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    waaJ UDP-glucose:(glucosyl)LPS alpha-1,2-glucosyltransferase [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 948142, updated on 3-Dec-2024

    Summary

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    Official Symbol
    waaJ
    Official Full Name
    UDP-glucose:(glucosyl)LPS alpha-1,2-glucosyltransferase
    Primary source
    ECOCYC:EG11353
    Locus tag
    b3626
    See related
    ASAP:ABE-0011858
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK3616; rfaJ; waaR
    Summary
    The lipopolysaccharide of Escherichia coli K-12 consists of two major components: the hydrophobic lipid A moiety inserted into the outer membrane and the phosphorylated core oligosaccharide . [More information is available at EcoCyc: EG11353].
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    Genomic context

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    Sequence:
    NC_000913.3 (3800983..3801999, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene lipopolysaccharide core biosynthesis protein WaaZ Neighboring gene lipopolysaccharide core heptose (II) kinase Neighboring gene UDP-D-glucose:(glucosyl)LPS alpha-1,3-glucosyltransferase Neighboring gene UDP-D-galactose:(glucosyl)lipopolysaccharide-1,6-D-galactosyltransferase

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Recombinant Escherichia coli K5 strain with the deletion of waaR gene decreases the molecular weight of the heparosan capsular polysaccharide. Huang H, et al. Appl Microbiol Biotechnol, 2016 Sep. PMID 27079575
    2. The C-terminal Domain of the Escherichia coli WaaJ glycosyltransferase is important for catalytic activity and membrane association. Leipold MD, et al. J Biol Chem, 2007 Jan 12. PMID 17090541
    3. Mutations in the waaR gene of Escherichia coli which disrupt lipopolysaccharide outer core biosynthesis affect cell surface retention of group 2 capsular polysaccharides. Taylor CM, et al. J Bacteriol, 2006 Feb. PMID 16428421, Free PMC Article
    4. Four critical aspartic acid residues potentially involved in the catalytic mechanism of Escherichia coli K-12 WaaR. Shibayama K, et al. FEMS Microbiol Lett, 1999 May 1. PMID 10234827
    5. Genetic analysis of lipopolysaccharide core biosynthesis by Escherichia coli K-12: insertion mutagenesis of the rfa locus. Austin EA, et al. J Bacteriol, 1990 Sep. PMID 2168379, Free PMC Article

    See all (29) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. The waaR deletion mutant showed improvement of capsule polysaccharide yield.
      Title: Recombinant Escherichia coli K5 strain with the deletion of waaR gene decreases the molecular weight of the heparosan capsular polysaccharide.
    2. Synthesis of different glycoforms is regulated by differential expression of WaaZ (KdoIII transferase) and WaaR (glycosyltransferase).
      Title: Molecular basis of lipopolysaccharide heterogeneity in Escherichia coli: envelope stress-responsive regulators control the incorporation of glycoforms with a third 3-deoxy-α-D-manno-oct-2-ulosonic acid and rhamnose.
    3. the C-terminal domain of WaaJ plays a critical role in enzyme function
      Title: The C-terminal Domain of the Escherichia coli WaaJ glycosyltransferase is important for catalytic activity and membrane association.
    Submit: New GeneRIF Correction

    Pathways from PubChem

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    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables glycosyltransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables lipopolysaccharide 3-alpha-galactosyltransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables lipopolysaccharide glucosyltransferase I activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables lipopolysaccharide glucosyltransferase I activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables lipopolysaccharide glucosyltransferase I activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in lipopolysaccharide biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in lipopolysaccharide core region biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within lipopolysaccharide core region biosynthetic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in plasma membrane HDA PubMed 
    located_in plasma membrane IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    UDP-glucose:(glucosyl)LPS alpha-1,2-glucosyltransferase
    NP_418083.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      3800983..3801999 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_418083.1 UDP-glucose:(glucosyl)LPS alpha-1,2-glucosyltransferase [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_418083.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A3R0MXW2
      Conserved Domains (1) summary
      COG1442
      Location:26 → 338
      RfaJ; Lipopolysaccharide biosynthesis protein, LPS:glycosyltransferase [Cell wall/membrane/envelope biogenesis]
    Nucleotide Protein
    Heading Accession and Version

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