Rad51d RAD51 paralog D [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Rad51dprovided by MGI
Official Full Name: RAD51 paralog Dprovided by MGI
Primary source: MGI:MGI:1261809
See related: Ensembl:ENSMUSG00000018841 AllianceGenome:MGI:1261809
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: TRAD; R51H3; Rad51l3; Trad-d5
Summary: This gene belongs to the Rad51 gene family whose products play a major role in homologous recombination and DNA repair. The encoded protein interacts with other proteins of this family, including Rad51b, Rad51c and Xrcc2, and plays an essential role in both DNA repair and telomere maintenance. In humans, germline mutations in this gene may be associated with predisposition to ovarian cancer. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, May 2013]
Expression: Ubiquitous expression in subcutaneous fat pad adult (RPKM 3.8), bladder adult (RPKM 3.7) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 11 C; 11 50.3 cM

Exon count:: 11

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	11	NC_000077.7 (82762786..82781571, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	11	NC_000077.6 (82871960..82890624, complement)

Chromosome 11 - NC_000077.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The role of the RAD51D HR protein during thiopurine induction of mitotic catastrophe.
Title: Thiopurine-induced mitotic catastrophe in Rad51d-deficient mammalian cells.
RNF138 is a newly identified regulatory component of the HR mediated DNA repair pathway that has implications toward understanding how ubiquitination modifies the functions of the RAD51 paralog protein complex
Title: RNF138 interacts with RAD51D and is required for DNA interstrand crosslink repair and maintaining chromosome integrity.
Cells deficient in RAD51D (a RAD51 paralogue) are extremely sensitive to 6-thioguanine.
Title: The homologous recombination protein RAD51D mediates the processing of 6-thioguanine lesions downstream of mismatch repair.
RAD51D protects against MLH1-dependent cytotoxic responses to O(6)-methylguanine
Title: RAD51D protects against MLH1-dependent cytotoxic responses to O(6)-methylguanine.
Interactions of four candidate proteins (SFPQ, NONO, MSH2 and mini chromosome maintenance protein 2) were confirmed by Western blot analysis of co-precipitation eluates and were also verified to form ex vivo complexes with RAD51D.
Title: The interaction profile of homologous recombination repair proteins RAD51C, RAD51D and XRCC2 as determined by proteomic analysis.
Rad51d alternative splice variants potentially modulate mechanisms of homologous recombination by sequestering either RAD51C or XRCC2
Title: Functional characterization and identification of mouse Rad51d splice variants.
a fragment of Rad51B containing amino acid residues 1-75 interacts with the C-terminus and linker of Rad51C, residues 79-376, and this region of Rad51C also interacts with mRad51D and Xrcc3
Title: Domain mapping of the Rad51 paralog protein complexes.
These cell-based studies are the first evidence that ATP binding and hydrolysis by RAD51D are required for efficient HR repair of DNA interstrand crosslinks.
Title: The ATPase motif in RAD51D is required for resistance to DNA interstrand crosslinking agents and interaction with RAD51C.
Findings support a crucial role for mammalian RAD51D in normal development, recombination, and maintaining mammalian genome stability.
Title: Extensive chromosomal instability in Rad51d-deficient mouse cells.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (2)
Targeted (1) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Rad51l3 (e-PCR), detects polymorphism
- UniSTS:546563

