RFC1 replication factor C subunit 1 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: RFC1
Official Full Name: replication factor C subunit 1
Primary source: SGD:S000005743
Locus tag: YOR217W
See related: AllianceGenome:SGD:S000005743; FungiDB:YOR217W; VEuPathDB:YOR217W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: CDC44
Summary: Contributes to DNA clamp loader activity. Involved in leading strand elongation; mismatch repair; and mitotic cell cycle. Part of DNA replication factor C complex. Human ortholog(s) of this gene implicated in West Nile fever and congenital heart disease. Orthologous to human RFC1 (replication factor C subunit 1). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See RFC1 in Genome Data Viewer

Location:: chromosome: XV

Exon count:: 1

Sequence:: Chromosome: XV; NC_001147.6 (749301..751886)

Chromosome XV - NC_001147.6 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001147.6

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

DNA polymerase epsilon outcompetes double-stranded DNA for recruiting Ctf18-RFC to the replisome.
Title: Structural Basis for the Recruitment of Ctf18-RFC to the Replisome.
Data show that ATP binding rates and ATP-dependent conformational changes in replication factor C (RFC) are fast relative to proliferating cell nuclear antigen (PCNA) binding rates.
Title: Kinetic analysis of PCNA clamp binding and release in the clamp loading reaction catalyzed by Saccharomyces cerevisiae replication factor C.
We show that Pol eta is less accurate than other yeast DNA polym and the accessory proteins have little effect on fidelity when bypassing a TT dimer. Accessory proteins participate in pol eta functions, but they do not suppress UV mutagenesis.
Title: Effects of accessory proteins on the bypass of a cis-syn thymine-thymine dimer by Saccharomyces cerevisiae DNA polymerase eta.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP hydrolysis activity	IEA Inferred from Electronic Annotation more info
enables DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables DNA binding	IEA Inferred from Electronic Annotation more info
contributes_to DNA clamp loader activity	IDA Inferred from Direct Assay more info	PubMed
enables DNA clamp loader activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in DNA repair	IEA Inferred from Electronic Annotation more info
involved_in DNA repair	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in DNA replication	IEA Inferred from Electronic Annotation more info
involved_in DNA strand elongation involved in DNA replication	IEA Inferred from Electronic Annotation more info
involved_in cell division	IEA Inferred from Electronic Annotation more info
involved_in leading strand elongation	IDA Inferred from Direct Assay more info	PubMed
involved_in mismatch repair	IGI Inferred from Genetic Interaction more info	PubMed
involved_in mitotic cell cycle	IGI Inferred from Genetic Interaction more info	PubMed
involved_in mitotic cell cycle	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of DNA replication factor C complex	IDA Inferred from Direct Assay more info	PubMed
part_of DNA replication factor C complex	IEA Inferred from Electronic Annotation more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: replication factor C subunit 1

NP_014860.1

Subunit of heteropentameric Replication factor C (RF-C); RF-C is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001147.6 Reference assembly

Range

749301..751886

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001183636.1 → NP_014860.1 TPA: replication factor C subunit 1 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_014860.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W2S3, P38630

UniProtKB/TrEMBL

B3LJP1, C7GWG5, C8ZGX2, N1NYN6

Conserved Domains (3) summary

COG5275
Location:1 → 277

COG5275; BRCT domain type II [General function prediction only]

PRK04195
Location:298 → 762

PRK04195; replication factor C large subunit; Provisional

pfam08519
Location:621 → 776

RFC1; Replication factor RFC1 C terminal domain