NFI1 SUMO ligase NFI1 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: NFI1
Official Full Name: SUMO ligase NFI1
Primary source: SGD:S000005682
Locus tag: YOR156C
See related: AllianceGenome:SGD:S000005682; FungiDB:YOR156C; VEuPathDB:YOR156C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: SIZ2
Summary: Enables SUMO transferase activity and double-stranded DNA binding activity. Involved in DNA double-strand break attachment to nuclear envelope; chromosome segregation; and protein sumoylation. Located in chromatin. Orthologous to several human genes including PIAS2 (protein inhibitor of activated STAT 2); PIAS3 (protein inhibitor of activated STAT 3); and PIAS4 (protein inhibitor of activated STAT 4). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See NFI1 in Genome Data Viewer

Location:: chromosome: XV

Exon count:: 1

Sequence:: Chromosome: XV; NC_001147.6 (628360..630540, complement)

Chromosome XV - NC_001147.6 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001147.6

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

DNA asymmetry promotes SUMO modification of the single-stranded DNA-binding protein RPA.
Title: DNA asymmetry promotes SUMO modification of the single-stranded DNA-binding protein RPA.
SUMOylation stabilizes sister kinetochore biorientation to allow timely anaphase.
Title: SUMOylation stabilizes sister kinetochore biorientation to allow timely anaphase.
The authors show that Siz2 is enriched at the replication fork barrier (RFB) in the ribosomal DNA (rDNA) and also controls the homeostasis of Tof2 protein. siz2Delta resulted in increased accumulation of total Tof2 in the cell and a consequent increase in the enrichment of Tof2 at the rDNA.
Title: Siz2 Prevents Ribosomal DNA Recombination by Modulating Levels of Tof2 in Saccharomyces cerevisiae.
double-strand break relocation to the nuclear envelope depends on SUMOylation mediated by the E3 ligases Siz2 and Mms21
Title: PolySUMOylation by Siz2 and Mms21 triggers relocation of DNA breaks to nuclear pores through the Slx5/Slx8 STUbL.
DNA break induction, the budding yeast SUMO ligase Siz2 collaborates with the ssDNA-binding complex RPA (replication protein A) to induce the sumoylation of recombination factors and confer damage resistance
Title: DNA break-induced sumoylation is enabled by collaboration between a SUMO ligase and the ssDNA-binding complex RPA.
The sumoylating activity of Siz2 at the nuclear periphery acts to maintain a balance of sumoylation that supports the localization of Sir2/Sir4 to these silent regions.
Title: The SUMO E3 ligase Siz2 exerts a locus-dependent effect on gene silencing in Saccharomyces cerevisiae.
The PIAS homologue Siz2 regulates perinuclear telomere position and telomerase activity in budding yeast.
Title: The PIAS homologue Siz2 regulates perinuclear telomere position and telomerase activity in budding yeast.
data suggest that SUMO attachment negatively regulates Flp1 levels, which may partially account for the increased 2 microm copy number in the siz1Delta siz2Delta strain
Title: Misregulation of 2 microm circle copy number in a SUMO pathway mutant.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables SUMO ligase activity	IBA Inferred from Biological aspect of Ancestor more info
enables SUMO transferase activity	IDA Inferred from Direct Assay more info	PubMed
enables SUMO transferase activity	IEA Inferred from Electronic Annotation more info
enables SUMO transferase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables double-stranded DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables double-stranded DNA binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables transferase activity	IEA Inferred from Electronic Annotation more info
enables zinc ion binding	IEA Inferred from Electronic Annotation more info
enables zinc ion binding	RCA inferred from Reviewed Computational Analysis more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA double-strand break attachment to nuclear envelope	IGI Inferred from Genetic Interaction more info	PubMed
involved_in chromosome segregation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein sumoylation	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein sumoylation	IDA Inferred from Direct Assay more info	PubMed
involved_in protein sumoylation	IEA Inferred from Electronic Annotation more info
involved_in protein sumoylation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in protein sumoylation	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in chromatin	IBA Inferred from Biological aspect of Ancestor more info
located_in chromatin	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	HDA more info	PubMed
located_in nucleus	HDA more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: SUMO ligase NFI1

NP_014799.1

SUMO E3 ligase; catalyzes sumoylation of Yku70p/80p and Sir4p promoting telomere anchoring to the nuclear envelope and regulating telomerase activity; DNA-bound form catalyzes a DNA-damaged triggered sumoylation wave resulting in multisite modification of several DNA repair proteins, enhancing interactions between these proteins and accelerating repair; sumoylates Cse4p, a prerequisite for STUbL-mediated Ub-dependent degradation; role in telomere length maintenance

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001147.6 Reference assembly

Range

628360..630540 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001183575.1 → NP_014799.1 TPA: SUMO ligase NFI1 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_014799.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W2L3, Q12216

UniProtKB/TrEMBL

C8ZGR4, N1NX17

Conserved Domains (4) summary

smart00513
Location:43 → 77

SAP; Putative DNA-binding (bihelical) motif predicted to be involved in chromosomal organisation

COG5422
Location:476 → 703

ROM1; RhoGEF, Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases [Signal transduction mechanisms]

cd16793
Location:335 → 382

SP-RING_ScSiz_like; SP-RING finger found in Saccharomyces cerevisiae E3 SUMO-protein ligase SIZ1, SIZ2, and similar proteins

pfam14324
Location:156 → 288

PINIT; PINIT domain