SGT2 Sgt2p [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: SGT2
Official Full Name: Sgt2p
Primary source: SGD:S000005533
Locus tag: YOR007C
See related: AllianceGenome:SGD:S000005533; FungiDB:YOR007C; VEuPathDB:YOR007C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables molecular adaptor activity. Involved in post-translational protein targeting to endoplasmic reticulum membrane and response to heat. Located in cytoplasm. Part of TRC complex. Orthologous to human SGTA (small glutamine rich tetratricopeptide repeat co-chaperone alpha) and SGTB (small glutamine rich tetratricopeptide repeat co-chaperone beta). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See SGT2 in Genome Data Viewer

Location:: chromosome: XV

Exon count:: 1

Sequence:: Chromosome: XV; NC_001147.6 (338938..339978, complement)

Chromosome XV - NC_001147.6 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001147.6

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Molecular basis of tail-anchored integral membrane protein recognition by the cochaperone Sgt2.
Title: Molecular basis of tail-anchored integral membrane protein recognition by the cochaperone Sgt2.
Ribosome-bound Get4/5 facilitates the capture of tail-anchored proteins by Sgt2 in yeast.
Title: Ribosome-bound Get4/5 facilitates the capture of tail-anchored proteins by Sgt2 in yeast.
Report demonstrated that Ybr137wp and the Sgt2 TPR domain interact directly.
Title: Structural and functional characterization of ybr137wp implicates its involvement in the targeting of tail-anchored proteins to membranes.
SGTA(Sgt2) associates with and specifically regulates the androgen, glucocorticoid, and progesterone receptors and has no effect on the mineralocorticoid and estrogen receptors in both yeast and mammalian cell-based reporter assays.
Title: The cochaperone SGTA (small glutamine-rich tetratricopeptide repeat-containing protein alpha) demonstrates regulatory specificity for the androgen, glucocorticoid, and progesterone receptors.
The crystal structure of Sgt2 dimerization domain complexed with the Get5 Ubl domain (Sgt2N-Get5Ubl) is reported.
Title: Structure of the Sgt2 dimerization domain complexed with the Get5 UBL domain involved in the targeting of tail-anchored membrane proteins to the endoplasmic reticulum.
Structures of the Sgt2/SGTA dimerization domain with the Get5/UBL4A UBL domain reveal an interaction that forms a conserved dynamic interface.
Title: Structures of the Sgt2/SGTA dimerization domain with the Get5/UBL4A UBL domain reveal an interaction that forms a conserved dynamic interface.
solve a structure of the Sgt2_NT/Get5_UBL complex, validate it via site-directed mutagenesis, and empirically determine its stoichiometry using relaxation experiments and isothermal titration calorimetry
Title: Structure of the Sgt2/Get5 complex provides insights into GET-mediated targeting of tail-anchored membrane proteins.
Sgt2 increases the ability of excess Ssa to counteract [PSI(+)] curing by excess Hsp104. One region of Sgt2 interacts both with the prion domain of Sup35 and with tail-anchored proteins.
Title: Regulation of chaperone effects on a yeast prion by cochaperone Sgt2.
a structural model of the Sgt2-Get4/Get5-HSC complex.
Title: A structural model of the Sgt2 protein and its interactions with chaperones and the Get4/Get5 complex.
S. cerevisiae sgt2Delta cells show partial defects in TA protein sorting.
Title: Cooperative and independent activities of Sgt2 and Get5 in the targeting of tail-anchored proteins.

Submit: New GeneRIF Correction See all GeneRIFs (12)

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables molecular adaptor activity	IBA Inferred from Biological aspect of Ancestor more info
enables molecular adaptor activity	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
involved_in post-translational protein targeting to endoplasmic reticulum membrane	IBA Inferred from Biological aspect of Ancestor more info
involved_in post-translational protein targeting to endoplasmic reticulum membrane	IDA Inferred from Direct Assay more info	PubMed
involved_in response to heat	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of TRC complex	IBA Inferred from Biological aspect of Ancestor more info
part_of TRC complex	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	HDA more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
is_active_in membrane	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: Sgt2p

NP_014649.1

Glutamine-rich cytoplasmic cochaperone; serves as a scaffold bringing together Get4, Get5p, and other TRC complex members that are required to mediate posttranslational insertion of tail-anchored proteins into the ER membrane; interacts with the prion domain of Sup35p; amyloid sensor; plays a role in targeting chaperones to prion aggregates; similar to human cochaperone SGT; forms cytoplasmic foci upon DNA replication stress

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001147.6 Reference assembly

Range

338938..339978 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001183426.1 → NP_014649.1 TPA: Sgt2p [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_014649.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W273, Q12118

UniProtKB/TrEMBL

A6ZNL2, B3LJ62, C7GJU6, C8ZHX0, G2WMN7, N1NY70

Conserved Domains (4) summary

smart00727
Location:264 → 298

STI1; Heat shock chaperonin-binding motif

PLN03088
Location:102 → 240

PLN03088; SGT1, suppressor of G2 allele of SKP1; Provisional

sd00006
Location:102 → 130

TPR; TPR repeat [structural motif]

pfam16546
Location:7 → 71

SGTA_dimer; Homodimerization domain of SGTA