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    thiS sulfur carrier protein ThiS [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 2847702, updated on 3-Dec-2024

    Summary

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    Official Symbol
    thiS
    Official Full Name
    sulfur carrier protein ThiS
    Primary source
    ECOCYC:G1
    Locus tag
    b4407
    See related
    ASAP:ABE-0023907
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK3983; thiG1
    Summary
    The ThiS N-terminus has been verified by MS/MS sequencing, the Met is not clipped and the measured mass is 7310.74 (Kelleher, 1998). [More information is available at EcoGene: EG14363]. ThiS is the sulfur source for the thiazole moiety in thiamin biosynthesis. [More information is available at EcoCyc: G1].
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    Genomic context

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    Sequence:
    NC_000913.3 (4192637..4192837, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene ncRNA Neighboring gene 2-iminoacetate synthase Neighboring gene 1-deoxy-D-xylulose 5-phosphate:thiol sulfurtransferase Neighboring gene sulfur carrier protein ThiS adenylyltransferase Neighboring gene thiamine phosphate synthase Neighboring gene phosphomethylpyrimidine synthase

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Overexpression of recombinant proteins with a C-terminal thiocarboxylate: implications for protein semisynthesis and thiamin biosynthesis. Kinsland C, et al. Protein Sci, 1998 Aug. PMID 10082383, Free PMC Article
    2. Heat shock transcription factor δ³² is targeted for degradation via an ubiquitin-like protein ThiS in Escherichia coli. Xu X, et al. Biochem Biophys Res Commun, 2015 Apr 3. PMID 25721662
    3. Structure of the Escherichia coli ThiS-ThiF complex, a key component of the sulfur transfer system in thiamin biosynthesis. Lehmann C, et al. Biochemistry, 2006 Jan 10. PMID 16388576, Free PMC Article
    4. The enzymology of sulfur activation during thiamin and biotin biosynthesis. Begley TP, et al. Curr Opin Chem Biol, 1999 Oct. PMID 10508664
    5. Thiamin biosynthesis in Escherichia coli. Identification of ThiS thiocarboxylate as the immediate sulfur donor in the thiazole formation. Taylor SV, et al. J Biol Chem, 1998 Jun 26. PMID 9632726

    See all (15) citations in PubMed

    GeneRIFs: Gene References Into Functions

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables nucleotide binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables sulfur carrier activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in thiamine biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in thiamine diphosphate biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within thiazole biosynthetic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Component Evidence Code Pubs
    part_of adenylyltransferase complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    part_of sulfurtransferase complex NAS
    Non-traceable Author Statement
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    sulfur carrier protein ThiS

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      4192637..4192837 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. YP_026279.1 sulfur carrier protein ThiS [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for YP_026279.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A8T5ZBV6
      Conserved Domains (1) summary
      PRK08053
      Location:1 → 66
      PRK08053; sulfur carrier protein ThiS; Provisional
    Nucleotide Protein
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