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    AFG2 AAA family ATPase AFG2 [ Saccharomyces cerevisiae S288C ]

    Gene ID: 851113, updated on 9-Dec-2024

    Summary

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    Official Symbol
    AFG2
    Official Full Name
    AAA family ATPase AFG2
    Primary source
    SGD:S000004389
    Locus tag
    YLR397C
    See related
    AllianceGenome:SGD:S000004389; FungiDB:YLR397C; VEuPathDB:YLR397C
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Also known as
    DRG1
    Summary
    Enables ATP hydrolysis activity. Involved in response to xenobiotic stimulus and ribosomal large subunit biogenesis. Part of preribosome, large subunit precursor. Orthologous to human AFG2A (AFG2 AAA ATPase homolog A). [provided by Alliance of Genome Resources, Dec 2024]
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    Genomic context

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    See AFG2 in Genome Data Viewer
    Location:
    chromosome: XII
    Exon count:
    1
    Sequence:
    Chromosome: XII; NC_001144.5 (912550..914892, complement)

    Chromosome XII - NC_001144.5Genomic Context describing neighboring genes Neighboring gene cytochrome c oxidase subunit VIII Neighboring gene tethering complex ATP-binding subunit VPS33 Neighboring gene SKI complex RNA helicase subunit SKI2 Neighboring gene chromatin-binding protein BDF1

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_001144.5

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. AFG2, an essential gene in yeast, encodes a new member of the Sec18p, Pas1p, Cdc48p, TBP-1 family of putative ATPases. Thorsness PE, et al. Yeast, 1993 Nov. PMID 8109176
    2. Structural and enzymatic properties of the AAA protein Drg1p from Saccharomyces cerevisiae. Decoupling of intracellular function from ATPase activity and hexamerization. Zakalskiy A, et al. J Biol Chem, 2002 Jul 26. PMID 12006565
    3. Structural basis for inhibition of the AAA-ATPase Drg1 by diazaborine. Prattes M, et al. Nat Commun, 2021 Jun 9. PMID 34108481, Free PMC Article
    4. Diazaborine resistance in the yeast Saccharomyces cerevisiae reveals a link between YAP1 and the pleiotropic drug resistance genes PDR1 and PDR3. Wendler F, et al. J Biol Chem, 1997 Oct 24. PMID 9341149
    5. Diazaborine treatment of Baker's yeast results in stabilization of aberrant mRNAs. Jungwirth H, et al. J Biol Chem, 2001 Sep 28. PMID 11477081

    See all (57) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Visualizing maturation factor extraction from the nascent ribosome by the AAA-ATPase Drg1.
      Title: Visualizing maturation factor extraction from the nascent ribosome by the AAA-ATPase Drg1.
    2. Structural basis for inhibition of the AAA-ATPase Drg1 by diazaborine.
      Title: Structural basis for inhibition of the AAA-ATPase Drg1 by diazaborine.
    3. the AAA-ATPase Drg1 as a target of diazaborine. Inhibitor binding into the second AAA domain of Drg1 requires ATP loading and results in inhibition of ATP hydrolysis in this site.
      Title: The drug diazaborine blocks ribosome biogenesis by inhibiting the AAA-ATPase Drg1.
    4. Data suggest that the ATPase activity of Drg1 is required for the release of shuttling proteins from the pre-60S particles shortly after their nuclear export.
      Title: Cytoplasmic recycling of 60S preribosomal factors depends on the AAA protein Drg1.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ATP hydrolysis activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables ATP hydrolysis activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables ATP hydrolysis activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ATP-dependent H2AZ histone chaperone activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ATP-dependent H3-H4 histone complex chaperone activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA clamp loader activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables chromatin extrusion motor activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables cohesin loader activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables hydrolase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in chromatin looping IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in chromatin remodeling IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in response to xenobiotic stimulus IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in ribosomal large subunit biogenesis IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in ribosomal large subunit biogenesis IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in ribosome biogenesis IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    is_active_in cytoplasm IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    part_of preribosome, large subunit precursor IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of preribosome, large subunit precursor IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    AAA family ATPase AFG2
    NP_013501.1
    • ATPase of the CDC48/PAS1/SEC18 (AAA) family, forms a hexameric complex; is essential for pre-60S maturation and release of several preribosome maturation factors; releases Rlp24p from purified pre-60S particles in vitro; target of the ribosomal biosynthesis inhibitor diazaborine; may be involved in degradation of aberrant mRNAs

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001144.5 Reference assembly

      Range
      912550..914892 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001182285.1 → NP_013501.1  TPA: AAA family ATPase AFG2 [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_013501.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      D6VZ32, P32794
      UniProtKB/TrEMBL
      A7A1S2, C8ZE06, G2WJL2, N1NZT6
      Conserved Domains (1) summary
      TIGR01243
      Location:51 → 773
      CDC48; AAA family ATPase, CDC48 subfamily
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P32794.1

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