TLG2 Tlg2p [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: TLG2
Official Full Name: Tlg2p
Primary source: SGD:S000005378
Locus tag: YOL018C
See related: AllianceGenome:SGD:S000005378; FungiDB:YOL018C; VEuPathDB:YOL018C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables SNAP receptor activity. Involved in several processes, including Golgi to vacuole transport; mannosyl-inositol phosphorylceramide metabolic process; and protein transport. Located in endosome membrane and trans-Golgi network. Part of SNARE complex. Human ortholog(s) of this gene implicated in pseudohypoparathyroidism type IB. Orthologous to human STX16 (syntaxin 16). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See TLG2 in Genome Data Viewer

Location:: chromosome: XV

Exon count:: 1

Sequence:: Chromosome: XV; NC_001147.6 (290881..292074, complement)

Chromosome XV - NC_001147.6 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001147.6

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

secretion of enolase ENO2 is inhibited in a TLG2 knockout mutant.
Title: Tracing putative trafficking of the glycolytic enzyme enolase via SNARE-driven unconventional secretion.
Data show that the SM protein Vps45p, which regulates endosomal trafficking in yeast, binds the conserved N-terminal peptide of the syntaxin Tlg2p.
Title: The N-terminal peptide of the syntaxin Tlg2p modulates binding of its closed conformation to Vps45p.
Data support a model in which the Sec1p/Munc18 protein is required to facilitate a switch of Tlg2p and Sx16 from a closed to an open conformation, thus allowing SNARE-complex assembly and membrane fusion to proceed.
Title: Functional homology of mammalian syntaxin 16 and yeast Tlg2p reveals a conserved regulatory mechanism.
Disruption of this high-affinity binding between Vps45p and Tlg2p leads to downregulation of Tlg2p.
Title: Cellular levels of the syntaxin Tlg2p are regulated by a single mode of binding to Vps45p.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables SNAP receptor activity	IBA Inferred from Biological aspect of Ancestor more info
enables SNAP receptor activity	IDA Inferred from Direct Assay more info	PubMed
enables SNAP receptor activity	IEA Inferred from Electronic Annotation more info
enables SNARE binding	IBA Inferred from Biological aspect of Ancestor more info

Process	Evidence Code	Pubs
involved_in Golgi to vacuole transport	IGI Inferred from Genetic Interaction more info	PubMed
involved_in cytoplasm to vacuole targeting by the Cvt pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in endocytosis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in inositol phosphoceramide metabolic process	IGI Inferred from Genetic Interaction more info	PubMed
involved_in inositol phosphoceramide metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in intracellular protein transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in intracellular protein transport	IEA Inferred from Electronic Annotation more info
involved_in mannosyl-inositol phosphorylceramide metabolic process	IGI Inferred from Genetic Interaction more info	PubMed
involved_in mannosyl-inositol phosphorylceramide metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein exit from endoplasmic reticulum	IGI Inferred from Genetic Interaction more info	PubMed
involved_in protein secretion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein transport	IEA Inferred from Electronic Annotation more info
involved_in vesicle docking	IBA Inferred from Biological aspect of Ancestor more info
involved_in vesicle fusion	IBA Inferred from Biological aspect of Ancestor more info
involved_in vesicle fusion	IDA Inferred from Direct Assay more info	PubMed
involved_in vesicle-mediated transport	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in Golgi apparatus	IEA Inferred from Electronic Annotation more info
part_of SNARE complex	IBA Inferred from Biological aspect of Ancestor more info
part_of SNARE complex	IPI Inferred from Physical Interaction more info	PubMed
located_in cytosol	IEA Inferred from Electronic Annotation more info
is_active_in endomembrane system	IBA Inferred from Biological aspect of Ancestor more info
located_in endosome	IEA Inferred from Electronic Annotation more info
located_in endosome membrane	IDA Inferred from Direct Assay more info	PubMed
located_in endosome membrane	IEA Inferred from Electronic Annotation more info
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in trans-Golgi network	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: Tlg2p

NP_014624.1

Syntaxin-like t-SNARE; forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; required along with VPS45 for an early step of the constitutive CVT pathway; interactions with Vps45 prevents Tlg2p degradation, and facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16)

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001147.6 Reference assembly

Range

290881..292074 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001183272.1 → NP_014624.1 TPA: Tlg2p [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_014624.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W248, E9P933, Q08144

UniProtKB/TrEMBL

A6ZNI9, B3LJ39, B5VRN6, C7GQW4, C8ZHU6

Conserved Domains (1) summary

COG5325
Location:35 → 338

COG5325; t-SNARE complex subunit, syntaxin [Intracellular trafficking and secretion]