Rap1 Rap1 GTPase [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: Rap1provided by FlyBase
Official Full Name: Rap1 GTPaseprovided by FlyBase
Primary source: FLYBASE:FBgn0004636
Locus tag: Dmel_CG1956
See related: AllianceGenome:FB:FBgn0004636
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: BEST:GH18528; C-ras3; CG1956; D-Rap1; Dmel\CG1956; DRap; DRap1; Dras3; Dras62B; E(faf); EC3-7; FCG-C; l(3)62Bf; l(3)R; P08645; R; Rap; rap1; RAP1; ras 3; ras3; Ras3; RasIII
Summary: Enables GTPase activity and protein kinase binding activity. Involved in several processes, including compound eye morphogenesis; epithelial cell differentiation; and regulation of signal transduction. Located in cell leading edge; cytosol; and nucleus. Is expressed in several structures, including adult head; adult heart; adult ventral nerve cord; brain; and imaginal disc. Human ortholog(s) of this gene implicated in thrombocytopenia. Orthologous to several human genes including RAP1A (RAP1A, member of RAS oncogene family) and RAP1B (RAP1B, member of RAS oncogene family). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
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Genomic context

Location:: 62B7-62B7; 3-1.0 cM

Exon count:: 2

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	3L	NT_037436.4 (1859115..1862239, complement)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	3L	NT_037436.3 (1859115..1862239, complement)

Chromosome 3L - NT_037436.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The Rap1 small GTPase affects cell fate or survival and morphogenetic patterning during Drosophila melanogaster eye development.
Title: The Rap1 small GTPase affects cell fate or survival and morphogenetic patterning during Drosophila melanogaster eye development.
Rap1 promotes epithelial integrity and cell viability in a growing tissue.
Title: Rap1 promotes epithelial integrity and cell viability in a growing tissue.
show that Rap1 activity modulates Talin recruitment to sites of adhesion via its direct binding to Talin
Title: Direct binding of Talin to Rap1 is required for cell-ECM adhesion in Drosophila.
Rap1, Canoe and Mbt regulated epithelial morphogenesis by coupling stabilization of the adherens junction (AJ) protein E-Cadherin and Bazooka retention at the zonula adherens.
Title: Rap1, Canoe and Mbt cooperate with Bazooka to promote zonula adherens assembly in the fly photoreceptor.
the front/back polarity of border cells requires Rap1, hyperactivation of which disrupts cluster polarity and induces misoriented protrusions and loss of asymmetry in the actin network.
Title: Rap1 Negatively Regulates the Hippo Pathway to Polarize Directional Protrusions in Collective Cell Migration.
precisely regulated Rap1 activity reinforces connections between cells and polarizes the cluster, thus facilitating the coordinated collective migration of border cells.
Title: Rap1 GTPase promotes coordinated collective cell migration in vivo.
Rap1-interacting domains of Canoe mediate adherens junction and tricellular junction recruitment but are dispensable for membrane localization. Data also support a role for Canoe multimerization. These multifactorial inputs shape Canoe localization, correct Bazooka and adherens junction positioning, and thus apical-basal polarity.
Title: Rap1 acts via multiple mechanisms to position Canoe and adherens junctions and mediate apical-basal polarity establishment.
Gain-of-function studies using dominant-negative and constitutively active forms of Rap1 indicate that Rap1 contributes to axonal growth and guidance.
Title: The axon guidance function of Rap1 small GTPase is independent of PlexA RasGAP activity in Drosophila.
Rap1 is activated to its GTP-bound form in response to Hh ligand, and that the GTPase exchange factor C3G likely contributes to this activation.
Title: The small GTPase Rap1 is a modulator of Hedgehog signaling.
We show that Rap1 activity induces and/or maintains the formation of the apical domain of developing photoreceptor cells and that this event correlates with the apical accumulation and activity of SEV in presumptive R7 cells.
Title: Apical accumulation of the Sevenless receptor tyrosine kinase during Drosophila eye development is promoted by the small GTPase Rap1.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables GDP binding	IBA Inferred from Biological aspect of Ancestor more info
enables GTP binding	IBA Inferred from Biological aspect of Ancestor more info
enables GTP binding	IEA Inferred from Electronic Annotation more info
enables GTPase activity	IBA Inferred from Biological aspect of Ancestor more info
enables GTPase activity	IDA Inferred from Direct Assay more info	PubMed
enables GTPase activity	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in R7 cell fate commitment	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in Rap protein signal transduction	IBA Inferred from Biological aspect of Ancestor more info
involved_in Rap protein signal transduction	IDA Inferred from Direct Assay more info	PubMed
involved_in Rap protein signal transduction	IEA Inferred from Electronic Annotation more info
involved_in Rap protein signal transduction	IGI Inferred from Genetic Interaction more info	PubMed
involved_in adherens junction assembly	IGI Inferred from Genetic Interaction more info	PubMed
involved_in border follicle cell migration	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cellular response to cAMP	IBA Inferred from Biological aspect of Ancestor more info
involved_in dorsal closure	IGI Inferred from Genetic Interaction more info	PubMed
involved_in dorsal closure	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in germ-line stem cell population maintenance	IGI Inferred from Genetic Interaction more info	PubMed
involved_in hemocyte migration	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in imaginal disc-derived wing hair organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of hippo signaling	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of synaptic vesicle exocytosis	IBA Inferred from Biological aspect of Ancestor more info
involved_in ommatidial rotation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of ERK1 and ERK2 cascade	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of cell-cell adhesion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of sevenless signaling pathway	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of smoothened signaling pathway	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of smoothened signaling pathway	IGI Inferred from Genetic Interaction more info	PubMed
NOT involved_in regulation of JNK cascade	IGI Inferred from Genetic Interaction more info	PubMed
NOT involved_in regulation of JNK cascade	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of embryonic cell shape	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in substrate-dependent cell migration, cell extension	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in torso signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cell leading edge	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	HDA more info	PubMed
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: Rap1 GTPase

Names: CG1956-PA; CG1956-PB; CG1956-PC; Rap1-PA; Rap1-PB; Rap1-PC; enhancer of faf; roughened

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_037436.4 Reference assembly

Range

1859115..1862239 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_057509.5 → NP_476857.1 Rap1 GTPase, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_476857.1

Status: REVIEWED

UniProtKB/Swiss-Prot

P08645, Q2XYB6, Q540V3, Q9V3N0

UniProtKB/TrEMBL

M9MRT6

Related

FBpp0072746

Conserved Domains (1) summary

cd04175
Location:3 → 165

Rap1; Rap1 family GTPase consists of Rap1a and Rap1b isoforms
NM_001202094.2 → NP_001189023.1 Rap1 GTPase, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001189023.1

Status: REVIEWED

UniProtKB/Swiss-Prot

P08645, Q2XYB6, Q540V3, Q9V3N0

UniProtKB/TrEMBL

M9MRT6

Conserved Domains (1) summary

cd04175
Location:3 → 165

Rap1; Rap1 family GTPase consists of Rap1a and Rap1b isoforms
NM_001274366.1 → NP_001261295.1 Rap1 GTPase, isoform C [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001261295.1

Status: REVIEWED

UniProtKB/Swiss-Prot

P08645, Q2XYB6, Q540V3, Q9V3N0

UniProtKB/TrEMBL

M9MRT6

Conserved Domains (1) summary

cd04175
Location:3 → 165

Rap1; Rap1 family GTPase consists of Rap1a and Rap1b isoforms