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    KAE1 tRNA N6-adenosine threonylcarbamoyltransferase [ Saccharomyces cerevisiae S288C ]

    Gene ID: 853910, updated on 9-Dec-2024

    Summary

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    Official Symbol
    KAE1
    Official Full Name
    tRNA N6-adenosine threonylcarbamoyltransferase
    Primary source
    SGD:S000001746
    Locus tag
    YKR038C
    See related
    AllianceGenome:SGD:S000001746; FungiDB:YKR038C; VEuPathDB:YKR038C
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Summary
    Enables chromatin DNA binding activity and nucleotidase activity. Involved in positive regulation of transcription by RNA polymerase II; tRNA metabolic process; and telomere maintenance via recombination. Located in chromatin. Part of EKC/KEOPS complex. Used to study primary hypomagnesemia and proteinuria. Human ortholog(s) of this gene implicated in Galloway-Mowat syndrome 3. Orthologous to human OSGEP (O-sialoglycoprotein endopeptidase). [provided by Alliance of Genome Resources, Dec 2024]
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    Genomic context

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    See KAE1 in Genome Data Viewer
    Location:
    chromosome: XI
    Exon count:
    1
    Sequence:
    Chromosome: XI; NC_001143.9 (511999..513159, complement)

    Chromosome XI - NC_001143.9Genomic Context describing neighboring genes Neighboring gene Caf4p Neighboring gene Spc34p Neighboring gene tRNA-Asp Neighboring gene amino acid permease GAP1

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_001143.9

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. tRNA N6-adenosine threonylcarbamoyltransferase defect due to KAE1/TCS3 (OSGEP) mutation manifest by neurodegeneration and renal tubulopathy. Edvardson S, et al. Eur J Hum Genet, 2017 May. PMID 28272532, Free PMC Article
    2. A role for the universal Kae1/Qri7/YgjD (COG0533) family in tRNA modification. El Yacoubi B, et al. EMBO J, 2011 Mar 2. PMID 21285948, Free PMC Article
    3. Kae1 of Saccharomyces cerevisiae KEOPS complex possesses ADP/GDP nucleotidase activity. Li QX, et al. Biochem J, 2022 Dec 9. PMID 36416748
    4. In vitro biosynthesis of a universal t6A tRNA modification in Archaea and Eukarya. Perrochia L, et al. Nucleic Acids Res, 2013 Feb 1. PMID 23258706, Free PMC Article
    5. Crystal structures of the Gon7/Pcc1 and Bud32/Cgi121 complexes provide a model for the complete yeast KEOPS complex. Zhang W, et al. Nucleic Acids Res, 2015 Mar 31. PMID 25735745, Free PMC Article

    See all (51) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. EKC/KEOPS complex is specifically involved in the regulation of Bud9p localization downstream of Rax1p/Rax2p.
      Title: Cell polarity in Saccharomyces cerevisiae depends on proper localization of the Bud9 landmark protein by the EKC/KEOPS complex.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    enables N(6)-L-threonylcarbamoyladenine synthase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables acyltransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables acyltransferase activity, transferring groups other than amino-acyl groups IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables chromatin DNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables nucleotidase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables nucleotidase activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables zinc ion binding RCA
    inferred from Reviewed Computational Analysis
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in chromosome organization IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in positive regulation of transcription by RNA polymerase II IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    involved_in tRNA modification IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in tRNA modification IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in tRNA processing IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in tRNA threonylcarbamoyladenosine metabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in tRNA threonylcarbamoyladenosine metabolic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in tRNA threonylcarbamoyladenosine modification IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in telomere maintenance IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in telomere maintenance IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    involved_in telomere maintenance via recombination IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in telomere maintenance via recombination IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    part_of EKC/KEOPS complex IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of EKC/KEOPS complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of EKC/KEOPS complex IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in chromatin IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cytoplasm HDA PubMed 
    is_active_in cytoplasm IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in nucleus HDA PubMed 
    located_in nucleus IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    tRNA N6-adenosine threonylcarbamoyltransferase
    NP_012964.2
    • ADP/GDP nucleotidase, essential functional component of KEOPS complex, with Bud32p, Cgi121p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and telomeric TG1-3 recombination; may have role in transcription; homolog of human OSGEP

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001143.9 Reference assembly

      Range
      511999..513159 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001179828.1 → NP_012964.2  TPA: tRNA N6-adenosine threonylcarbamoyltransferase [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_012964.2

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      D6VXA1, P36132
      UniProtKB/TrEMBL
      A7A000, B3LRB9, C7GNM4, C8ZCK3, G2WI68, N1P0H7
      Conserved Domains (1) summary
      PTZ00340
      Location:17 → 386
      PTZ00340; O-sialoglycoprotein endopeptidase-like protein; Provisional
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P36132.2

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