlmZ in the activation of glmS expression and is subject to regulation by polyadenylation in Escherichia coli.
    4. The biological function of GlmZ is to positively control the glmS mRNA in response to GlcN-6-P concentrations and that YhbJ negatively regulates GlmZ. As in yhbJ mutants GlcN-6-P has no effect, YhbJ is essential for sensing this metabolite.
      Title: Feedback control of glucosamine-6-phosphate synthase GlmS expression depends on the small RNA GlmZ and involves the novel protein YhbJ in Escherichia coli.
    5. N-terminus verified by Edman degradation on mature peptide
      Title: Glucosamine synthetase from Escherichia coli: purification, properties, and glutamine-utilizing site location.
    6. Ammonia channeling in bacterial glucosamine-6-phosphate synthase (Glms).
      Title: Ammonia channeling in bacterial glucosamine-6-phosphate synthase (Glms): molecular dynamics simulations and kinetic studies of protein mutants.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables carbohydrate derivative binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables glutamine-fructose-6-phosphate transaminase (isomerizing) activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables glutamine-fructose-6-phosphate transaminase (isomerizing) activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables glutamine-fructose-6-phosphate transaminase (isomerizing) activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables transaminase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    acts_upstream_of_or_within UDP-N-acetylglucosamine biosynthetic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in UDP-N-acetylglucosamine metabolic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in carbohydrate derivative biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in carbohydrate metabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in fructose 6-phosphate metabolic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in protein N-linked glycosylation IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    Component Evidence Code Pubs
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cytosol HDA PubMed 
    is_active_in cytosol IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    L-glutamine--D-fructose-6-phosphate aminotransferase
    NP_418185.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      3911839..3913668 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_418185.1 L-glutamine--D-fructose-6-phosphate aminotransferase [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_418185.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      Q0P6L2
      Conserved Domains (1) summary
      PRK00331
      Location:1 → 609
      PRK00331; isomerizing glutamine--fructose-6-phosphate transaminase
    Nucleotide Protein
    Heading Accession and Version

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