TFG1 transcription factor IIF subunit TFG1 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: TFG1
Official Full Name: transcription factor IIF subunit TFG1
Primary source: SGD:S000003418
Locus tag: YGR186W
See related: AllianceGenome:SGD:S000003418; FungiDB:YGR186W; VEuPathDB:YGR186W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: RAP74; SSU71
Summary: Enables protein phosphatase activator activity. Involved in transcription elongation by RNA polymerase II and transcriptional start site selection at RNA polymerase II promoter. Part of transcription factor TFIIF complex. Orthologous to human GTF2F1 (general transcription factor IIF subunit 1). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See TFG1 in Genome Data Viewer

Location:: chromosome: VII

Exon count:: 1

Sequence:: Chromosome: VII; NC_001139.9 (867774..869981)

Chromosome VII - NC_001139.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001139.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Tfg1 C-terminal domain interacts with double-stranded DNA oligonucleotides.
Title: Structural and binding studies of the C-terminal domains of yeast TFIIF subunits Tfg1 and Tfg2.
Monitoring elongation complexes containing RNAPII and combinations of TFIIF (SSU71) and TFIIS to mechanical loads, found that both factors are independently capable of restoring arrested RNAPII to productive elongation.
Title: Transcription factors TFIIF and TFIIS promote transcript elongation by RNA polymerase II by synergistic and independent mechanisms.
TFIIF mainly prevents Pol II from pause entering, whereas TFIIS assists with pause recovery, and quantitatively described these effects on the kinetics of transcription elongation by Pol II.
Title: Transcription factors IIS and IIF enhance transcription efficiency by differentially modifying RNA polymerase pausing dynamics.
TFIIF stimulates formation of the first two phosphodiester bonds and dramatically stabilizes a short RNA-DNA hybrid in the RNA polymerase II (RNAPII) active center.
Title: Functions of Saccharomyces cerevisiae TFIIF during transcription start site utilization.
Evidence that the Tfg1/Tfg2 dimer interface of TFIIF is located in proximity to the B-finger of RNA polymerase II (TFIIB) near the active center of RNAP II where the TFIIB-TFIIF-RNAP II interface plays a key role in transcription start site selection.
Title: Evidence that the Tfg1/Tfg2 dimer interface of TFIIF lies near the active center of the RNA polymerase II initiation complex.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables RNA polymerase II general transcription initiation factor activity	IBA Inferred from Biological aspect of Ancestor more info
enables TFIIF-class transcription factor complex binding	IBA Inferred from Biological aspect of Ancestor more info
enables protein phosphatase activator activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in positive regulation of transcription elongation by RNA polymerase II	IEA Inferred from Electronic Annotation more info
involved_in transcription elongation by RNA polymerase II	IDA Inferred from Direct Assay more info	PubMed
involved_in transcription elongation by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in transcription initiation at RNA polymerase II promoter	IBA Inferred from Biological aspect of Ancestor more info
involved_in transcription initiation at RNA polymerase II promoter	IDA Inferred from Direct Assay more info	PubMed
involved_in transcription initiation at RNA polymerase II promoter	IEA Inferred from Electronic Annotation more info
involved_in transcription initiation at RNA polymerase II promoter	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in transcriptional start site selection at RNA polymerase II promoter	IGI Inferred from Genetic Interaction more info	PubMed
involved_in transcriptional start site selection at RNA polymerase II promoter	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in nucleus	IEA Inferred from Electronic Annotation more info
part_of transcription factor TFIIF complex	IBA Inferred from Biological aspect of Ancestor more info
part_of transcription factor TFIIF complex	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: transcription factor IIF subunit TFG1

NP_011702.1

TFIIF (Transcription Factor II) largest subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP74

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001139.9 Reference assembly

Range

867774..869981

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001181315.1 → NP_011702.1 TPA: transcription factor IIF subunit TFG1 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_011702.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VUW9, P41895

UniProtKB/TrEMBL

C7GVV5, N1P617

Conserved Domains (2) summary

PRK10856
Location:160 → 287

PRK10856; cytoskeleton protein RodZ

pfam05793
Location:318 → 580

TFIIF_alpha; Transcription initiation factor IIF, alpha subunit (TFIIF-alpha)