SKI8 SKI complex subunit WD repeat protein SKI8 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: SKI8
Official Full Name: SKI complex subunit WD repeat protein SKI8
Primary source: SGD:S000003181
Locus tag: YGL213C
See related: AllianceGenome:SGD:S000003181; FungiDB:YGL213C; VEuPathDB:YGL213C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: REC103
Summary: Involved in nuclear-transcribed mRNA catabolic process; protein-DNA complex assembly; and reciprocal meiotic recombination. Located in nuclear chromosome. Part of Ski complex. Is active in cytoplasm. Orthologous to human SKIC8 (SKI8 subunit of superkiller complex). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See SKI8 in Genome Data Viewer

Location:: chromosome: VII

Exon count:: 1

Sequence:: Chromosome: VII; NC_001139.9 (90054..91247, complement)

Chromosome VII - NC_001139.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001139.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Ski2, Ski3, and Ski8 assemble in a tetramer with 1:1:2 stoichiometry; 370 kDa core complex shows that Ski3 forms an array of 33 tetratricopeptide repeat motifs organized in N-terminal and C-terminal arms. The C-terminal arm of Ski3 and the two Ski8 subunits position the helicase core of Ski2 centrally within the complex, enhancing RNA binding.
Title: The yeast ski complex: crystal structure and RNA channeling to the exosome complex.
Ski8p has seven WD repeats, demonstrating that many WD repeats have not yet been identified in sequences and also raises the possibility that the seven-bladed propeller may be the predominant fold for this family of proteins
Title: The structure of Ski8p, a protein regulating mRNA degradation: Implications for WD protein structure.
Mapping sequence conservation and hydrophobicities of amino acids on the molecular surface of Ski8p reveals a prominent site on the top surface of the beta propeller, which is most likely involved in mediating interactions of Ski8p with Ski3p and Spo11p
Title: Crystal structure of Ski8p, a WD-repeat protein with dual roles in mRNA metabolism and meiotic recombination.
Ski3p serves as a scaffold protein with its C terminus interacting with Ski8p.
Title: Domain interactions within the Ski2/3/8 complex and between the Ski complex and Ski7p.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables molecular_function	ND No biological Data available more info

Process	Evidence Code	Pubs
involved_in defense response to virus	IEA Inferred from Electronic Annotation more info
involved_in meiotic cell cycle	IEA Inferred from Electronic Annotation more info
involved_in nuclear-transcribed mRNA catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay	IGI Inferred from Genetic Interaction more info	PubMed
involved_in nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nuclear-transcribed mRNA catabolic process, non-stop decay	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein-DNA complex assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein-containing complex assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in reciprocal meiotic recombination	IGI Inferred from Genetic Interaction more info	PubMed
involved_in reciprocal meiotic recombination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in reciprocal meiotic recombination	IPI Inferred from Physical Interaction more info	PubMed

Component	Evidence Code	Pubs
part_of Ski complex	IDA Inferred from Direct Assay more info	PubMed
part_of Ski complex	IPI Inferred from Physical Interaction more info	PubMed
located_in chromosome	IEA Inferred from Electronic Annotation more info
is_active_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in nuclear chromosome	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: SKI complex subunit WD repeat protein SKI8

NP_011302.1

Ski complex component and WD-repeat protein; mediates 3'-5' RNA degradation by the cytoplasmic exosome; also required for meiotic double-strand break recombination; null mutants have superkiller phenotype

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001139.9 Reference assembly

Range

90054..91247 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001181078.1 → NP_011302.1 TPA: SKI complex subunit WD repeat protein SKI8 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_011302.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VTU2, Q02793

UniProtKB/TrEMBL

B3LHQ9, C8Z842, N1P427

Conserved Domains (2) summary

sd00039
Location:19 → 60

7WD40; WD40 repeat [structural motif]

cl29593
Location:13 → 351

WD40; WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from ...