Mid1 midline 1 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Mid1provided by RGD
Official Full Name: midline 1provided by RGD
Primary source: RGD:2640
See related: EnsemblRapid:ENSRNOG00000003613 AllianceGenome:RGD:2640
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: Fxy; Midline1
Summary: Predicted to enable several functions, including microtubule binding activity; protein homodimerization activity; and ubiquitin protein ligase binding activity. Predicted to be involved in positive regulation of stress-activated MAPK cascade; protein localization to microtubule; and regulation of microtubule cytoskeleton organization. Predicted to act upstream of or within negative regulation of microtubule depolymerization. Predicted to be located in Golgi apparatus; cytosol; and microtubule cytoskeleton. Predicted to be active in cytoplasm. Human ortholog(s) of this gene implicated in Opitz GBBB syndrome. Orthologous to human MID1 (midline 1). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Biased expression in Muscle (RPKM 71.3), Kidney (RPKM 61.8) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: Xq13

Exon count:: 14

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	X	NC_086039.1 (27678248..28053049, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	X	NC_051356.1 (24116674..24491205, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	X	NC_005120.4 (25458782..25839941, complement)

Chromosome X - NC_086039.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Submit: New GeneRIF Correction

General gene information

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Markers

PMC311143P1 (e-PCR)
- UniSTS:272896

DXRat85 (e-PCR), detects polymorphism
- UniSTS:228443

Mid1 (e-PCR), detects polymorphism
- UniSTS:259696

DXGot43 (e-PCR)
- UniSTS:113795

DXUIA1 (e-PCR)
- UniSTS:239337

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables enzyme binding	ISO Inferred from Sequence Orthology more info
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables microtubule binding	ISO Inferred from Sequence Orthology more info
enables phosphoprotein binding	ISO Inferred from Sequence Orthology more info
enables protein homodimerization activity	ISO Inferred from Sequence Orthology more info
enables transferase activity	IEA Inferred from Electronic Annotation more info
enables ubiquitin protein ligase binding	ISO Inferred from Sequence Orthology more info
enables zinc ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within negative regulation of microtubule depolymerization	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of stress-activated MAPK cascade	ISO Inferred from Sequence Orthology more info
involved_in protein localization to microtubule	ISO Inferred from Sequence Orthology more info
involved_in regulation of microtubule cytoskeleton organization	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
located_in Golgi apparatus	IEA Inferred from Electronic Annotation more info
located_in Golgi apparatus	ISO Inferred from Sequence Orthology more info
located_in centriolar satellite	IEA Inferred from Electronic Annotation more info
located_in centriolar satellite	ISO Inferred from Sequence Orthology more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytoplasmic microtubule	ISO Inferred from Sequence Orthology more info
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in microtubule	IEA Inferred from Electronic Annotation more info
located_in microtubule	ISO Inferred from Sequence Orthology more info
located_in microtubule cytoskeleton	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: E3 ubiquitin-protein ligase Midline-1

Names: Finger on X and Y (in rat only on X); RING finger protein Midline-1; RING-type E3 ubiquitin transferase Midline-1; midline 1 (Opitz/BBB syndrome); tripartite motif-containing protein 18

NP_075216.1

EC 2.3.2.27

XP_006256887.1

EC 2.3.2.27

XP_017457631.1

EC 2.3.2.27

XP_017457632.1

EC 2.3.2.27

XP_038955925.1

EC 2.3.2.27

XP_038955926.1

EC 2.3.2.27

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001431605.1 → NP_001418534.1 E3 ubiquitin-protein ligase Midline-1 isoform 1

Status: VALIDATED

Source sequence(s)

JAXUCZ010000021

UniProtKB/Swiss-Prot

P82458

UniProtKB/TrEMBL

A6K2D8
NM_001431606.1 → NP_001418535.1 E3 ubiquitin-protein ligase Midline-1 isoform 1

Status: VALIDATED

Source sequence(s)

JAXUCZ010000021

UniProtKB/Swiss-Prot

P82458

UniProtKB/TrEMBL

A6K2D8
NM_001431607.1 → NP_001418536.1 E3 ubiquitin-protein ligase Midline-1 isoform 1

Status: VALIDATED

Source sequence(s)

JAXUCZ010000021

UniProtKB/Swiss-Prot

P82458

UniProtKB/TrEMBL

A6K2D8
NM_001431608.1 → NP_001418537.1 E3 ubiquitin-protein ligase Midline-1 isoform 1

Status: VALIDATED

Source sequence(s)

JAXUCZ010000021

UniProtKB/Swiss-Prot

P82458

UniProtKB/TrEMBL

A6K2D8
NM_001431609.1 → NP_001418538.1 E3 ubiquitin-protein ligase Midline-1 isoform 2

Status: VALIDATED

Source sequence(s)

JAXUCZ010000021
NM_022927.2 → NP_075216.1 E3 ubiquitin-protein ligase Midline-1 isoform 1

See identical proteins and their annotated locations for NP_075216.1

Status: VALIDATED

Source sequence(s)

