U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

Links from GEO Profiles

    • Showing Current items.

    comt comatose [ Drosophila melanogaster (fruit fly) ]

    Gene ID: 47091, updated on 9-Dec-2024

    Summary

    Go to the top of the page Help
    Official Symbol
    comtprovided by FlyBase
    Official Full Name
    comatoseprovided by FlyBase
    Primary source
    FLYBASE:FBgn0000346
    Locus tag
    Dmel_CG1618
    See related
    AllianceGenome:FB:FBgn0000346
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Drosophila melanogaster
    Lineage
    Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
    Also known as
    CG 1618; CG1618; com; Comt; Dmel\CG1618; DmNSF; dNSF; dNSF-1; dnsf1; dNsf1; dNSF1; Nsf; NSF; NSF 1; NSF-1; Nsf1; NSF1
    Summary
    Predicted to enable ATP hydrolysis activity. Involved in several processes, including Golgi to lysosome transport; chemical synaptic transmission; and modulation of chemical synaptic transmission. Located in cytoplasm; neuromuscular junction of somatic muscle; and terminal bouton. Is active in neuromuscular junction and presynaptic cytosol. Colocalizes with synaptic vesicle. Is expressed in several structures, including adult head; central brain primordium; ganglia; sensory nervous system primordium; and synaptic neuropil domain. Used to study Parkinson's disease and epilepsy. Human ortholog(s) of this gene implicated in developmental and epileptic encephalopathy 96. Orthologous to human NSF (N-ethylmaleimide sensitive factor, vesicle fusing ATPase). [provided by Alliance of Genome Resources, Dec 2024]
    Orthologs
    all
    NEW
    Try the new Gene table
    Try the new Transcript table

    Genomic context

    Go to the top of the page Help
    See comt in Genome Data Viewer
    Location:
    11E8-11E8; 1-42 cM
    Exon count:
    7
    Annotation release Status Assembly Chr Location
    Release 6.54 current Release 6 plus ISO1 MT (GCF_000001215.4) X NC_004354.4 (13259021..13262488)
    Release 5.57 previous assembly Release 5 (GCF_000001215.2) X NC_004354.3 (13153054..13156521)

    Chromosome X - NC_004354.4Genomic Context describing neighboring genes Neighboring gene multiple edematous wings Neighboring gene uncharacterized protein Neighboring gene uncharacterized protein Neighboring gene Histone deacetylase 4 Neighboring gene uncharacterized protein

    Genomic regions, transcripts, and products

    Go to the top of the page Help

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

    Go to the top of the pageHelp

    Related articles in PubMed

    1. A Drosophila NSF mutant. Pallanck L, et al. Nature, 1995 Jul 6. PMID 7596428
    2. SNAPs and NSF: general members of the fusion apparatus. Whiteheart SW, et al. Trends Cell Biol, 1995 Feb. PMID 14731414
    3. Lethal comatose mutation in Drosophila reveals possible role for NSF in neurogenesis. Sanyal S, et al. Neuroreport, 2001 May 25. PMID 11388412
    4. Courtship and other behaviors affected by a heat-sensitive, molecularly novel mutation in the cacophony calcium-channel gene of Drosophila. Chan B, et al. Genetics, 2002 Sep. PMID 12242229, Free PMC Article
    5. Phenotypic interaction between temperature-sensitive paralytic mutants comatose and paralytic suggests a role for N-ethylmaleimide-sensitive fusion factor in synaptic vesicle cycling in Drosophila. Sanyal S, et al. J Neurosci, 1999 Dec 15. PMID 10594091, Free PMC Article

    See all (118) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. The ubiquitin ligase Ariadne-1 regulates neurotransmitter release via ubiquitination of NSF.
      Title: The ubiquitin ligase Ariadne-1 regulates neurotransmitter release via ubiquitination of NSF.
    2. This study illustrated the comatose mutations in protein trafficking and synaptic vesicles function.
      Title: Genetic modifiers of comatose mutations in Drosophila: insights into neuronal NSF (N-ethylmaleimide-sensitive fusion factor) functions.
    3. Data show that NSF and SNAP exhibit activity-dependent binding to each other within living presynaptic terminals as well as distinctive interactions and mobilities.
      Title: Activity-dependent interactions of NSF and SNAP at living synapses.
    4. Results establish in vivo contributions of SNAP-25 and NSF to synaptic vesicle trafficking and define molecular mechanisms determining conserved functional properties of short-term depression.
      Title: Molecular mechanisms determining conserved properties of short-term synaptic depression revealed in NSF and SNAP-25 conditional mutants.
    5. Mutants Of NSF have been engineered that define key residues involved in two essential, but mechanistically distinct, biochemical functions of NSF: SNAP binding and SNAP-dependent ATPase stimulation.
      Title: Analysis of NSF mutants reveals residues involved in SNAP binding and ATPase stimulation.
    Submit: New GeneRIF Correction

