MSH3 mismatch repair protein MSH3 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: MSH3
Official Full Name: mismatch repair protein MSH3
Primary source: SGD:S000000688
Locus tag: YCR092C
See related: AllianceGenome:SGD:S000000688; FungiDB:YCR092C; VEuPathDB:YCR092C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables DNA insertion or deletion binding activity and double-strand/single-strand DNA junction binding activity. Contributes to Y-form DNA binding activity and heteroduplex DNA loop binding activity. Involved in DNA metabolic process. Located in cytoplasm and nucleus. Part of MutSbeta complex. Used to study cancer. Human ortholog(s) of this gene implicated in colorectal carcinoma; endometrial cancer; endometrial carcinoma; familial adenomatous polyposis 4; and lung non-small cell carcinoma. Orthologous to human MSH3 (mutS homolog 3). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See MSH3 in Genome Data Viewer

Location:: chromosome: III

Exon count:: 1

Sequence:: Chromosome: III; NC_001135.5 (276764..279820, complement)

Chromosome III - NC_001135.5 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001135.5

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Elevated MSH2 MSH3 expression interferes with DNA metabolism in vivo.
Title: Elevated MSH2 MSH3 expression interferes with DNA metabolism in vivo.
Msh2-Msh3 hops over nucleosomes and other protein roadblocks, but maintains sufficient contact with DNA to recognize a single lesion.
Title: Dynamic DNA binding licenses a repair factor to bypass roadblocks in search of DNA lesions.
Trinucleotide Repeat (TNR) tracts were stabilized in the msh3Delta background, indicating that Msh2-Msh3 plays important role in shifting expansion-contraction equilibrium toward expansion in early stages of TNR tract expansion.
Title: MSH3 Promotes Dynamic Behavior of Trinucleotide Repeat Tracts In Vivo.
Data suggest that bot hATP and structure-specific repair substrates cooperate to direct Msh2-Msh3-mediated repair and provide an explanation for the msh3Y942A separation-of-function phenotype.
Title: ATP binding and hydrolysis by Saccharomyces cerevisiae Msh2-Msh3 are differentially modulated by mismatch and double-strand break repair DNA substrates.
a comparative study of Msh2-Msh3 and Msh2-Msh6 for mispair binding, sliding clamp formation, and Mlh1-Pms1 recruitment
Title: Mispair-specific recruitment of the Mlh1-Pms1 complex identifies repair substrates of the Saccharomyces cerevisiae Msh2-Msh3 complex.
Mlh1-Mlh3, a meiotic crossover and DNA mismatch repair factor, is a Msh2-Msh3-stimulated endonuclease.
Title: Mlh1-Mlh3, a meiotic crossover and DNA mismatch repair factor, is a Msh2-Msh3-stimulated endonuclease.
Authors identified a set of aromatic residues within the FLY motif of the predicted Msh3 nucleotide binding pocket that are essential for Msh2-Msh3-mediated mismatch repair but are largely dispensable for 3' non-homologous tail removal.
Title: Distinct requirements within the Msh3 nucleotide binding pocket for mismatch and double-strand break repair.
Msh2-Msh3 directly interferes with normal Okazaki fragment processing by flap endonuclease1 (Rad27) and DNA ligase I (Cdc9) in the presence of TNR sequences, thereby producing small, incremental expansion events
Title: Msh2-Msh3 interferes with Okazaki fragment processing to promote trinucleotide repeat expansions.
Data suggest that recognition of small insertion/deletion mispairs by Msh3, but not Msh2, requires more interaction with DNA conformations induced by small insertion/deletion mispairs than induced by large that are bent and strand separated.
Title: Functional studies and homology modeling of Msh2-Msh3 predict that mispair recognition involves DNA bending and strand separation.
Msh3-like behaviors beyond mispair specificity are not features controlled by the mispair-binding domain
Title: Chimeric Saccharomyces cerevisiae Msh6 protein with an Msh3 mispair-binding domain combines properties of both proteins.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP-dependent DNA damage sensor activity	IEA Inferred from Electronic Annotation more info
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA insertion or deletion binding	IDA Inferred from Direct Assay more info	PubMed
contributes_to Y-form DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables double-strand/single-strand DNA junction binding	IDA Inferred from Direct Assay more info	PubMed
enables double-stranded DNA binding	IBA Inferred from Biological aspect of Ancestor more info
contributes_to heteroduplex DNA loop binding	IDA Inferred from Direct Assay more info	PubMed
contributes_to mismatched DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables mismatched DNA binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in DNA metabolic process	IEA Inferred from Electronic Annotation more info
involved_in DNA recombination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in DNA repair	IEA Inferred from Electronic Annotation more info
involved_in meiotic mismatch repair	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mismatch repair	IBA Inferred from Biological aspect of Ancestor more info
involved_in mismatch repair	IEA Inferred from Electronic Annotation more info
involved_in mismatch repair	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic recombination	IBA Inferred from Biological aspect of Ancestor more info
involved_in mitotic recombination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in removal of nonhomologous ends	IGI Inferred from Genetic Interaction more info	PubMed
involved_in removal of nonhomologous ends	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in replication fork arrest	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of MutSbeta complex	IPI Inferred from Physical Interaction more info	PubMed
located_in cytoplasm	HDA more info	PubMed
located_in nucleus	HDA more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: mismatch repair protein MSH3

NP_010016.2

Mismatch repair protein; forms dimers with Msh2p that mediate repair of insertion or deletion mutations and removal of nonhomologous DNA ends, contains a PCNA (Pol30p) binding motif required for genome stability

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001135.5 Reference assembly

Range

276764..279820 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001178798.1 → NP_010016.2 TPA: mismatch repair protein MSH3 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_010016.2

Status: REVIEWED

UniProtKB/Swiss-Prot

A6ZTR3, D6VR92, P25336

UniProtKB/TrEMBL

N1P7Y1

Conserved Domains (1) summary

COG0249
Location:127 → 1015

MutS; DNA mismatch repair ATPase MutS [Replication, recombination and repair]