RAD23 Rad23p [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: RAD23
Official Full Name: Rad23p
Primary source: SGD:S000000763
Locus tag: YEL037C
See related: AllianceGenome:SGD:S000000763; FungiDB:YEL037C; VEuPathDB:YEL037C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables several functions, including K48-linked polyubiquitin modification-dependent protein binding activity; proteasome binding activity; and ubiquitin binding activity. Contributes to peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase activity. Involved in negative regulation of transcription by RNA polymerase II; proteasomal protein catabolic process; and protein deglycosylation. Located in mitochondrion. Part of nucleotide-excision repair factor 2 complex. Orthologous to several human genes including RAD23A (RAD23 homolog A, nucleotide excision repair protein). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See RAD23 in Genome Data Viewer

Location:: chromosome: V

Exon count:: 1

Sequence:: Chromosome: V; NC_001137.3 (81407..82603, complement)

Chromosome V - NC_001137.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001137.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Exceptional origin activation revealed by comparative analysis in two laboratory yeast strains.
Title: Exceptional origin activation revealed by comparative analysis in two laboratory yeast strains.
Tethering-facilitated DNA 'opening' and complementary roles of beta-hairpin motifs in the Rad4/XPC DNA damage sensor protein.
Title: Tethering-facilitated DNA 'opening' and complementary roles of β-hairpin motifs in the Rad4/XPC DNA damage sensor protein.
Ubp12 removes short ubiquitin chains from the N-terminal ubiquitin-like domain (UbL) of Rad23.
Title: The deubiquitylating enzyme Ubp12 regulates Rad23-dependent proteasomal degradation.
The structures of proteasome substrate receptor complexes with the shuttle factors that deliver ubiquitinated proteins to proteasomes have been solved, namely human Rpn13 complexed with PLIC2 and Saccharomyces cerevisiae Rpn1 with Rad23.
Title: Structures of Rpn1 T1:Rad23 and hRpn13:hPLIC2 Reveal Distinct Binding Mechanisms between Substrate Receptors and Shuttle Factors of the Proteasome.
Rad4-Rad23 forms non-motile complexes or conducts a one-dimensional search for DNA lesions via either random diffusion or constrained motion.
Title: Single-Molecule Imaging Reveals that Rad4 Employs a Dynamic DNA Damage Recognition Process.
Identify the Cdc48-Rad23/Dsk2 axis as a major route to the proteasome.
Title: In Vivo Ubiquitin Linkage-type Analysis Reveals that the Cdc48-Rad23/Dsk2 Axis Contributes to K48-Linked Chain Specificity of the Proteasome.
these studies are consistent with a model in which Rad23p partners with the deglycosylating enzyme Png1p to reduce manganese toxicity through proteosomal degradation of glycosylated substrate(s).
Title: Phosphate disruption and metal toxicity in Saccharomyces cerevisiae: effects of RAD23 and the histone chaperone HPC2.
The action of Cdc48 could free Rad23 from Ufd2 to allow its subsequent association with Rpn1, which in turn may facilitate the orderly transfer of the substrate from the ubiquitylation apparatus to the proteasome.
Title: The Cdc48 ATPase modulates the interaction between two proteolytic factors Ufd2 and Rad23.
Rad23 escapes degradation because it lacks a proteasome initiation region.
Title: Rad23 escapes degradation because it lacks a proteasome initiation region.
C-terminal UBA domain of Rad23 ubiquitin receptor protects it by preventing initiation of protein degradation at the proteasome.
Title: C-terminal UBA domains protect ubiquitin receptors by preventing initiation of protein degradation.

Submit: New GeneRIF Correction See all GeneRIFs (25)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables K48-linked polyubiquitin modification-dependent protein binding	IDA Inferred from Direct Assay more info	PubMed
enables damaged DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables damaged DNA binding	IEA Inferred from Electronic Annotation more info
contributes_to peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase activity	IDA Inferred from Direct Assay more info	PubMed
enables polyubiquitin modification-dependent protein binding	IBA Inferred from Biological aspect of Ancestor more info
enables proteasome binding	IBA Inferred from Biological aspect of Ancestor more info
enables proteasome binding	IDA Inferred from Direct Assay more info	PubMed
enables protein-macromolecule adaptor activity	IPI Inferred from Physical Interaction more info	PubMed
enables ubiquitin binding	IBA Inferred from Biological aspect of Ancestor more info
enables ubiquitin binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA repair	IEA Inferred from Electronic Annotation more info
involved_in ERAD pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nucleotide-excision repair	IEA Inferred from Electronic Annotation more info
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process	IEA Inferred from Electronic Annotation more info
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein deglycosylation	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
located_in mitochondrion	HDA more info	PubMed
is_active_in nucleoplasm	IBA Inferred from Biological aspect of Ancestor more info
part_of nucleotide-excision repair factor 2 complex	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: Rad23p

NP_010877.3

Proteasome-associated ubiquitin receptor; K48-specific Ub chain binding protein that recruits substrates to the proteasome; subunit of nuclear excision repair factor 2 (NER2) with Rad4p that binds damaged DNA during NER, linking repair to proteolysis; NER2 binds DDR gene promoters to repress transcription in the absence of damage; stimulates Png1p-induced protein deglycosylation; contains a Ub-like (UBL) and two Ub-associated (UBA) domains; UBA2 protects Rad23p from proteasomal degradation