Aqp4 aquaporin 4 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Aqp4provided by RGD
Official Full Name: aquaporin 4provided by RGD
Primary source: RGD:2143
See related: EnsemblRapid:ENSRNOG00000016043 AllianceGenome:RGD:2143
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: Miwc; WCH4; AQP-4
Summary: This gene encodes a member of the aquaporin family of intrinsic membrane proteins that function as water-selective channels in the plasma membranes of many cells. This protein is the predominant aquaporin found in brain and has an important role in brain water homeostasis. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. A recent study provided evidence for translational readthrough in this gene and expression of an additional C-terminally extended isoform via the use of an alternative in-frame translation termination codon. [provided by RefSeq, Dec 2015]
Expression: Biased expression in Brain (RPKM 565.9), Muscle (RPKM 337.5) and 2 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 18p13

Exon count:: 8

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	18	NC_086036.1 (6782389..6799034, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	18	NC_051353.1 (6507903..6524558, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	18	NC_005117.4 (6766009..6782757, complement)

Chromosome 18 - NC_086036.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Inhibition of phosphodiesterase 4 attenuates aquaporin 4 expression and astrocyte swelling following cerebral ischemia/reperfusion injury.
Title: Inhibition of phosphodiesterase 4 attenuates aquaporin 4 expression and astrocyte swelling following cerebral ischemia/reperfusion injury.
AQP4 regulates ferroptosis and oxidative stress of Muller cells in diabetic retinopathy by regulating TRPV4.
Title: AQP4 regulates ferroptosis and oxidative stress of Muller cells in diabetic retinopathy by regulating TRPV4.
Inhibition of aquaporin-4 and its subcellular localization attenuates below-level central neuropathic pain by regulating astrocyte activation in a rat spinal cord injury model.
Title: Inhibition of aquaporin-4 and its subcellular localization attenuates below-level central neuropathic pain by regulating astrocyte activation in a rat spinal cord injury model.
Aquaporin 4 Mediates the Effect of Iron Overload on Hydrocephalus After Intraventricular Hemorrhage.
Title: Aquaporin 4 Mediates the Effect of Iron Overload on Hydrocephalus After Intraventricular Hemorrhage.
Reduction of claudin-5 and aquaporin-4 in the rat hippocampal CA-1 and CA-3 regions of a learned helplessness model of depression.
Title: Reduction of claudin-5 and aquaporin-4 in the rat hippocampal CA-1 and CA-3 regions of a learned helplessness model of depression.
Aquaporin-4 expression and modulation in a rat model of post-traumatic syringomyelia.
Title: Aquaporin-4 expression and modulation in a rat model of post-traumatic syringomyelia.
Aquaporin 4 Expression Level Is Decreased in Skeletal Muscles with Aging.
Title: Aquaporin 4 Expression Level Is Decreased in Skeletal Muscles with Aging.
High-intensity interval training ameliorates Alzheimer's disease-like pathology by regulating astrocyte phenotype-associated AQP4 polarization.
Title: High-intensity interval training ameliorates Alzheimer's disease-like pathology by regulating astrocyte phenotype-associated AQP4 polarization.
Upregulation of TRPC6 inhibits astrocyte activation and proliferation after spinal cord injury in rats by suppressing AQP4 expression.
Title: Upregulation of TRPC6 inhibits astrocyte activation and proliferation after spinal cord injury in rats by suppressing AQP4 expression.
Silencing of Long Noncoding RNA GAS5 Blocks Experimental Cerebral Ischemia-Reperfusion Injury by Restraining AQP4 Expression via the miR-1192/STAT5A Axis.
Title: Silencing of Long Noncoding RNA GAS5 Blocks Experimental Cerebral Ischemia-Reperfusion Injury by Restraining AQP4 Expression via the miR-1192/STAT5A Axis.

Submit: New GeneRIF Correction See all GeneRIFs (213)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RH142179 (e-PCR)
- UniSTS:231429

