RAD18 E3 ubiquitin-protein ligase RAD18 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: RAD18
Official Full Name: E3 ubiquitin-protein ligase RAD18
Primary source: SGD:S000000662
Locus tag: YCR066W
See related: AllianceGenome:SGD:S000000662; FungiDB:YCR066W; VEuPathDB:YCR066W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables single-stranded DNA binding activity. Contributes to single-stranded DNA helicase activity. Involved in postreplication repair and protein monoubiquitination. Located in chromatin and nucleus. Part of Rad6-Rad18 complex. Orthologous to human RAD18 (RAD18 E3 ubiquitin protein ligase). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See RAD18 in Genome Data Viewer

Location:: chromosome: III

Exon count:: 1

Sequence:: Chromosome: III; NC_001135.5 (231500..232963)

Chromosome III - NC_001135.5 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001135.5

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The Zn-finger of Saccharomyces cerevisiae Rad18 and its adjacent region mediate interaction with Rad5.
Title: The Zn-finger of Saccharomyces cerevisiae Rad18 and its adjacent region mediate interaction with Rad5.
The Pol30-K196 residue plays a critical role in budding yeast DNA post-replication repair through interaction with Rad18.
Title: The Pol30-K196 residue plays a critical role in budding yeast DNA postreplication repair through interaction with Rad18.
following UV-induced damage, reactivation of Rad18, even after prolonged G2 arrest, restores viability and genome integrity.
Title: The preference for error-free or error-prone postreplication repair in Saccharomyces cerevisiae exposed to low-dose methyl methanesulfonate is cell cycle dependent.
The results imply that sumoylated PCNA is the physiological ubiquitylation target of budding yeast Rad18.
Title: A SUMO-interacting motif activates budding yeast ubiquitin ligase Rad18 towards SUMO-modified PCNA.
In this study we showed that DNA damage induced by hydrogen peroxide at the same mutagen doses causes significantly more mutations and lethal events in the rad18 mutant cells compared to control wild-type cells
Title: [RAD18 gene product of yeast Saccharomyces cerevisiae controls mutagenesis induced by hydrogen peroxide].
Rad17, a subunit of the PCNA-like 9-1-1 checkpoint clamp is ubiquitylated; however, in contrast to previous results, modification of Rad17 is found to be independent of DNA damage, the Rad6-Rad18 complex.
Title: Ubiquitylation of the 9-1-1 checkpoint clamp is independent of rad6-rad18 and DNA damage.
Rad6-Rad18 and Ubc13-Mms2-Rad5 act sequentially and mediate proliferating cell nuclear antigen poly-ubiquitylation by stepwise addition of ubiquitin monomers.
Title: Mechanistic analysis of PCNA poly-ubiquitylation by the ubiquitin protein ligases Rad18 and Rad5.
key role for the RAD6-RAD18-RAD5 error-free postreplication repair (RAD6 error-free PRR) pathway in promoting cell growth and survival, in response to chronic low-dose ultraviolet light
Title: RAD6-RAD18-RAD5-pathway-dependent tolerance to chronic low-dose ultraviolet light.
The interactions between Rad18, RPA and DNA and discuss their contribution to the activation of DNA damage bypass, was examined.
Title: Cooperation of replication protein A with the ubiquitin ligase Rad18 in DNA damage bypass.
Rad18 is required for the formation of X-shaped sister chromatid junctions (SCJs) at damaged replication forks through a process involving PCNA polyubiquitylation and the ubiquitin-conjugating enzymes Mms2 and Ubc13
Title: SUMOylation regulates Rad18-mediated template switch.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
contributes_to single-stranded DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables single-stranded DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables single-stranded DNA binding	IEA Inferred from Electronic Annotation more info
contributes_to single-stranded DNA helicase activity	IDA Inferred from Direct Assay more info	PubMed
enables transferase activity	IEA Inferred from Electronic Annotation more info
enables ubiquitin protein ligase activity	IEA Inferred from Electronic Annotation more info
enables zinc ion binding	RCA inferred from Reviewed Computational Analysis more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA damage response	IEA Inferred from Electronic Annotation more info
involved_in DNA duplex unwinding	IEA Inferred from Electronic Annotation more info
involved_in DNA repair	IEA Inferred from Electronic Annotation more info
involved_in error-free postreplication DNA repair	IGI Inferred from Genetic Interaction more info	PubMed
involved_in error-free translesion synthesis	IGI Inferred from Genetic Interaction more info	PubMed
involved_in error-prone translesion synthesis	IGI Inferred from Genetic Interaction more info	PubMed
involved_in postreplication repair	IBA Inferred from Biological aspect of Ancestor more info
involved_in postreplication repair	IEA Inferred from Electronic Annotation more info
involved_in protein monoubiquitination	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein monoubiquitination	IEA Inferred from Electronic Annotation more info
involved_in protein monoubiquitination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein ubiquitination	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
part_of Rad6-Rad18 complex	IBA Inferred from Biological aspect of Ancestor more info
part_of Rad6-Rad18 complex	IDA Inferred from Direct Assay more info	PubMed
located_in chromatin	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: E3 ubiquitin-protein ligase RAD18

NP_009992.1

EC 2.3.2.27

E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001135.5 Reference assembly

Range

231500..232963

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001178777.1 → NP_009992.1 TPA: E3 ubiquitin-protein ligase RAD18 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_009992.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VR69, P10862, Q58AT6

UniProtKB/TrEMBL

B3LUC4, C7GUX9, C8Z4F4

Conserved Domains (1) summary

COG5432
Location:1 → 470

RAD18; RING-finger-containing E3 ubiquitin ligase [Signal transduction mechanisms]