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    ROT2 glucan 1,3-alpha-glucosidase ROT2 [ Saccharomyces cerevisiae S288C ]

    Gene ID: 852530, updated on 9-Dec-2024

    Summary

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    Official Symbol
    ROT2
    Official Full Name
    glucan 1,3-alpha-glucosidase ROT2
    Primary source
    SGD:S000000433
    Locus tag
    YBR229C
    See related
    AllianceGenome:SGD:S000000433; FungiDB:YBR229C; VEuPathDB:YBR229C
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Also known as
    GLS2
    Summary
    Enables Glc2Man9GlcNAc2 oligosaccharide glucosidase activity and glucan 1,3-alpha-glucosidase activity. Involved in N-glycan processing and fungal-type cell wall beta-glucan biosynthetic process. Located in endoplasmic reticulum lumen. Part of glucosidase II complex. Human ortholog(s) of this gene implicated in polycystic kidney disease; polycystic kidney disease 3; and polycystic liver disease. Orthologous to human GANAB (glucosidase II alpha subunit) and GANC (glucosidase alpha, neutral C). [provided by Alliance of Genome Resources, Dec 2024]
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    Genomic context

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    See ROT2 in Genome Data Viewer
    Location:
    chromosome: II
    Exon count:
    1
    Sequence:
    Chromosome: II; NC_001134.8 (676357..679221, complement)

    Chromosome II - NC_001134.8Genomic Context describing neighboring genes Neighboring gene Mcx1p Neighboring gene endonuclease Neighboring gene Om14p Neighboring gene Coq21p

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_001134.8

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Isolation and functional characterization of Sporothrix schenckii ROT2, the encoding gene for the endoplasmic reticulum glucosidase II. Robledo-Ortiz CI, et al. Fungal Biol, 2012 Aug. PMID 22862919
    2. Mechanism-based inhibition of yeast alpha-glucosidase and human pancreatic alpha-amylase by a new class of inhibitors. 2-Deoxy-2,2-difluoro-alpha-glycosides. Braun C, et al. J Biol Chem, 1995 Nov 10. PMID 7592915
    3. N-linked oligosaccharide processing enzyme glucosidase II produces 1,5-anhydrofructose as a side product. Hirano K, et al. Glycobiology, 2000 Dec. PMID 11159920
    4. Comparative study of the inhibition of alpha-glucosidase, alpha-amylase, and cyclomaltodextrin glucanosyltransferase by acarbose, isoacarbose, and acarviosine-glucose. Kim MJ, et al. Arch Biochem Biophys, 1999 Nov 15. PMID 10545215
    5. Yeast GTB1 encodes a subunit of glucosidase II required for glycoprotein processing in the endoplasmic reticulum. Wilkinson BM, et al. J Biol Chem, 2006 Mar 10. PMID 16373354

    See all (69) citations in PubMed

    GeneRIFs: Gene References Into Functions

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    enables Glc2Man9GlcNAc2 oligosaccharide glucosidase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables Glc2Man9GlcNAc2 oligosaccharide glucosidase activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables alpha-glucosidase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables carbohydrate binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables catalytic activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables glucan 1,3-alpha-glucosidase activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables hydrolase activity, acting on glycosyl bonds IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables hydrolase activity, hydrolyzing O-glycosyl compounds IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in N-glycan processing IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in N-glycan processing IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in carbohydrate metabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in fungal-type cell wall beta-glucan biosynthetic process IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in endoplasmic reticulum HDA PubMed 
    located_in endoplasmic reticulum IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in endoplasmic reticulum lumen IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of glucosidase II complex IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of glucosidase II complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    located_in mitochondrion HDA PubMed 

    General protein information

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    Preferred Names
    glucan 1,3-alpha-glucosidase ROT2
    NP_009788.3
    • Glucosidase II catalytic subunit; required to trim the final glucose in N-linked glycans; required for normal cell wall synthesis; mutations in rot2 suppress tor2 mutations, and are synthetically lethal with rot1 mutations

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001134.8 Reference assembly

      Range
      676357..679221 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001178577.3 → NP_009788.3  TPA: glucan 1,3-alpha-glucosidase ROT2 [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_009788.3

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      P38138
      UniProtKB/TrEMBL
      A6ZLG7
      Conserved Domains (2) summary
      cd06603
      Location:380 → 850
      GH31_GANC_GANAB_alpha; neutral alpha-glucosidase C, neutral alpha-glucosidase AB
      cd14752
      Location:259 → 380
      GH31_N; N-terminal domain of glycosyl hydrolase family 31 (GH31)
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P38138.1

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