RAD52 recombinase RAD52 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: RAD52
Official Full Name: recombinase RAD52
Primary source: SGD:S000004494
Locus tag: YML032C
See related: AllianceGenome:SGD:S000004494; FungiDB:YML032C; VEuPathDB:YML032C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables DNA strand exchange activity and DNA/DNA annealing activity. Involved in DNA metabolic process. Located in nuclear chromosome. Human ortholog(s) of this gene implicated in gastrointestinal system cancer (multiple); lung cancer (multiple); mucoepidermoid carcinoma; and nicotine dependence. Orthologous to human RAD52 (RAD52 homolog, DNA repair protein). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See RAD52 in Genome Data Viewer

Location:: chromosome: XIII

Exon count:: 1

Sequence:: Chromosome: XIII; NC_001145.3 (212515..213930, complement)

Chromosome XIII - NC_001145.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001145.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Yeast Rad52 is a homodecamer and possesses BRCA2-like bipartite Rad51 binding modes.
Title: Yeast Rad52 is a homodecamer and possesses BRCA2-like bipartite Rad51 binding modes.
RAD52 influences the effect of BRCA1/2 missense variants on homologous recombination and gene reversion in Saccharomyces cerevisiae.
Title: RAD52 influences the effect of BRCA1/2 missense variants on homologous recombination and gene reversion in Saccharomyces cerevisiae.
Replication Protein A Phosphorylation Facilitates RAD52-Dependent Homologous Recombination in BRCA-Deficient Cells.
Title: Replication Protein A Phosphorylation Facilitates RAD52-Dependent Homologous Recombination in BRCA-Deficient Cells.
Single molecule microscopy reveals key physical features of repair foci in living cells.
Title: Single molecule microscopy reveals key physical features of repair foci in living cells.
Emerging non-canonical roles for the Rad51-Rad52 interaction in response to double-strand breaks in yeast.
Title: Emerging non-canonical roles for the Rad51-Rad52 interaction in response to double-strand breaks in yeast.
Cooperation between Rad52 liquid droplets and various types of nuclear filaments promotes the assembly and function of the DNA repair centre.
Title: DNA repair by Rad52 liquid droplets.
In S. cerevisiae telomere addition is significantly reduced in the absence of the Rad51 recombinase, while loss of Rad52, required for Rad51 nucleoprotein filament formation, has no effect. Deletion of RAD52 suppresses the defect of the rad51Delta strain, suggesting that Rad52 inhibits de novo telomere addition in the absence of Rad51.
Title: Interaction of yeast Rad51 and Rad52 relieves Rad52-mediated inhibition of de novo telomere addition.
Rad52 competes with Sgs1 for DNA end binding and inhibits Sgs1 translocation along DNA, identifying a role for Rad52 in limiting ssDNA generated by end resection.
Title: Rad52 Restrains Resection at DNA Double-Strand Break Ends in Yeast.
we observed a similar increase in repeat instability in RNase H-deficient cells when we either changed the direction of transcription-replication collisions, or flipped the repeat sequence such that the (UUC)n run occurred in the transcript. The increase in repeat expansions in RNase H-deficient strains was dependent on Rad52 and Pol32 proteins, suggesting that break-induced replication (BIR) is responsible for this effe
Title: RNA-DNA hybrids promote the expansion of Friedreich's ataxia (GAA)n repeats via break-induced replication.
Rad52-RFP and Rad51-GFP co-localize to the same DSB, but a significant minority of foci have Rad51-GFP without visible Rad52-RFP. We conclude that co-localization of foci in cells with 3 DSBs does not represent formation of a homologous recombination "repair center," as the same distribution of Ddc2-GFP foci was found in the absence of the Rad52 protein.
Title: Live cell monitoring of double strand breaks in S. cerevisiae.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables DNA strand exchange activity	IDA Inferred from Direct Assay more info	PubMed
enables DNA/DNA annealing activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA amplification	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in DNA recombinase assembly	IDA Inferred from Direct Assay more info	PubMed
involved_in DNA recombinase assembly	IEA Inferred from Electronic Annotation more info
involved_in DNA recombination	IEA Inferred from Electronic Annotation more info
involved_in DNA repair	IEA Inferred from Electronic Annotation more info
involved_in double-strand break repair	IEA Inferred from Electronic Annotation more info
involved_in double-strand break repair via break-induced replication	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in double-strand break repair via homologous recombination	IBA Inferred from Biological aspect of Ancestor more info
involved_in double-strand break repair via homologous recombination	IEA Inferred from Electronic Annotation more info
involved_in double-strand break repair via homologous recombination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in double-strand break repair via single-strand annealing	IBA Inferred from Biological aspect of Ancestor more info
involved_in double-strand break repair via single-strand annealing	IEA Inferred from Electronic Annotation more info
involved_in double-strand break repair via single-strand annealing	IGI Inferred from Genetic Interaction more info	PubMed
involved_in meiotic joint molecule formation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in meiotic joint molecule formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitochondrial DNA repair	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic recombination	IBA Inferred from Biological aspect of Ancestor more info
involved_in postreplication repair	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in telomere maintenance via recombination	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in mitochondrion	IEA Inferred from Electronic Annotation more info
located_in nuclear chromosome	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	HDA more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: recombinase RAD52

NP_013680.2

Protein involved in homologous recombination; attenuates resection of DNA double-strand break ends and stimulates strand exchange by facilitating Rad51p binding to single-stranded DNA (ssDNA); anneals complementary ssDNA; involved in the repair of double-strand breaks in DNA during vegetative growth, meiosis and UV induced sister chromatid recombination; forms a homodecameric ring; phosphorylation by Cdc28p in G2/M phase promotes Rad52p ring interaction that leads to superstructure formation

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001145.3 Reference assembly

Range

212515..213930 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001182390.1 → NP_013680.2 TPA: recombinase RAD52 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_013680.2

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VZE2, P06778

UniProtKB/TrEMBL

B3LLM0, C7GT62

Conserved Domains (1) summary

cl01936
Location:1 → 382

Rad52_Rad22; Rad52/22 family double-strand break repair protein