DIS3 exosome catalytic subunit DIS3 [Saccharomyces cerevisiae S288C] - Gene

Summary

Go to the top of the page Help

Official Symbol: DIS3
Official Full Name: exosome catalytic subunit DIS3
Primary source: SGD:S000005381
Locus tag: YOL021C
See related: AllianceGenome:SGD:S000005381; FungiDB:YOL021C; VEuPathDB:YOL021C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: MTR17; RRP44
Summary: Enables 3'-5'-RNA exonuclease activity; RNA endonuclease activity; and tRNA binding activity. Involved in RNA metabolic process. Located in nucleolus. Part of cytoplasmic exosome (RNase complex) and nuclear exosome (RNase complex). Used to study multiple myeloma. Human ortholog(s) of this gene implicated in multiple myeloma. Orthologous to human DIS3 (DIS3 homolog, exosome endoribonuclease and 3'-5' exoribonuclease). [provided by Alliance of Genome Resources, Dec 2024]
NEW: Try the new Gene table
Try the new Transcript table

Genomic context

Go to the top of the page Help

See DIS3 in Genome Data Viewer

Location:: chromosome: XV

Exon count:: 1

Sequence:: Chromosome: XV; NC_001147.6 (282421..285426, complement)

Chromosome XV - NC_001147.6 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

Go to the top of the page Help

Go to reference sequence details

Genomic Sequence:: NC_001147.6

Go to nucleotide: Graphics FASTA GenBank

Bibliography

Go to the top of the page Help

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The DIS3 mutations do not increase DNA damage or RNA processing defects that lead to the accumulation of polyadenylated RNA in the nucleus and specific DIS3 activities support mitotic fidelity in yeast.
Title: Exonuclease domain mutants of yeast DIS3 display genome instability.
3' phosphate RNA is not a substrate for Rrp6 but is readily degraded by Rrp44 in the nuclear exosome.
Title: Nuclear RNA Exosome at 3.1 Å Reveals Substrate Specificities, RNA Paths, and Allosteric Inhibition of Rrp44/Dis3.
Rrp41 mutations blocked substrate passage through the channel to Rrp44 only for cytoplasmic mRNAs, supporting the predicted widening of the lumen in the Rrp6-associated.Many exosome substrates exhibited clear preferences for a specific path to Rrp44. Both threading and direct access routes involve the RNA helicase Mtr4
Title: Transcriptome-wide analysis of alternative routes for RNA substrates into the exosome complex.
While the molecular functions of Rrp44 and the RNA exosome have been extensively characterized, our studies characterize how this molecular function affects the physiology of the organism.
Title: The RNA exosome affects iron response and sensitivity to oxidative stress.
Rrp41 mutation caused accumulation of nuclear and cytoplasmic exosome substrates including the non-stop decay reporter, for which degradation is dependent on either endonucleolytic or exonucleolytic Dis3 activities.
Title: The RNA exosome complex central channel controls both exonuclease and endonuclease Dis3 activities in vivo and in vitro.
Data shows that Dis3p and Rrp6p from S. cerevisiae strain W303 have both overlapping and specific roles in degrading distinct classes of RNA substrates.
Title: Extensive degradation of RNA precursors by the exosome in wild-type cells.
Data indicate that unspliced pre-mRNAs were also identified as targets for Rrp44 and Rrp6.
Title: Transcriptome-wide analysis of exosome targets.
instead of unwinding RNA in single base pair steps, Rrp44 accumulates the energy released by multiple single nucleotide step hydrolysis reactions until about four base pairs are unwound in a burst
Title: Elastic coupling between RNA degradation and unwinding by an exoribonuclease.
DIS3 mutant showed extensive microtubule defects.
Title: Pronounced and extensive microtubule defects in a Saccharomyces cerevisiae DIS3 mutant.
The N-terminal PIN domain of Rrp44 was shown to be necessary and sufficient for association with the core exosome, indicating its dual function as a nuclease and structural element.
Title: The N-terminal PIN domain of the exosome subunit Rrp44 harbors endonuclease activity and tethers Rrp44 to the yeast core exosome.