Rad51l3 (e-PCR), detects polymorphism
- UniSTS:259456

248O2-T7 (e-PCR), detects polymorphism
- UniSTS:209555

D10Chm172 (e-PCR)
- UniSTS:518332

Rad51l3 (e-PCR), detects polymorphism
- UniSTS:144319

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP hydrolysis activity	IEA Inferred from Electronic Annotation more info
enables ATP-dependent DNA damage sensor activity	IEA Inferred from Electronic Annotation more info
enables ATP-dependent DNA damage sensor activity	ISO Inferred from Sequence Orthology more info
enables ATP-dependent activity, acting on DNA	IBA Inferred from Biological aspect of Ancestor more info
enables ATP-dependent activity, acting on DNA	ISO Inferred from Sequence Orthology more info
enables ATP-dependent activity, acting on DNA	ISS Inferred from Sequence or Structural Similarity more info
enables DNA binding	IEA Inferred from Electronic Annotation more info
contributes_to four-way junction DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables four-way junction DNA binding	IEA Inferred from Electronic Annotation more info
contributes_to four-way junction DNA binding	ISO Inferred from Sequence Orthology more info
enables gamma-tubulin binding	ISO Inferred from Sequence Orthology more info
enables gamma-tubulin binding	ISS Inferred from Sequence or Structural Similarity more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables single-stranded DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables single-stranded DNA binding	ISO Inferred from Sequence Orthology more info
enables single-stranded DNA binding	ISS Inferred from Sequence or Structural Similarity more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within DNA damage response	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in DNA recombination	IEA Inferred from Electronic Annotation more info
involved_in DNA repair	IEA Inferred from Electronic Annotation more info
involved_in DNA strand invasion	IBA Inferred from Biological aspect of Ancestor more info
involved_in DNA strand invasion	ISO Inferred from Sequence Orthology more info
involved_in DNA strand invasion	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within chromosome organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in double-strand break repair via homologous recombination	IBA Inferred from Biological aspect of Ancestor more info
involved_in double-strand break repair via homologous recombination	ISO Inferred from Sequence Orthology more info
involved_in double-strand break repair via homologous recombination	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within interstrand cross-link repair	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in reciprocal meiotic recombination	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within regulation of cell cycle	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in telomere maintenance	IBA Inferred from Biological aspect of Ancestor more info
involved_in telomere maintenance	IGI Inferred from Genetic Interaction more info	PubMed
involved_in telomere maintenance	ISO Inferred from Sequence Orthology more info
involved_in telomere maintenance	ISS Inferred from Sequence or Structural Similarity more info
involved_in telomere maintenance via recombination	ISO Inferred from Sequence Orthology more info
involved_in telomere maintenance via recombination	ISS Inferred from Sequence or Structural Similarity more info

Component	Evidence Code	Pubs
part_of Rad51B-Rad51C-Rad51D-XRCC2 complex	IBA Inferred from Biological aspect of Ancestor more info
part_of Rad51B-Rad51C-Rad51D-XRCC2 complex	ISO Inferred from Sequence Orthology more info
part_of Rad51B-Rad51C-Rad51D-XRCC2 complex	ISS Inferred from Sequence or Structural Similarity more info
located_in centrosome	ISO Inferred from Sequence Orthology more info
located_in centrosome	ISS Inferred from Sequence or Structural Similarity more info
located_in chromosome, telomeric region	IDA Inferred from Direct Assay more info	PubMed
located_in chromosome, telomeric region	ISO Inferred from Sequence Orthology more info
located_in chromosome, telomeric region	ISS Inferred from Sequence or Structural Similarity more info
is_active_in microtubule organizing center	IBA Inferred from Biological aspect of Ancestor more info
is_active_in replication fork	IBA Inferred from Biological aspect of Ancestor more info
located_in replication fork	ISO Inferred from Sequence Orthology more info
located_in replication fork	ISS Inferred from Sequence or Structural Similarity more info

General protein information

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Preferred Names: DNA repair protein RAD51 homolog 4

Names: RAD51 homolog D; RAD51-like 3; TRAD/RAD51L3

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001277938.1 → NP_001264867.1 DNA repair protein RAD51 homolog 4 isoform 2

Status: REVIEWED

Description

Transcript Variant: This variant (2, also known as Rad51d-7b or Trad-d2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1.

Source sequence(s)

AL603745, AL646089

UniProtKB/TrEMBL

Q9EP87

Conserved Domains (2) summary

TIGR02236
Location:10 → 300

recomb_radA; DNA repair and recombination protein RadA

cd01123
Location:82 → 313

Rad51_DMC1_radA; Rad51_DMC1_radA,B. This group of recombinases includes the eukaryotic proteins RAD51, RAD55/57 and the meiosis-specific protein DMC1, and the archaeal proteins radA and radB. They are closely related to the bacterial RecA group. Rad51 proteins catalyze a ...
NM_001277939.1 → NP_001264868.1 DNA repair protein RAD51 homolog 4 isoform 3

See identical proteins and their annotated locations for NP_001264868.1

Status: REVIEWED

Description

Transcript Variant: This variant (3, also known as Rad51d-delta10) contains an alternate 3' terminal exon, compared to variant 1. It encodes isoform 3 which is shorter and has a distinct C-terminus, compared to isoform 1.

Source sequence(s)

AL603745, AL646089

UniProtKB/TrEMBL

B5LBC4, Q9EQS6

Conserved Domains (2) summary

smart00382
Location:99 → 266

AAA; ATPases associated with a variety of cellular activities

cd01123
Location:82 → 302

Rad51_DMC1_radA; Rad51_DMC1_radA,B. This group of recombinases includes the eukaryotic proteins RAD51, RAD55/57 and the meiosis-specific protein DMC1, and the archaeal proteins radA and radB. They are closely related to the bacterial RecA group. Rad51 proteins catalyze a ...
NM_001277941.1 → NP_001264870.1 DNA repair protein RAD51 homolog 4 isoform 4

Status: REVIEWED

Description

Transcript Variant: This variant (4, also known as Rad51d-delta5 or Trad-d6) lacks an alternate in-frame exon in the 5' coding region compared to variant 1. It encodes isoform 4 which is shorter than isoform 1.

