TIF4632 translation initiation factor eIF4G [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: TIF4632
Official Full Name: translation initiation factor eIF4G
Primary source: SGD:S000003017
Locus tag: YGL049C
See related: AllianceGenome:SGD:S000003017; FungiDB:YGL049C; VEuPathDB:YGL049C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables mRNA binding activity; translation initiation factor activity; and translation initiation factor binding activity. Involved in cellular response to glucose starvation; stress granule assembly; and translational initiation. Located in P-body and cytoplasmic stress granule. Part of eukaryotic translation initiation factor 4F complex. Human ortholog(s) of this gene implicated in late onset Parkinson's disease. Orthologous to several human genes including EIF4G2 (eukaryotic translation initiation factor 4 gamma 2). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See TIF4632 in Genome Data Viewer

Location:: chromosome: VII

Exon count:: 1

Sequence:: Chromosome: VII; NC_001139.9 (406860..409604, complement)

Chromosome VII - NC_001139.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001139.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The domains of yeast eIF4G, eIF4E and the cap fine-tune eIF4A activities through an intricate network of stimulatory and inhibitory effects.
Title: The domains of yeast eIF4G, eIF4E and the cap fine-tune eIF4A activities through an intricate network of stimulatory and inhibitory effects.
mRNA- and factor-driven dynamic variability controls eIF4F-cap recognition for translation initiation.
Title: mRNA- and factor-driven dynamic variability controls eIF4F-cap recognition for translation initiation.
eIF4G is retained on ribosomes elongating and terminating on short upstream ORFs to control reinitiation in yeast.
Title: eIF4G is retained on ribosomes elongating and terminating on short upstream ORFs to control reinitiation in yeast.
Crystal structures of a fungal eIF4E-eIF4G complex were determined. The canonical and non-canonical motifs in 4E-BPs and eIF4G contribute differently to the affinity for eIF4E. Highly specialized structural motifs do exist and serve to modulate the affinity of protein-protein interactions that regulate cap-dependent translation initiation in fungi.
Title: Structural motifs in eIF4G and 4E-BPs modulate their binding to eIF4E to regulate translation initiation in yeast.
depleting eukaryotic translation initiation factor 4G (eIF4G), the scaffold subunit of eukaryotic translation initiation factor 4F (eIF4F), preferentially impacts short mRNAs with strong closed-loop potential and unstructured 5' UTRs, exactly the opposite features associated with hyperdependence on the eIF4B/helicases.
Title: eIF4B stimulates translation of long mRNAs with structured 5' UTRs and low closed-loop potential but weak dependence on eIF4G.
These results reveal and characterize an unexpected interdependence between the two RNA helicases and eIF4G, and suggest that Ded1p is an integral part of eIF4F, the complex comprising eIF4G, eIF4A, and eIF4E.
Title: Coupling between the DEAD-box RNA helicases Ded1p and eIF4A.
Data show that that deletion of eIF4G1, but not eIF4G2, impairs growth and global translation initiation rates.
Title: Functional overlap between eIF4G isoforms in Saccharomyces cerevisiae.
involvement of yeast eIF4G in pre-mRNA metabolism; Tif4631p and Tif4632p interact efficiently with U1 snRNA; Tif4631p and Tif4632p associate with protein components of the splicing machinery, namely Snu71p and Prp11p.
Title: Interaction of yeast eIF4G with spliceosome components: implications in pre-mRNA processing events.
structure of the complex formed by yeast eIF4G's middle domain and full-length eIF4A at 2.6-A resolution
Title: Crystal structure of the yeast eIF4A-eIF4G complex: an RNA-helicase controlled by protein-protein interactions.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables ATPase activator activity	ISS Inferred from Sequence or Structural Similarity more info	PubMed
enables RNA binding	IEA Inferred from Electronic Annotation more info
enables mRNA binding	HDA more info	PubMed
enables mRNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables mRNA binding	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables translation initiation factor activity	IBA Inferred from Biological aspect of Ancestor more info
enables translation initiation factor activity	IDA Inferred from Direct Assay more info	PubMed
enables translation initiation factor activity	IEA Inferred from Electronic Annotation more info
enables translation initiation factor activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables translation initiation factor binding	IDA Inferred from Direct Assay more info	PubMed
enables translation initiation factor binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables translation initiation factor binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in cellular response to glucose starvation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of formation of translation preinitiation complex	ISS Inferred from Sequence or Structural Similarity more info	PubMed
involved_in regulation of translation	IEA Inferred from Electronic Annotation more info
involved_in stress granule assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in translation	IEA Inferred from Electronic Annotation more info
involved_in translational initiation	IBA Inferred from Biological aspect of Ancestor more info
involved_in translational initiation	IDA Inferred from Direct Assay more info	PubMed
involved_in translational initiation	IEA Inferred from Electronic Annotation more info
involved_in translational initiation	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in P-body	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoplasmic stress granule	HDA more info	PubMed
located_in cytoplasmic stress granule	IDA Inferred from Direct Assay more info	PubMed
part_of eukaryotic translation initiation factor 4F complex	IBA Inferred from Biological aspect of Ancestor more info
part_of eukaryotic translation initiation factor 4F complex	IMP Inferred from Mutant Phenotype more info	PubMed

General protein information

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Preferred Names: translation initiation factor eIF4G

NP_011466.1

Translation initiation factor eIF4G and scaffold protein; subunit of the mRNA cap-binding protein complex (eIF4F) with eIF4E (Cdc33p) and eIF4A (Tif1p/Tif2p); proposed to stimulate the ATPase and helicase activities of eIF4A synergistically with eIF4G based on sequence similarity with Tif4631p; proposed to be required with eIF4E for Ded1p-mediated stimulation of mRNA recruitment during 48S preinitiation complex (PIC) assembly; associated with Pab1p-mediated poly(A)-tail stimulated, translation

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001139.9 Reference assembly

Range

406860..409604 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001180914.1 → NP_011466.1 TPA: translation initiation factor eIF4G [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_011466.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VU91, P39936

UniProtKB/TrEMBL

A6ZUU8, C7GS26, G2WE47, N1P1X7

Conserved Domains (2) summary

pfam02854
Location:567 → 808

MIF4G; MIF4G domain

pfam12152
Location:381 → 440

eIF_4G1; Eukaryotic translation initiation factor 4G1