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    ENT5 Ent5p [ Saccharomyces cerevisiae S288C ]

    Gene ID: 851731, updated on 9-Dec-2024

    Summary

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    Official Symbol
    ENT5
    Official Full Name
    Ent5p
    Primary source
    SGD:S000002560
    Locus tag
    YDR153C
    See related
    AllianceGenome:SGD:S000002560; FungiDB:YDR153C; VEuPathDB:YDR153C
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Summary
    Enables clathrin binding activity and phosphatidylinositol-3,5-bisphosphate binding activity. Involved in Golgi to endosome transport; early endosome to Golgi transport; and late endosome to vacuole transport via multivesicular body sorting pathway. Located in endosome. Part of clathrin vesicle coat. [provided by Alliance of Genome Resources, Dec 2024]
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    Genomic context

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    See ENT5 in Genome Data Viewer
    Location:
    chromosome: IV
    Exon count:
    1
    Sequence:
    Chromosome: IV; NC_001136.10 (766736..767971, complement)

    Chromosome IV - NC_001136.10Genomic Context describing neighboring genes Neighboring gene putative mRNA-binding protein CTH1 Neighboring gene Gir2p Neighboring gene peptidylprolyl isomerase CPR1 Neighboring gene DNA-directed RNA polymerase I subunit RPA14 Neighboring gene uncharacterized protein

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_001136.10

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Structural and functional insight into the N-terminal domain of the clathrin adaptor Ent5 from Saccharomyces cerevisiae. Zhang F, et al. Biochem Biophys Res Commun, 2016 Sep 2. PMID 27369074
    2. Structural basis for the specificity of the GAE domain of yGGA2 for its accessory proteins Ent3 and Ent5 . Fang P, et al. Biochemistry, 2010 Sep 14. PMID 20704189
    3. Distinct functional domains of the epsin-related Ent5p, a cargo adaptor for the SNARE Tlg2p in transport between endosomes and Golgi. Petersen L, et al. Traffic, 2023 Oct. PMID 37434343
    4. Ent5p is required with Ent3p and Vps27p for ubiquitin-dependent protein sorting into the multivesicular body. Eugster A, et al. Mol Biol Cell, 2004 Jul. PMID 15107463, Free PMC Article
    5. Adaptor autoregulation promotes coordinated binding within clathrin coats. Hung CW, et al. J Biol Chem, 2012 May 18. PMID 22457357, Free PMC Article

    See all (62) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Above results facilitate the understanding of the ScEnt5-mediated vesicle coat formation process.
      Title: Structural and functional insight into the N-terminal domain of the clathrin adaptor Ent5 from Saccharomyces cerevisiae.
    2. Ent5 clathrin binding performs a mechanistic role in clathrin coat maturation, whereas Ent5 adaptor binding promotes cargo incorporation.
      Title: Clathrin binding by the adaptor Ent5 promotes late stages of clathrin coat maturation.
    3. Here we show not all acidic-phenylalanine motifs in Ent5 are active for yGGA2_GAE domain binding
      Title: Structural basis for the specificity of the GAE domain of yGGA2 for its accessory proteins Ent3 and Ent5 .
    4. Ent5p is a connector between PtdIns(3,5)P(2)- and the Vps27p-ubiquitin-driven sorting machinery at the multivesicular body.
      Title: Ent5p is required with Ent3p and Vps27p for ubiquitin-dependent protein sorting into the multivesicular body.
    5. the clathrin adaptors AP-1, Ent3p, Ent5p, and the Ggas cooperate in different ways to sort proteins between the TGN and the endosomes
      Title: Ent3p and Ent5p exhibit cargo-specific functions in trafficking proteins between the trans-Golgi network and the endosomes in yeast.
    6. These results differentiate Ent3p and Ent5p function in vivo, suggesting that Ent3p acts primarily with Gga proteins, whereas Ent5p acts with both AP-1 and Gga proteins but is more critical for AP-1-mediated transport.
      Title: Distinct roles for TGN/endosome epsin-like adaptors Ent3p and Ent5p.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    enables clathrin binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables clathrin binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables lipid binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables phosphatidylinositol-3,5-bisphosphate binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables phosphatidylinositol-3,5-bisphosphate binding IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables phospholipid binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    Process Evidence Code Pubs
    involved_in Golgi to endosome transport IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in actin filament organization IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in early endosome to Golgi transport IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in endocytosis IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in late endosome to vacuole transport via multivesicular body sorting pathway IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in protein transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    part_of clathrin vesicle coat IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of clathrin vesicle coat IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cytoplasm HDA PubMed 
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cytosol IEA
    Inferred from Electronic Annotation
    more info
    		  
    is_active_in endosome IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in endosome IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in endosome IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in endosome membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    is_active_in plasma membrane IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  

    General protein information

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    Preferred Names
    Ent5p
    NP_010437.3
    • Protein containing an N-terminal epsin-like domain; involved in clathrin recruitment and traffic between the Golgi and endosomes; associates with the clathrin adaptor Gga2p, clathrin adaptor complex AP-1, and clathrin

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001136.10 Reference assembly

      Range
      766736..767971 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001180460.3 → NP_010437.3  TPA: Ent5p [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_010437.3

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      D6VSD5, Q03769, Q6Q5U2
      UniProtKB/TrEMBL
      C7GX35
      Conserved Domains (1) summary
      cd16993
      Location:33 → 190
      ENTH_Ent5; Epsin N-Terminal Homology (ENTH) domain of Yeast Ent5 and similar proteins
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q03769.1

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