RH138222 (e-PCR)
- UniSTS:220309

Aqp4 (e-PCR), detects polymorphism
- UniSTS:209565

RH94727 (e-PCR)
- UniSTS:119974

Aqp4 (e-PCR), detects polymorphism
- UniSTS:530943

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables channel activity	IEA Inferred from Electronic Annotation more info
enables identical protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables water channel activity	IBA Inferred from Biological aspect of Ancestor more info
enables water channel activity	IDA Inferred from Direct Assay more info	PubMed
enables water channel activity	IEA Inferred from Electronic Annotation more info
enables water channel activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in carbon dioxide transport	IDA Inferred from Direct Assay more info	PubMed
involved_in cell-cell adhesion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cellular response to estradiol stimulus	IEP Inferred from Expression Pattern more info	PubMed
involved_in cellular response to glucose stimulus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cellular response to interleukin-1	IEP Inferred from Expression Pattern more info	PubMed
involved_in cellular response to interleukin-6	IEP Inferred from Expression Pattern more info	PubMed
involved_in cellular response to type II interferon	IEA Inferred from Electronic Annotation more info
involved_in cellular response to type II interferon	ISO Inferred from Sequence Orthology more info
involved_in cerebrospinal fluid circulation	ISO Inferred from Sequence Orthology more info
involved_in cerebrospinal fluid circulation	ISS Inferred from Sequence or Structural Similarity more info
involved_in cerebrospinal fluid secretion	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within establishment of localization in cell	ISO Inferred from Sequence Orthology more info
involved_in female pregnancy	IEP Inferred from Expression Pattern more info	PubMed
involved_in intracellular water homeostasis	IDA Inferred from Direct Assay more info	PubMed
involved_in intracellular water homeostasis	IEA Inferred from Electronic Annotation more info
involved_in intracellular water homeostasis	ISO Inferred from Sequence Orthology more info
involved_in intracellular water homeostasis	ISS Inferred from Sequence or Structural Similarity more info
involved_in multicellular organismal-level water homeostasis	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within multicellular organismal-level water homeostasis	ISO Inferred from Sequence Orthology more info
involved_in multicellular organismal-level water homeostasis	ISO Inferred from Sequence Orthology more info
involved_in multicellular organismal-level water homeostasis	ISS Inferred from Sequence or Structural Similarity more info
involved_in negative regulation of cell adhesion molecule production	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of interleukin-1 beta production	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of interleukin-6 production	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein homotetramerization	IDA Inferred from Direct Assay more info	PubMed
involved_in protein homotetramerization	IEA Inferred from Electronic Annotation more info
involved_in protein homotetramerization	ISO Inferred from Sequence Orthology more info
involved_in regulation of vascular endothelial growth factor production	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within renal water absorption	ISO Inferred from Sequence Orthology more info
involved_in response to glucocorticoid	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within sensory perception of sound	ISO Inferred from Sequence Orthology more info
involved_in transmembrane transport	IEA Inferred from Electronic Annotation more info
involved_in water transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in water transport	IDA Inferred from Direct Assay more info	PubMed
involved_in water transport	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within water transport	ISO Inferred from Sequence Orthology more info
involved_in water transport	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in T-tubule	IDA Inferred from Direct Assay more info	PubMed
located_in astrocyte end-foot	IDA Inferred from Direct Assay more info	PubMed
located_in astrocyte end-foot	ISO Inferred from Sequence Orthology more info
located_in basal plasma membrane	IDA Inferred from Direct Assay more info	PubMed
is_active_in basolateral plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in basolateral plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in basolateral plasma membrane	IEA Inferred from Electronic Annotation more info
located_in basolateral plasma membrane	ISO Inferred from Sequence Orthology more info
located_in cell projection	IEA Inferred from Electronic Annotation more info
located_in cell projection membrane	IDA Inferred from Direct Assay more info	PubMed
located_in cell-cell junction	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in endosome membrane	IEA Inferred from Electronic Annotation more info
located_in external side of plasma membrane	IEA Inferred from Electronic Annotation more info
located_in external side of plasma membrane	ISO Inferred from Sequence Orthology more info
located_in extracellular region	IEA Inferred from Electronic Annotation more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
part_of protein-containing complex	IDA Inferred from Direct Assay more info	PubMed
located_in sarcolemma	IDA Inferred from Direct Assay more info	PubMed
located_in sarcolemma	IEA Inferred from Electronic Annotation more info
located_in sarcolemma	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: aquaporin-4

Names: mercurial-insensitive water channel

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001142366.2 → NP_001135838.1 aquaporin-4 isoform M23

See identical proteins and their annotated locations for NP_001135838.1

Status: REVIEWED

Description

Transcript Variant: This variant (2, also known as AQP4c) contains an alternate 5' terminal exon, which causes translation initiation from an in-frame, downstream start codon, compared to variant 1. The resulting isoform (M23) has a shorter N-terminus compared to isoform M1.

Source sequence(s)

JAXUCZ010000018

UniProtKB/TrEMBL

A0A8L2R6N8

Related

ENSRNOP00000108306.1, ENSRNOT00000172814.1

Conserved Domains (1) summary

pfam00230
Location:9 → 226

MIP; Major intrinsic protein
NM_001270558.2 → NP_001257487.1 aquaporin-4 isoform 3

See identical proteins and their annotated locations for NP_001257487.1

Status: REVIEWED

Description

Transcript Variant: This variant (3, also known as AQP4b) lacks an in-frame coding exon compared to variant 1. The resulting isoform (3) is shorter, missing an internal protein segment compared to isoform M1.

Source sequence(s)

JAXUCZ010000018

UniProtKB/Swiss-Prot

P47863

Conserved Domains (1) summary

cl00200
Location:31 → 193

MIP; Major intrinsic protein (MIP) superfamily. Members of the MIP superfamily function as membrane channels that selectively transport water, small neutral molecules, and ions out of and between cells. The channel proteins share a common fold: the N-terminal ...
NM_001270559.2 → NP_001257488.1 aquaporin-4 isoform 4

See identical proteins and their annotated locations for NP_001257488.1

Status: REVIEWED

Description

Transcript Variant: This variant (4, also known as AQP4d) contains an alternate 5' terminal exon and lacks an in-frame coding exon compared to variant 1. These changes result in translation initiation from an in-frame, downstream start codon, and an isoform (4) with a shorter N-terminus and missing an internal protein segment compared to isoform M1.

