SLA1 cytoskeletal protein-binding protein SLA1 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: SLA1
Official Full Name: cytoskeletal protein-binding protein SLA1
Primary source: SGD:S000000103
Locus tag: YBL007C
See related: AllianceGenome:SGD:S000000103; FungiDB:YBL007C; VEuPathDB:YBL007C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables cargo adaptor activity and ubiquitin binding activity. Involved in actin cortical patch assembly; endocytosis; and negative regulation of Arp2/3 complex-mediated actin nucleation. Located in actin cortical patch and nucleus. Part of SLAC complex and actin cytoskeleton-regulatory complex. Orthologous to several human genes including EHD1 (EH domain containing 1); EHD2 (EH domain containing 2); and EHD3 (EH domain containing 3). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See SLA1 in Genome Data Viewer

Location:: chromosome: II

Exon count:: 1

Sequence:: Chromosome: II; NC_001134.8 (212632..216366, complement)

Chromosome II - NC_001134.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001134.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

that Scd5/PP1 is important for recycling Prk1 targets to initiate new rounds of endocytosis and provide new mechanistic information on the role of the Sla1 SR domain in regulating progression to the invagination/actin phase of endocytosis.
Title: Role of Scd5, a protein phosphatase-1 targeting protein, in phosphoregulation of Sla1 during endocytosis.
Vps1p functions in the organization of the actin cytoskeleton via interaction with Sla1p
Title: The yeast dynamin-related GTPase Vps1p functions in the organization of the actin cytoskeleton via interaction with Sla1p.
This study presents the NMR structure of the NPFxD-bound state of SHD1 and a model for the protein-ligand complex.
Title: Structure of Sla1p homology domain 1 and interaction with the NPFxD endocytic internalization motif.
This study describes the role of Lsb5p in membrane-trafficking events at the plasma membrane and also demonstrate for the first time an interaction of Arf3 with the endocytic machinery in yeast.
Title: Lsb5p interacts with actin regulators Sla1p and Las17p, ubiquitin and Arf3p to couple actin dynamics to membrane trafficking processes.
Results discuss the interaction of Sla1p with Lsb5p, and demonstrate a requirement for Arf3p expression in order to localize Lsb5p to the cell cortex.
Title: Interactions between Sla1p, Lsb5p and Arf3p in yeast endocytosis.
Internalization of Dnf1p from the plasma membrane uses an NPFXD endocytosis signal and its recognition by Sla1p.
Title: Yeast P4-ATPases Drs2p and Dnf1p are essential cargos of the NPFXD/Sla1p endocytic pathway.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables actin binding	IEA Inferred from Electronic Annotation more info
enables cargo adaptor activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables cytoskeletal protein binding	IEA Inferred from Electronic Annotation more info
enables identical protein binding	IEA Inferred from Electronic Annotation more info
enables protein-macromolecule adaptor activity	IEA Inferred from Electronic Annotation more info
enables ubiquitin binding	IDA Inferred from Direct Assay more info	PubMed
enables ubiquitin binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in actin cortical patch assembly	IBA Inferred from Biological aspect of Ancestor more info
involved_in actin cortical patch assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in endocytosis	IEA Inferred from Electronic Annotation more info
involved_in endocytosis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of Arp2/3 complex-mediated actin nucleation	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of Arp2/3 complex-mediated actin nucleation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of Arp2/3 complex-mediated actin nucleation	IPI Inferred from Physical Interaction more info	PubMed
involved_in regulation of actin filament polymerization	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
part_of SLAC complex	IDA Inferred from Direct Assay more info	PubMed
part_of SLAC complex	IPI Inferred from Physical Interaction more info	PubMed
located_in actin cortical patch	IDA Inferred from Direct Assay more info	PubMed
located_in actin cortical patch	IEA Inferred from Electronic Annotation more info
part_of actin cytoskeleton-regulatory complex	IDA Inferred from Direct Assay more info	PubMed
located_in cell cortex	IDA Inferred from Direct Assay more info	PubMed
located_in cellular bud neck	HDA more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoskeleton	IEA Inferred from Electronic Annotation more info
located_in endosome	IEA Inferred from Electronic Annotation more info
located_in endosome membrane	IEA Inferred from Electronic Annotation more info
located_in mating projection tip	HDA more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info
located_in nucleus	IMP Inferred from Mutant Phenotype more info	PubMed
located_in plasma membrane	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: cytoskeletal protein-binding protein SLA1

NP_009546.1

Cytoskeletal protein binding protein; required for assembly of the cortical actin cytoskeleton; interacts with proteins regulating actin dynamics and proteins required for endocytosis; found in the nucleus and cell cortex; has 3 SH3 domains

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001134.8 Reference assembly

Range

212632..216366 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001178247.1 → NP_009546.1 TPA: cytoskeletal protein-binding protein SLA1 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_009546.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VPZ3, P32790

UniProtKB/TrEMBL

G2W902, N1P9R8

Conserved Domains (7) summary

cd09532
Location:655 → 716

SAM_SLA1_fungal; SAM domain of SLA1 subfamily

cd11773
Location:7 → 64

SH3_Sla1p_1; First Src Homology 3 domain of the fungal endocytic adaptor protein Sla1p

cd11775
Location:356 → 412

SH3_Sla1p_3; Third Src Homology 3 domain of the fungal endocytic adaptor protein Sla1p

pfam03983
Location:489 → 554

SHD1; SLA1 homology domain 1, SHD1

pfam09606
Location:831 → 1201

Med15; ARC105 or Med15 subunit of Mediator complex non-fungal

cl17036
Location:73 → 127

SH3; Src Homology 3 domain superfamily

cl26091
Location:738 → 848

MotB_plug; Membrane MotB of proton-channel complex MotA/MotB