STU2 Stu2p [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: STU2
Official Full Name: Stu2p
Primary source: SGD:S000004035
Locus tag: YLR045C
See related: AllianceGenome:SGD:S000004035; FungiDB:YLR045C; VEuPathDB:YLR045C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables microtubule binding activity and microtubule plus end polymerase. Involved in microtubule polymerization and mitotic spindle organization. Acts upstream of or within microtubule nucleation. Located in cell cortex; kinetochore; and microtubule cytoskeleton. Orthologous to human CKAP5 (cytoskeleton associated protein 5). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See STU2 in Genome Data Viewer

Location:: chromosome: XII

Exon count:: 1

Sequence:: Chromosome: XII; NC_001144.5 (235037..237703, complement)

Chromosome XII - NC_001144.5 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001144.5

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The TOG protein Stu2 is regulated by acetylation.
Title: The TOG protein Stu2 is regulated by acetylation.
TUBA1A tubulinopathy mutants disrupt neuron morphogenesis and override XMAP215/Stu2 regulation of microtubule dynamics.
Title: TUBA1A tubulinopathy mutants disrupt neuron morphogenesis and override XMAP215/Stu2 regulation of microtubule dynamics.
Study identifies a Stu2 mutant that abolishes its kinetochore function and shows that it causes biorientation defects in vivo. Stu2-mediated pathway functions together with the Aurora B-mediated pathway. Altogether, work indicates that cells employ multiple pathways to ensure biorientation and the accuracy of chromosome segregation.
Title: Kinetochore-associated Stu2 promotes chromosome biorientation in vivo.
Findings raise the possibility that the microtubule polymerization activities mediated by Stu2p are regulated through sumoylation pathways.
Title: The TOG protein Stu2/XMAP215 interacts covalently and noncovalently with SUMO.
An unexpected antagonism has been found between the tubulin-binding Stu2 domains (TOGs) and the lattice-binding basic region: lattice binding by the basic region is weak when at least two TOGs engage tubulins, strong when TOGs are empty.
Title: Design principles of a microtubule polymerase.
TORC1/Kog1 signaling controls Stu2 localization and thereby contributes to proper microtubule cytoskeletal organization in interphase and mitosis.
Title: TORC1 signaling exerts spatial control over microtubule dynamics by promoting nuclear export of Stu2.
findings reveal an uncharacterized function of Stu2 that is regulated mechanically, such that kinetochore-microtubule attachments are intrinsically stabilized by tension, implicating it in the correction of erroneous kinetochore-microtubule attachments
Title: A TOG Protein Confers Tension Sensitivity to Kinetochore-Microtubule Attachments.
Stu2p/XMAP215 proteins are essential microtubule polymerases that use multiple alpha-beta-tubulin-interacting TOG domains to bind microtubule plus ends and catalyze fast microtubule growth.
Title: A tethered delivery mechanism explains the catalytic action of a microtubule polymerase.
Stu2 is a Saccharomyces cerevisiae microtubule polymerase.
Title: Stu2, the budding yeast XMAP215/Dis1 homolog, promotes assembly of yeast microtubules by increasing growth rate and decreasing catastrophe frequency.
anaphase Stu2-dependent microtubule dynamics are critical for separation of long chromosomes.
Title: Chromosome length and perinuclear attachment constrain resolution of DNA intertwines.

Submit: New GeneRIF Correction See all GeneRIFs (14)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables microtubule binding	IBA Inferred from Biological aspect of Ancestor more info
enables microtubule binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables microtubule plus end polymerase	IBA Inferred from Biological aspect of Ancestor more info
enables microtubule plus end polymerase	IDA Inferred from Direct Assay more info	PubMed
enables microtubule plus end polymerase	IEA Inferred from Electronic Annotation more info
enables microtubule plus-end binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in establishment or maintenance of microtubule cytoskeleton polarity	IBA Inferred from Biological aspect of Ancestor more info
involved_in establishment or maintenance of microtubule cytoskeleton polarity	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within microtubule nucleation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in microtubule polymerization	IBA Inferred from Biological aspect of Ancestor more info
involved_in microtubule polymerization	IDA Inferred from Direct Assay more info	PubMed
involved_in microtubule polymerization	IEA Inferred from Electronic Annotation more info
involved_in mitotic spindle organization	IBA Inferred from Biological aspect of Ancestor more info
involved_in mitotic spindle organization	IGI Inferred from Genetic Interaction more info	PubMed
involved_in spindle organization	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in cell cortex	IDA Inferred from Direct Assay more info	PubMed
located_in chromosome	IEA Inferred from Electronic Annotation more info
located_in chromosome, centromeric region	IEA Inferred from Electronic Annotation more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoskeleton	IEA Inferred from Electronic Annotation more info
located_in intracellular membraneless organelle	IEA Inferred from Electronic Annotation more info
is_active_in kinetochore	IBA Inferred from Biological aspect of Ancestor more info
located_in kinetochore	IDA Inferred from Direct Assay more info	PubMed
located_in kinetochore	IEA Inferred from Electronic Annotation more info
located_in microtubule	IEA Inferred from Electronic Annotation more info
colocalizes_with microtubule plus-end	IBA Inferred from Biological aspect of Ancestor more info
located_in spindle	IEA Inferred from Electronic Annotation more info
located_in spindle microtubule	IDA Inferred from Direct Assay more info	PubMed
is_active_in spindle pole	IBA Inferred from Biological aspect of Ancestor more info
is_active_in spindle pole body	IBA Inferred from Biological aspect of Ancestor more info
located_in spindle pole body	IDA Inferred from Direct Assay more info	PubMed
located_in spindle pole body	IEA Inferred from Electronic Annotation more info
located_in supramolecular complex	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: Stu2p

NP_013146.1

Microtubule polymerase of the XMAP215/Dis1 family; required for elongation of mitotic spindle during anaphase, as well as for spindle orientation and metaphase chromosome alignment; stabilizes or destabilizes kinetochore-microtubule attachment depending on kinetochore tension; interacts with spindle pole body component Spc72p; metazoan ch-TOG/XMAP215 family member

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001144.5 Reference assembly

Range

235037..237703 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001181932.1 → NP_013146.1 TPA: Stu2p [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_013146.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VY47, P46675

UniProtKB/TrEMBL

C8ZD17, G2WIN7

Conserved Domains (2) summary

COG1196
Location:658 → 760

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]

PTZ00108
Location:655 → 888

PTZ00108; DNA topoisomerase 2-like protein; Provisional