Submit: New GeneRIF Correction See all GeneRIFs (14)

Pathways from PubChem

Go to the top of the page

Interactions

Go to the top of the page Help

Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

Go to the top of the page Help

Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables 3'-5'-RNA exonuclease activity	IBA Inferred from Biological aspect of Ancestor more info
enables 3'-5'-RNA exonuclease activity	IDA Inferred from Direct Assay more info	PubMed
enables 3'-5'-RNA exonuclease activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables RNA binding	IEA Inferred from Electronic Annotation more info
enables RNA endonuclease activity	IDA Inferred from Direct Assay more info	PubMed
enables RNA endonuclease activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables RNA nuclease activity	IEA Inferred from Electronic Annotation more info
enables endonuclease activity	IBA Inferred from Biological aspect of Ancestor more info
enables endonuclease activity	IEA Inferred from Electronic Annotation more info
enables exonuclease activity	IEA Inferred from Electronic Annotation more info
enables tRNA binding	IDA Inferred from Direct Assay more info	PubMed
enables tRNA binding	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
involved_in TRAMP-dependent tRNA surveillance pathway	IDA Inferred from Direct Assay more info	PubMed
involved_in TRAMP-dependent tRNA surveillance pathway	IGI Inferred from Genetic Interaction more info	PubMed
involved_in TRAMP-dependent tRNA surveillance pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mRNA processing	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nonfunctional rRNA decay	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nuclear mRNA surveillance	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nuclear mRNA surveillance of mRNA 3'-end processing	IBA Inferred from Biological aspect of Ancestor more info
involved_in nuclear polyadenylation-dependent CUT catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nuclear polyadenylation-dependent rRNA catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in rRNA catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in rRNA catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in rRNA processing	IEA Inferred from Electronic Annotation more info
involved_in regulatory ncRNA 3'-end processing	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
part_of cytoplasmic exosome (RNase complex)	IBA Inferred from Biological aspect of Ancestor more info
part_of cytoplasmic exosome (RNase complex)	IDA Inferred from Direct Assay more info	PubMed
part_of exosome (RNase complex)	IEA Inferred from Electronic Annotation more info
located_in mitochondrion	HDA more info	PubMed
located_in mitochondrion	IEA Inferred from Electronic Annotation more info
part_of nuclear exosome (RNase complex)	IBA Inferred from Biological aspect of Ancestor more info
part_of nuclear exosome (RNase complex)	IDA Inferred from Direct Assay more info	PubMed
located_in nucleolus	HDA more info	PubMed
located_in nucleolus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleolus	IEA Inferred from Electronic Annotation more info
located_in nucleus	HDA more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

Go to the top of the page Help

Preferred Names: exosome catalytic subunit DIS3

NP_014621.1

Exosome core complex catalytic subunit; has both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; role in degradation of tRNAs; similar to E. coli RNase R and to human DIS3, which partially complements dis3-81 heat sensitivity; mutations in Dis3p analogous to human mutations implicated in multiple myeloma impair exosome function; protein abundance increases under to DNA replication stress

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001147.6 Reference assembly

Range

282421..285426 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001183275.1 → NP_014621.1 TPA: exosome catalytic subunit DIS3 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_014621.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W244, Q08162

UniProtKB/TrEMBL

C7GVF0, G2WML0, N1NY46

Conserved Domains (3) summary

COG0557
Location:281 → 989

VacB; Exoribonuclease R [Transcription]

cd09862
Location:25 → 221

PIN_Rrp44-like; VapC-like PIN domain of yeast exosome subunit Rrp44 endoribonuclease and other eukaryotic homologs

pfam17216
Location:252 → 399

Rrp44_CSD1; Rrp44-like cold shock domain