Source sequence(s)

JAXUCZ010000018

UniProtKB/TrEMBL

A0A0H2UHZ1

Related

ENSRNOP00000050942.3, ENSRNOT00000048109.5

Conserved Domains (1) summary

cl00200
Location:9 → 171

MIP; Major intrinsic protein (MIP) superfamily. Members of the MIP superfamily function as membrane channels that selectively transport water, small neutral molecules, and ions out of and between cells. The channel proteins share a common fold: the N-terminal ...
NM_001317749.1 → NP_001304678.1 aquaporin-4 isoform M1x

Status: REVIEWED

Description

Transcript Variant: This variant (1, also known as AQP4a) represents the predominant transcript and encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (M1) results from translation termination at the upstream UGA stop codon, while the longer isoform (M1x) results from UGA stop codon readthrough to the downstream UAA termination codon. This RefSeq represents the longer, C-terminally extended isoform (M1x). As the UGA stop codon has been reported to specify several alternative amino acids (tryptophan, cysteine, arginine and serine), its location in the longer isoform is denoted by an 'X'.

Source sequence(s)

JAXUCZ010000018

UniProtKB/TrEMBL

A0A8L2R6N8

Conserved Domains (1) summary

pfam00230
Location:31 → 248

MIP; Major intrinsic protein
NM_012825.4 → NP_036957.1 aquaporin-4 isoform M1

See identical proteins and their annotated locations for NP_036957.1

Status: REVIEWED

Description

Transcript Variant: This variant (1, also known as AQP4a) represents the predominant transcript and encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (M1) results from translation termination at the upstream UGA stop codon, while the longer isoform (M1x) results from UGA stop codon readthrough to the downstream UAA termination codon. This RefSeq represents the shorter isoform (M1).

Source sequence(s)

JAXUCZ010000018

UniProtKB/Swiss-Prot

A0A0G2K4I1, P47863, Q8CIY8

UniProtKB/TrEMBL

A0A8L2R6N8, A6KLU9

Related

ENSRNOP00000073052.2, ENSRNOT00000077606.3

Conserved Domains (1) summary

pfam00230
Location:31 → 248

MIP; Major intrinsic protein

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086036.1 Reference GRCr8

Range

6782389..6799034 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_039096583.2 → XP_038952511.1 aquaporin-4 isoform X8

UniProtKB/TrEMBL

A0A8I5ZY68

Conserved Domains (1) summary

cl00200
Location:72 → 234

MIP; Major intrinsic protein (MIP) superfamily. Members of the MIP superfamily function as membrane channels that selectively transport water, small neutral molecules, and ions out of and between cells. The channel proteins share a common fold: the N-terminal ...
XM_039096581.2 → XP_038952509.1 aquaporin-4 isoform X3

UniProtKB/TrEMBL

A0A8I5ZY68

Related

ENSRNOP00000072488.1, ENSRNOT00000090320.3

Conserved Domains (1) summary

pfam00230
Location:72 → 289

MIP; Major intrinsic protein
XM_039096587.2 → XP_038952515.1 aquaporin-4 isoform X10

UniProtKB/TrEMBL

A0A8I5ZY68

Conserved Domains (1) summary

pfam00230
Location:9 → 226

MIP; Major intrinsic protein
XM_063277111.1 → XP_063133181.1 aquaporin-4 isoform X5
XM_063277112.1 → XP_063133182.1 aquaporin-4 isoform X6
XM_039096584.2 → XP_038952512.2 aquaporin-4 isoform X1
XM_063277109.1 → XP_063133179.1 aquaporin-4 isoform X2

UniProtKB/TrEMBL

A0A8L2UNG3

Related

ENSRNOP00000063720.2, ENSRNOT00000068150.5
XM_039096582.2 → XP_038952510.1 aquaporin-4 isoform X7

Conserved Domains (1) summary

cl00200
Location:72 → 272

MIP; Major intrinsic protein (MIP) superfamily. Members of the MIP superfamily function as membrane channels that selectively transport water, small neutral molecules, and ions out of and between cells. The channel proteins share a common fold: the N-terminal ...
XM_039096585.2 → XP_038952513.1 aquaporin-4 isoform X12

Conserved Domains (1) summary

cl00200
Location:72 → 273

MIP; Major intrinsic protein (MIP) superfamily. Members of the MIP superfamily function as membrane channels that selectively transport water, small neutral molecules, and ions out of and between cells. The channel proteins share a common fold: the N-terminal ...
XM_063277117.1 → XP_063133187.1 aquaporin-4 isoform X14
XM_063277116.1 → XP_063133186.1 aquaporin-4 isoform X13
XM_063277115.1 → XP_063133185.1 aquaporin-4 isoform X11
XM_063277110.1 → XP_063133180.1 aquaporin-4 isoform X4
XM_063277114.1 → XP_063133184.1 aquaporin-4 isoform X9