Source sequence(s)

AL603745, AL646089

Consensus CDS

CCDS70267.1

UniProtKB/TrEMBL

B1ARD4, Q9EPC0

Related

ENSMUSP00000090520.7, ENSMUST00000092844.13

Conserved Domains (2) summary

TIGR02236
Location:10 → 260

recomb_radA; DNA repair and recombination protein RadA

cl21455
Location:82 → 273

P-loop_NTPase; P-loop containing Nucleoside Triphosphate Hydrolases
NM_001277942.1 → NP_001264871.1 DNA repair protein RAD51 homolog 4 isoform 5

See identical proteins and their annotated locations for NP_001264871.1

Status: REVIEWED

Description

Transcript Variant: This variant (5, also known as Rad51d-delta7,8 or Trad-d3) lacks two alternate exons resulting in the loss of an in-frame segment in the 3' coding region compared to variant 1. It encodes isoform 5 which is shorter than isoform 1.

Source sequence(s)

AL603745, AL646089

Consensus CDS

CCDS70268.1

UniProtKB/TrEMBL

Q9EP85, Q9EP87

Related

ENSMUSP00000021033.10, ENSMUST00000021033.16

Conserved Domains (2) summary

cl21455
Location:82 → 264

P-loop_NTPase; P-loop containing Nucleoside Triphosphate Hydrolases

cl23768
Location:26 → 92

ENDO3c; endonuclease III; includes endonuclease III (DNA-(apurinic or apyrimidinic site) lyase), alkylbase DNA glycosidases (Alka-family) and other DNA glycosidases
NM_011235.4 → NP_035365.1 DNA repair protein RAD51 homolog 4 isoform 1

See identical proteins and their annotated locations for NP_035365.1

Status: REVIEWED

Description

Transcript Variant: This variant (1, also known as Rad51d-FL) encodes the longest isoform (1).

Source sequence(s)

AK147759, AL646089, CJ141615

Consensus CDS

CCDS36250.1

UniProtKB/Swiss-Prot

O55230

UniProtKB/TrEMBL

Q3UGT8, Q9EQS6

Related

ENSMUSP00000018985.9, ENSMUST00000018985.15

Conserved Domains (2) summary

TIGR02236
Location:10 → 305

recomb_radA; DNA repair and recombination protein RadA

cd01123
Location:82 → 318

Rad51_DMC1_radA; Rad51_DMC1_radA,B. This group of recombinases includes the eukaryotic proteins RAD51, RAD55/57 and the meiosis-specific protein DMC1, and the archaeal proteins radA and radB. They are closely related to the bacterial RecA group. Rad51 proteins catalyze a ...

RNA

NR_102717.1 RNA Sequence

Status: REVIEWED

Description

Transcript Variant: This variant (6, also known as Rad51d-delta8 or Trad-d1) lacks an alternate exon in the 3' region, compared to variant 1. This variant is represented as non-coding because the use of the 5'-most supported translational start codon, as used in variant 1, renders the transcript a candidate for nonsense-mediated mRNA decay (NMD).

Source sequence(s)

AL603745, AL646089
NR_102718.1 RNA Sequence

Status: REVIEWED

Description

Transcript Variant: This variant (7, also known as Rad51d-plus-int3 or Trad-d7) uses an alternate splice site in an internal exon, compared to variant 1. This variant is represented as non-coding because the use of the 5'-most supported translational start codon, as used in variant 1, renders the transcript a candidate for nonsense-mediated mRNA decay (NMD).

Source sequence(s)

AL603745, AL646089
NR_102719.1 RNA Sequence

Status: REVIEWED

Description

Transcript Variant: This variant (8, also known as Rad51d-delta3 or Trad-d4) lacks an alternate exon in the 5' region compared to variant 1. This variant is represented as non-coding because the use of the 5'-most supported translational start codon, as used in variant 1, renders the transcript a candidate for nonsense-mediated mRNA decay (NMD).

Source sequence(s)

AK036272, AL646089, CF746344, CJ141615
NR_102720.1 RNA Sequence

Status: REVIEWED

Description

Transcript Variant: This variant (9, also known as Rad51d-delta3,7b or Trad-d5) lacks an alternate exon in the 5' region and uses an alternate splice site in an internal exon, compared to variant 1. This variant is represented as non-coding because the use of the 5'-most supported translational start codon, as used in variant 1, renders the transcript a candidate for nonsense-mediated mRNA decay (NMD).

Source sequence(s)

AL603745, AL646089

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000077.7 Reference GRCm39 C57BL/6J

Range

82762786..82781571 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_036156455.1 → XP_036012348.1 DNA repair protein RAD51 homolog 4 isoform X1

UniProtKB/TrEMBL

Q9EQS6

Conserved Domains (2) summary

PRK09302
Location:73 → 126

PRK09302; circadian clock protein KaiC; Reviewed

cd19489
Location:106 → 311

Rad51D; RAD51D recombinase