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    CWC21 U2-type spliceosomal complex subunit CWC21 [ Saccharomyces cerevisiae S288C ]

    Gene ID: 852093, updated on 9-Dec-2024

    Summary

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    Official Symbol
    CWC21
    Official Full Name
    U2-type spliceosomal complex subunit CWC21
    Primary source
    SGD:S000002890
    Locus tag
    YDR482C
    See related
    AllianceGenome:SGD:S000002890; FungiDB:YDR482C; VEuPathDB:YDR482C
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Summary
    Involved in mRNA splicing, via spliceosome. Part of U2-type spliceosomal complex. [provided by Alliance of Genome Resources, Dec 2024]
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    Genomic context

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    See CWC21 in Genome Data Viewer
    Location:
    chromosome: IV
    Exon count:
    1
    Sequence:
    Chromosome: IV; NC_001136.10 (1420431..1420838, complement)

    Chromosome IV - NC_001136.10Genomic Context describing neighboring genes Neighboring gene Dig2p Neighboring gene alkaline phosphatase PHO8 Neighboring gene alpha-1,2-mannosyltransferase KRE2 Neighboring gene Vps52p

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_001136.10

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Physical and genetic interactions of yeast Cwc21p, an ortholog of human SRm300/SRRM2, suggest a role at the catalytic center of the spliceosome. Grainger RJ, et al. RNA, 2009 Dec. PMID 19854871, Free PMC Article
    2. Cwc21p promotes the second step conformation of the spliceosome and modulates 3' splice site selection. Gautam A, et al. Nucleic Acids Res, 2015 Mar 31. PMID 25740649, Free PMC Article
    3. The histone variant H2A.Z promotes efficient cotranscriptional splicing in S. cerevisiae. Neves LT, et al. Genes Dev, 2017 Apr 1. PMID 28446598, Free PMC Article
    4. Decrypting the Information Exchange Pathways across the Spliceosome Machinery. Saltalamacchia A, et al. J Am Chem Soc, 2020 May 6. PMID 32275149, Free PMC Article
    5. A protein map of the yeast activated spliceosome as obtained by electron microscopy. Sun C, et al. RNA, 2016 Sep. PMID 27368340, Free PMC Article

    See all (44) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Microarray analysis shows changes in 3' splice site selection in introns in cwc21Delta cells. Propose role for Cwc21p positioning the 3' splice site at transition to the second step conformation of the spliceosome, mediated through interactions with U5 snRNP.
      Title: Cwc21p promotes the second step conformation of the spliceosome and modulates 3' splice site selection.
    2. The cwf21 domain in Cwc21p has been shown to bind directly to Prp8p
      Title: Physical and genetic interactions of yeast Cwc21p, an ortholog of human SRm300/SRRM2, suggest a role at the catalytic center of the spliceosome.
    3. results suggest multiple functions for Cwc21/SRm300 in the splicing process, including an important role in the activation of splicing in association with Isy1.
      Title: A systematic characterization of Cwc21, the yeast ortholog of the human spliceosomal protein SRm300.
    4. show that the N-terminus of Prp8p contains an intramolecular fold that is a Snu114p and Cwc21p interacting domain (SCwid).
      Title: Physical and genetic interactions of yeast Cwc21p, an ortholog of human SRm300/SRRM2, suggest a role at the catalytic center of the spliceosome.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    enables molecular_function ND
    No biological Data available
    more info
    		  
    Process Evidence Code Pubs
    involved_in RNA splicing IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in mRNA processing IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in mRNA splicing, via spliceosome IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in mRNA splicing, via spliceosome IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    part_of U2-type spliceosomal complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in nucleus IEA
    Inferred from Electronic Annotation
    more info
    		  
    part_of spliceosomal complex IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    U2-type spliceosomal complex subunit CWC21
    NP_010770.3
    • Protein involved in RNA splicing by the spliceosome; component of a complex containing Cef1p; interacts genetically with ISY1 and BUD13; may bind RNA; has similarity to S. pombe Cwf21p

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001136.10 Reference assembly

      Range
      1420431..1420838 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001180790.3 → NP_010770.3  TPA: U2-type spliceosomal complex subunit CWC21 [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_010770.3

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      D6VTA5, Q03375
      UniProtKB/TrEMBL
      B3LFI4, C7GKS1, C8Z640, G2WBL1, N1P7M0
      Conserved Domains (1) summary
      cl40451
      Location:62 → 109
      cwf21; cwf21 domain
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q03375.1

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