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    IST3 U2 snRNP complex subunit IST3 [ Saccharomyces cerevisiae S288C ]

    Gene ID: 854821, updated on 9-Dec-2024

    Summary

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    Official Symbol
    IST3
    Official Full Name
    U2 snRNP complex subunit IST3
    Primary source
    SGD:S000001444
    Locus tag
    YIR005W
    See related
    AllianceGenome:SGD:S000001444; FungiDB:YIR005W; VEuPathDB:YIR005W
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Also known as
    SNU17
    Summary
    Contributes to first spliceosomal transesterification activity. Involved in generation of catalytic spliceosome for first transesterification step; mRNA export from nucleus; and spliceosomal complex assembly. Part of RES complex and U2 snRNP. Orthologous to human RBMX2 (RNA binding motif protein X-linked 2). [provided by Alliance of Genome Resources, Dec 2024]
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    Genomic context

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    See IST3 in Genome Data Viewer
    Location:
    chromosome: IX
    Exon count:
    1
    Sequence:
    Chromosome: IX; NC_001141.2 (364889..365335)

    Chromosome IX - NC_001141.2Genomic Context describing neighboring genes Neighboring gene Aim21p Neighboring gene Djp1p Neighboring gene Pan1p Neighboring gene tRNA-Glu

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_001141.2

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. A novel yeast U2 snRNP protein, Snu17p, is required for the first catalytic step of splicing and for progression of spliceosome assembly. Gottschalk A, et al. Mol Cell Biol, 2001 May. PMID 11287609, Free PMC Article
    2. Structure of the yeast Pml1 splicing factor and its integration into the RES complex. Brooks MA, et al. Nucleic Acids Res, 2009 Jan. PMID 19033360, Free PMC Article
    3. An unusual RNA recognition motif acts as a scaffold for multiple proteins in the pre-mRNA retention and splicing complex. Trowitzsch S, et al. J Biol Chem, 2008 Nov 21. PMID 18809678
    4. A novel protein-protein interaction in the RES (REtention and Splicing) complex. Tripsianes K, et al. J Biol Chem, 2014 Oct 10. PMID 25160624, Free PMC Article
    5. Retention and splicing complex (RES) - the importance of cooperativity. Wysoczanski P, et al. RNA Biol, 2016. PMID 26479714, Free PMC Article

    See all (70) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. RES is a 71 kDa heterotrimer composed of the 3 proteins Pml1p, Bud13p and Snu17p. We solved the 3-dimensional structure of the core of the RES complex as well as the 2 dimers, Snu17p-Bud13p and Snu17p-Pml1p
      Title: Retention and splicing complex (RES) - the importance of cooperativity.
    2. An extended binding interface has been described between the Snu17p RNA recognition motif and Bud13p of the retention and splicing complex.
      Title: A novel protein-protein interaction in the RES (REtention and Splicing) complex.
    3. Snu17p RRM binds multiple ligand proteins and acts as a platform that displays functional modules of the ligands, including forkhead-associated domain of Pml1p and conserved polylysine motif of Bud13p
      Title: An unusual RNA recognition motif acts as a scaffold for multiple proteins in the pre-mRNA retention and splicing complex.
    4. It is shown that IST3, previously linked to splicing, is required for efficient processing of the MATa1 message, particularly the first intron.
      Title: Single-gene deletions that restore mating competence to diploid yeast.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    enables RNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    contributes_to first spliceosomal transesterification activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    contributes_to first spliceosomal transesterification activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in RNA splicing IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in generation of catalytic spliceosome for first transesterification step IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in mRNA export from nucleus IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in mRNA processing IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in mRNA splicing, via spliceosome IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in mRNA splicing, via spliceosome IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in mRNA splicing, via spliceosome IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in spliceosomal complex assembly IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    part_of RES complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of U2 snRNP IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of U2 snRNP IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in nucleus IEA
    Inferred from Electronic Annotation
    more info
    		  
    part_of precatalytic spliceosome IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of spliceosomal complex IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    U2 snRNP complex subunit IST3
    NP_012270.1
    • Component of the U2 snRNP; required for the first catalytic step of splicing and for spliceosomal assembly; interacts with Rds3p and is required for Mer1p-activated splicing; diploid mutants have a specific defect in MATa1 pre-mRNA splicing which leads to haploid gene expression in diploids

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001141.2 Reference assembly

      Range
      364889..365335
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001179527.1 → NP_012270.1  TPA: U2 snRNP complex subunit IST3 [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_012270.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      D6VVT5, P40565
      UniProtKB/TrEMBL
      A6ZVS3, B3LTK4, C7GJG7, C8ZAQ4, G2WGF1, N1P0Q4
      Conserved Domains (1) summary
      cd12411
      Location:22 → 109
      RRM_ist3_like; RNA recognition motif (RRM) found in ist3 family
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P40565.1

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