JAXUCZ010000021

UniProtKB/Swiss-Prot

P82458

UniProtKB/TrEMBL

A6K2D8

Related

ENSRNOP00000004873.5, ENSRNOT00000004873.8

Conserved Domains (7) summary

smart00502
Location:219 → 344

BBC; B-Box C-terminal domain

cd00063
Location:381 → 481

FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...

cd16753
Location:8 → 61

RING-HC_MID1; RING finger, HC subclass, found in midline-1 (MID1) and similar proteins

cd19822
Location:168 → 214

Bbox2_MID1_C-I; B-box-type 2 zinc finger found in midline-1 (MID1) and similar proteins

cd19836
Location:115 → 164

Bbox1_MID1_C-I; B-box-type 1 zinc finger found in midline-1 (MID1) and similar proteins

pfam18568
Location:323 → 374

COS; TRIM C-terminal subgroup One Signature domain

cl02614
Location:485 → 661

SPRY; SPRY domain

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086039.1 Reference GRCr8

Range

27678248..28053049 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_017602142.3 → XP_017457631.1 E3 ubiquitin-protein ligase Midline-1 isoform X1

Conserved Domains (7) summary

smart00502
Location:219 → 344

BBC; B-Box C-terminal domain

cd00063
Location:381 → 481

FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...

cd16753
Location:8 → 61

RING-HC_MID1; RING finger, HC subclass, found in midline-1 (MID1) and similar proteins

cd19822
Location:168 → 214

Bbox2_MID1_C-I; B-box-type 2 zinc finger found in midline-1 (MID1) and similar proteins

cd19836
Location:115 → 164

Bbox1_MID1_C-I; B-box-type 1 zinc finger found in midline-1 (MID1) and similar proteins

pfam18568
Location:323 → 374

COS; TRIM C-terminal subgroup One Signature domain

cl02614
Location:485 → 661

SPRY; SPRY domain
XM_039099997.2 → XP_038955925.1 E3 ubiquitin-protein ligase Midline-1 isoform X1

Conserved Domains (7) summary

smart00502
Location:219 → 344

BBC; B-Box C-terminal domain

cd00063
Location:381 → 481

FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...

cd16753
Location:8 → 61

RING-HC_MID1; RING finger, HC subclass, found in midline-1 (MID1) and similar proteins

cd19822
Location:168 → 214

Bbox2_MID1_C-I; B-box-type 2 zinc finger found in midline-1 (MID1) and similar proteins

cd19836
Location:115 → 164

Bbox1_MID1_C-I; B-box-type 1 zinc finger found in midline-1 (MID1) and similar proteins

pfam18568
Location:323 → 374

COS; TRIM C-terminal subgroup One Signature domain

cl02614
Location:485 → 661

SPRY; SPRY domain
XM_017602143.3 → XP_017457632.1 E3 ubiquitin-protein ligase Midline-1 isoform X1

Conserved Domains (7) summary

smart00502
Location:219 → 344

BBC; B-Box C-terminal domain

cd00063
Location:381 → 481

FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...

cd16753
Location:8 → 61

RING-HC_MID1; RING finger, HC subclass, found in midline-1 (MID1) and similar proteins

cd19822
Location:168 → 214

Bbox2_MID1_C-I; B-box-type 2 zinc finger found in midline-1 (MID1) and similar proteins

cd19836
Location:115 → 164

Bbox1_MID1_C-I; B-box-type 1 zinc finger found in midline-1 (MID1) and similar proteins

pfam18568
Location:323 → 374

COS; TRIM C-terminal subgroup One Signature domain

cl02614
Location:485 → 661

SPRY; SPRY domain
XM_039099998.1 → XP_038955926.1 E3 ubiquitin-protein ligase Midline-1 isoform X2

Related

ENSRNOP00000101804.1, ENSRNOT00000141079.1

Conserved Domains (6) summary

smart00502
Location:182 → 306

BBC; B-Box C-terminal domain

cd00063
Location:343 → 443

FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...

cd16753
Location:8 → 61

RING-HC_MID1; RING finger, HC subclass, found in midline-1 (MID1) and similar proteins

cd19836
Location:115 → 164

Bbox1_MID1_C-I; B-box-type 1 zinc finger found in midline-1 (MID1) and similar proteins

pfam18568
Location:285 → 336

COS; TRIM C-terminal subgroup One Signature domain

cl02614
Location:447 → 623

SPRY; SPRY domain
XM_006256825.5 → XP_006256887.1 E3 ubiquitin-protein ligase Midline-1 isoform X1

Conserved Domains (7) summary

smart00502
Location:219 → 344

BBC; B-Box C-terminal domain

cd00063
Location:381 → 481

FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...

cd16753
Location:8 → 61

RING-HC_MID1; RING finger, HC subclass, found in midline-1 (MID1) and similar proteins

cd19822
Location:168 → 214

Bbox2_MID1_C-I; B-box-type 2 zinc finger found in midline-1 (MID1) and similar proteins

cd19836
Location:115 → 164

Bbox1_MID1_C-I; B-box-type 1 zinc finger found in midline-1 (MID1) and similar proteins

pfam18568
Location:323 → 374

COS; TRIM C-terminal subgroup One Signature domain

cl02614
Location:485 → 661

SPRY; SPRY domain