PUF3 mRNA-binding protein PUF3 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: PUF3
Official Full Name: mRNA-binding protein PUF3
Primary source: SGD:S000003936
Locus tag: YLL013C
See related: AllianceGenome:SGD:S000003936; FungiDB:YLL013C; VEuPathDB:YLL013C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables mRNA binding activity. Involved in several processes, including cellular response to glucose starvation; post-transcriptional regulation of gene expression; and regulation of ubiquinone biosynthetic process. Located in cytoplasmic side of mitochondrial outer membrane. Human ortholog(s) of this gene implicated in cerebellar ataxia type 47. Orthologous to several human genes including PUM2 (pumilio RNA binding family member 2). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See PUF3 in Genome Data Viewer

Location:: chromosome: XII

Exon count:: 1

Sequence:: Chromosome: XII; NC_001144.5 (122075..124714, complement)

Chromosome XII - NC_001144.5 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001144.5

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

A non-canonical Puf3p-binding sequence regulates CAT5/COQ7 mRNA under both fermentable and respiratory conditions in budding yeast.
Title: A non-canonical Puf3p-binding sequence regulates CAT5/COQ7 mRNA under both fermentable and respiratory conditions in budding yeast.
Yeast Puf3p-mediated mRNA decay is regulated by carbon source-specific differential interaction of Puf3p with Pop2p and Yak1p.
Title: Yeast Puf3p-mediated mRNA decay is regulated by carbon source-specific differential interaction of Puf3p with Pop2p and Yak1p.
Pbp1 associates with Puf3 and promotes translation of its target mRNAs involved in mitochondrial biogenesis.
Title: Pbp1 associates with Puf3 and promotes translation of its target mRNAs involved in mitochondrial biogenesis.
Fission Yeast PUF Proteins Puf3 and Puf4 Are Novel Regulators of PI4P5K Signaling.
Title: Fission Yeast PUF Proteins Puf3 and Puf4 Are Novel Regulators of PI4P5K Signaling.
Rpb4 and Puf3 imprint and post-transcriptionally control the stability of a common set of mRNAs in yeast.
Title: Rpb4 and Puf3 imprint and post-transcriptionally control the stability of a common set of mRNAs in yeast.
Puf3p represses a specific set of proteins involved in mitochondrial protein import, translation, and OxPhos complex assembly (pathways essential to prime mitochondrial biogenesis).
Title: Multi-omics Reveal Specific Targets of the RNA-Binding Protein Puf3p and Its Orchestration of Mitochondrial Biogenesis.
Data suggest that mRNA-binding protein PUF3 (Puf3p) may synchronize the translational efficiency (TE) of its targets to aid in the formation of specific protein complexes.
Title: Novel insights into global translational regulation through Pumilio family RNA-binding protein Puf3p revealed by ribosomal profiling.
multi-omics data point to multiple classes of Puf3p targets, which display coherent post-transcriptional regulatory properties and suggest Puf3p plays a broad, but nuanced, role in the fine-tuning of gene expression
Title: Integrated multi-omics analyses reveal the pleiotropic nature of the control of gene expression by Puf3p.
The results indicate a likely role Puf3p in the control of translation under oxidative stress conditions, linking its role in repression of protein synthesis to mitochondrially active genes.
Title: Puf3p induces translational repression of genes linked to oxidative stress.
Data show that Puf3p has a general repressive effect on mitochondrial OXPHOS abundance, translation, and respiration that does not involve changes in overall mitochondrial biogenesis and largely independent of TORC1-mitochondrial signaling.
Title: Repression of mitochondrial translation, respiration and a metabolic cycle-regulated gene, SLF1, by the yeast Pumilio-family protein Puf3p.

Submit: New GeneRIF Correction See all GeneRIFs (14)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables RNA binding	IEA Inferred from Electronic Annotation more info
enables mRNA 3'-UTR binding	IBA Inferred from Biological aspect of Ancestor more info
enables mRNA binding	HDA more info	PubMed
enables mRNA binding	IDA Inferred from Direct Assay more info	PubMed
enables mRNA binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in aerobic respiration	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cellular response to glucose starvation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in intracellular mRNA localization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitochondrion localization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitochondrion organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of gene expression	IEA Inferred from Electronic Annotation more info
involved_in nuclear-transcribed mRNA catabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay	IBA Inferred from Biological aspect of Ancestor more info
involved_in nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of translation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in post-transcriptional regulation of gene expression	IEA Inferred from Electronic Annotation more info
involved_in regulation of biological quality	IEA Inferred from Electronic Annotation more info
involved_in regulation of ubiquinone biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoplasmic side of mitochondrial outer membrane	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrial outer membrane	IEA Inferred from Electronic Annotation more info
located_in mitochondrion	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: mRNA-binding protein PUF3

NP_013088.1

Protein of the mitochondrial outer surface; links the Arp2/3 complex with the mitochondria during anterograde mitochondrial movement; also binds to and promotes degradation of mRNAs for select nuclear-encoded mitochondrial proteins; translationally represses oxidative stress-linked genes; regulates CoQ biosynthesis

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001144.5 Reference assembly

Range

122075..124714 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001181833.1 → NP_013088.1 TPA: mRNA-binding protein PUF3 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_013088.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VXY9, Q02601, Q07807

UniProtKB/TrEMBL

B5VMT1

Conserved Domains (1) summary

COG5099
Location:54 → 876

COG5099; RNA-binding protein of the Puf family, translational repressor [Translation, ribosomal structure and biogenesis]