RPS3 ribosomal 40S subunit protein S3 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: RPS3
Official Full Name: ribosomal 40S subunit protein S3
Primary source: SGD:S000005122
Locus tag: YNL178W
See related: AllianceGenome:SGD:S000005122; FungiDB:YNL178W; VEuPathDB:YNL178W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: SUF14
Summary: Enables DNA-(apurinic or apyrimidinic site) endonuclease activity. A structural constituent of ribosome. Involved in cytoplasmic translation; nonfunctional rRNA decay; and ribosomal small subunit export from nucleus. Acts upstream of or within maintenance of translational fidelity. Part of cytosolic small ribosomal subunit and preribosome, small subunit precursor. Orthologous to human RPS3 (ribosomal protein S3). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See RPS3 in Genome Data Viewer

Location:: chromosome: XIV

Exon count:: 1

Sequence:: Chromosome: XIV; NC_001146.8 (302680..303402)

Chromosome XIV - NC_001146.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001146.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Rps3 and eIF3 closely co-operate to control translation termination and stop codon readthrough.
Title: uS3/Rps3 controls fidelity of translation termination and programmed stop codon readthrough in co-operation with eIF3.
Mbf1 and Rps3 cooperate to maintain the reading frame of stalled ribosomes, while Asc1 also mediates distinct events that result in recruitment of the ribosome quality control complex and mRNA decay.
Title: Multi-protein bridging factor 1(Mbf1), Rps3 and Asc1 prevent stalled ribosomes from frameshifting.
binding of Yar1 to one N-domain and binding of Kap60 to the second N-domain of dimerized Rps3 orchestrates import and protection of the ribosomal protein
Title: Nuclear import of dimerized ribosomal protein Rps3 in complex with its chaperone Yar1.
identify Asc1p (aka RACK1) and Rps3p, both stable 40S subunit components, as additional 18S nonfunctional 18S rRNA decay factors
Title: ASC1 and RPS3: new actors in 18S nonfunctional rRNA decay.
Yar1 is a specific chaperone for Rps3, which serves to keep Rps3 soluble until its incorporation into the pre-ribosome
Title: Yar1 protects the ribosomal protein Rps3 from aggregation.
Rps3 is stoichiometrically modified by omega-monomethylation at arginine-146 and is associated with slow cell growth.
Title: Identification of methylated proteins in the yeast small ribosomal subunit: a role for SPOUT methyltransferases in protein arginine methylation.
Rps3p has a beta-lyase activity with a broad range of substrate specificity which cleaves phosphodiester bonds of UV or oxidatively damaged DNA substrates.
Title: Yeast ribosomal protein S3 possesses a β-lyase activity on damaged DNA.
Studies confirmed the promoter occupancy of RPS3 by Rap1p and other transcription factors Sfp1p, Hmo1p and Crf1p.
Title: Determination of the core promoter regions of the Saccharomyces cerevisiae RPS3 gene.
Overexpression suppresses stress sensitivity and ribosome biogenesis defect of yar1 mutants, but not ltv1 mutants
Title: Genetic and biochemical interactions among Yar1, Ltv1 and Rps3 define novel links between environmental stress and ribosome biogenesis in Saccharomyces cerevisiae.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables DNA-(apurinic or apyrimidinic site) endonuclease activity	IDA Inferred from Direct Assay more info	PubMed
enables RNA binding	IEA Inferred from Electronic Annotation more info
enables structural constituent of ribosome	IBA Inferred from Biological aspect of Ancestor more info
enables structural constituent of ribosome	IDA Inferred from Direct Assay more info	PubMed
enables structural constituent of ribosome	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in cytoplasmic translation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within maintenance of translational fidelity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nonfunctional rRNA decay	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in ribosomal small subunit export from nucleus	IGI Inferred from Genetic Interaction more info	PubMed
involved_in ribosomal subunit export from nucleus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in translation	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
part_of 90S preribosome	HDA more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytosolic ribosome	IEA Inferred from Electronic Annotation more info
part_of cytosolic small ribosomal subunit	IBA Inferred from Biological aspect of Ancestor more info
part_of cytosolic small ribosomal subunit	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
part_of preribosome, small subunit precursor	IDA Inferred from Direct Assay more info	PubMed
part_of ribonucleoprotein complex	IEA Inferred from Electronic Annotation more info
located_in ribosome	IEA Inferred from Electronic Annotation more info
part_of small ribosomal subunit	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: ribosomal 40S subunit protein S3

NP_014221.3

Protein component of the small (40S) ribosomal subunit; has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; nascent Rps3p is bound by specific chaperone Yar1p during translation; homologous to mammalian ribosomal protein S3 and bacterial S3

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001146.8 Reference assembly

Range

302680..303402

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001183016.3 → NP_014221.3 TPA: ribosomal 40S subunit protein S3 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_014221.3

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W107, P05750

UniProtKB/TrEMBL

A6ZRQ7, B3LP29, B5VQM9, C7GTF2, C8ZG50, G2WLT0, N1NXR8

Conserved Domains (1) summary

PTZ00084
Location:1 → 216

PTZ00084; 40S ribosomal protein S3; Provisional