PAM16 import motor complex subunit PAM16 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: PAM16
Official Full Name: import motor complex subunit PAM16
Primary source: SGD:S000003640
Locus tag: YJL104W
See related: AllianceGenome:SGD:S000003640; FungiDB:YJL104W; VEuPathDB:YJL104W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: MIA1; TIM16
Summary: Enables protein domain specific binding activity. Involved in protein import into mitochondrial matrix. Located in mitochondrion. Part of PAM complex, Tim23 associated import motor. Used to study osteochondrodysplasia. Human ortholog(s) of this gene implicated in spondylometaphyseal dysplasia Megarbane-Dagher-Melike type. Orthologous to human PAM16 (presequence translocase associated motor 16). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See PAM16 in Genome Data Viewer

Location:: chromosome: X

Exon count:: 1

Sequence:: Chromosome: X; NC_001142.9 (227327..227776)

Chromosome X - NC_001142.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001142.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Coupling to Pam16 differentially controls the dual role of Pam18 in protein import and respiratory chain formation.
Title: Coupling to Pam16 differentially controls the dual role of Pam18 in protein import and respiratory chain formation.
The yeast magmas ortholog pam16 has an essential function in fermentative growth that involves sphingolipid metabolism.
Title: The yeast magmas ortholog pam16 has an essential function in fermentative growth that involves sphingolipid metabolism.
Analysis of a previously uncharacterized region of Pam16 revealed its requirement for formation of an active Pam18:Pam16 complex able to stimulate Hsp70's ATPase activity.
Title: Reevaluation of the role of the Pam18:Pam16 interaction in translocation of proteins by the mitochondrial Hsp70-based import motor.
These data suggest a model in which Tim44 serves as a scaffold for precise positioning of mtHsp70 and its cochaperone Pam18 at the translocon.
Title: Interaction of the J-protein heterodimer Pam18/Pam16 of the mitochondrial import motor with the translocon of the inner membrane.
binding of Tim44 to mHsp70 prevents the formation of a complex between the latter and Tim14/Pam18-Tim16/Pam16.
Title: The interplay between components of the mitochondrial protein translocation motor studied using purified components.
import into the matrix space of mitochondria requires association of the co-chaperones Tim16 and Tim14 with the TIM23 preprotein translocase
Title: Association of the Tim14.Tim16 subcomplex with the TIM23 translocase is crucial for function of the mitochondrial protein import motor.
Pam16's J-like domain strongly interacts with Pam18's J domain, leading to a productive interaction of Pam18 with mtHsp70 at the import channel.
Title: Role of Pam16's degenerate J domain in protein import across the mitochondrial inner membrane.
Tim14 forms a complex with the J-like protein Tim16. The crystal structure of this complex shows that the conserved domains of the two proteins have virtually identical folds but completely different surfaces enabling them to perform different functions.
Title: Structure and function of Tim14 and Tim16, the J and J-like components of the mitochondrial protein import motor.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables protein domain specific binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in protein import into mitochondrial matrix	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein import into mitochondrial matrix	IEA Inferred from Electronic Annotation more info
involved_in protein import into mitochondrial matrix	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein transport	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
part_of PAM complex, Tim23 associated import motor	IDA Inferred from Direct Assay more info	PubMed
part_of TIM23 mitochondrial import inner membrane translocase complex	IBA Inferred from Biological aspect of Ancestor more info
part_of TIM23 mitochondrial import inner membrane translocase complex	IDA Inferred from Direct Assay more info	PubMed
part_of TIM23 mitochondrial import inner membrane translocase complex	IEA Inferred from Electronic Annotation more info
located_in mitochondrial inner membrane	IEA Inferred from Electronic Annotation more info
located_in mitochondrion	HDA more info	PubMed
located_in mitochondrion	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: import motor complex subunit PAM16

NP_012431.1

Subunit of the import motor (PAM complex); the PAM complex is a component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); forms a 1:1 subcomplex with Pam18p and inhibits its cochaperone activity; contains a J-like domain