    Pathways from PubChem

    Go to the top of the page

    Interactions

    Go to the top of the page Help
    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

    Go to the top of the page Help

    Homology

    Gene Ontology Provided by FlyBase

    Function Evidence Code Pubs
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ATP hydrolysis activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables ATP hydrolysis activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ATP hydrolysis activity ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    Process Evidence Code Pubs
    involved_in Golgi organization IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in Golgi to lysosome transport IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in Golgi to plasma membrane protein transport IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in SNARE complex disassembly IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in SNARE complex disassembly IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in autophagy IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in intra-Golgi vesicle-mediated transport IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in neuromuscular synaptic transmission IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    involved_in neuron cellular homeostasis IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in neurotransmitter secretion NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in regulation of neuromuscular synaptic transmission IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in regulation of short-term neuronal synaptic plasticity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in synaptic vesicle cycle IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in synaptic vesicle cycle IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    involved_in synaptic vesicle cycle IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in synaptic vesicle fusion to presynaptic active zone membrane IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in synaptic vesicle priming NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    Component Evidence Code Pubs
    is_active_in Golgi stack IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytoplasm IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cytoplasm NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    is_active_in neuromuscular junction IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in neuromuscular junction IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    located_in neuromuscular junction of somatic muscle IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in presynaptic cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in presynaptic cytosol IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    colocalizes_with synaptic vesicle IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in terminal bouton IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

    Go to the top of the page Help
    Preferred Names
    comatose
    Names
    CG1618-PA
    CG1618-PB
    N-Ethylmaleimide-sensitive factor
    N-ethyl-maleimide sensitive fusion protein
    N-ethylmaleimide-sensitive factor 1
    N-ethylmaleimide-sensitive fusion protein
    NEM-sensitive fusion protein
    comt-PA
    comt-PB

    NCBI Reference Sequences (RefSeq)

    Go to the top of the page Help

    NEW Try the new Transcript table

    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_004354.4 Reference assembly

      Range
      13259021..13262488
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_080138.4 → NP_524877.1  comatose, isoform A [Drosophila melanogaster]

      See identical proteins and their annotated locations for NP_524877.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      P46461, Q9VYF4
      UniProtKB/TrEMBL
      M9PH10
      Related
      FBpp0073585
      Conserved Domains (4) summary
      smart01072
      Location:111 → 158
      CDC48_2; Cell division protein 48 (CDC48) domain 2
      smart01073
      Location:5 → 83
      CDC48_N; Cell division protein 48 (CDC48) N-terminal domain
      COG0464
      Location:242 → 699
      SpoVK; AAA+-type ATPase, SpoVK/Ycf46/Vps4 family [Cell wall/membrane/envelope biogenesis, Cell cycle control, cell division, chromosome partitioning, Signal transduction mechanisms]
      pfam00004
      Location:256 → 397
      AAA; ATPase family associated with various cellular activities (AAA)

    2. NM_001272577.2 → NP_001259506.1  comatose, isoform B [Drosophila melanogaster]

      See identical proteins and their annotated locations for NP_001259506.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      P46461, Q9VYF4
      UniProtKB/TrEMBL
      M9PH10
      Conserved Domains (4) summary
      smart01072
      Location:111 → 158
      CDC48_2; Cell division protein 48 (CDC48) domain 2
      smart01073
      Location:5 → 83
      CDC48_N; Cell division protein 48 (CDC48) N-terminal domain
      COG0464
      Location:242 → 699
      SpoVK; AAA+-type ATPase, SpoVK/Ycf46/Vps4 family [Cell wall/membrane/envelope biogenesis, Cell cycle control, cell division, chromosome partitioning, Signal transduction mechanisms]
      pfam00004
      Location:256 → 397
      AAA; ATPase family associated with various cellular activities (AAA)
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    M9PH10 M9PH10
    P46461.1 P46461

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

    Other Literature Sources
    Chemical Information
    Molecular Biology Databases
    Research Materials
    Tools
    Miscellaneous