mid1 anillin-related medial ring protein Mid1 [Schizosaccharomyces pombe (fission yeast)] - Gene

Summary

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Official Symbol: mid1
Official Full Name: anillin-related medial ring protein Mid1
Primary source: PomBase:SPCC4B3.15
Locus tag: SPOM_SPCC4B3.15
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Schizosaccharomyces pombe (strain: 972h-)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina; Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces
Also known as: dmf1
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Genomic context

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See mid1 in Genome Data Viewer

Location:: chromosome: III

Exon count:: 1

Sequence:: Chromosome: III; NC_003421.2 (1147316..1150435, complement)

Chromosome III - NC_003421.2 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003421.2

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Fission yeast Pak1 phosphorylates anillin-like Mid1 for spatial control of cytokinesis.
Title: Fission yeast Pak1 phosphorylates anillin-like Mid1 for spatial control of cytokinesis.
The C-terminal half of Mid1p consists of lipid binding C2 and PH domains, but the N-terminal half (Mid1p-N452) performs most of the functions of the full-length protein. Mid1p-N452 is intrinsically disordered but moderately compact. Recombinant Mid1p-N452 purified from insect cells was phosphorylated, which weakens its tendency to aggregate.
Title: The Functionally Important N-Terminal Half of Fission Yeast Mid1p Anillin Is Intrinsically Disordered and Undergoes Phase Separation.
These findings identify Mid1 as a key Sid2 substrate for SIN-mediated remodeling of the division site for efficient cytokinesis and provide evidence that nodes serve to integrate signals coordinating cell cycle progression and cytokinesis.
Title: NDR Kinase Sid2 Drives Anillin-like Mid1 from the Membrane to Promote Cytokinesis and Medial Division Site Placement.
Unlike in wild-type cells, actomyosin rings in cells rewired to divide medially in the absence of Mid1p assemble late in anaphase. The rewiring process affects the ability of the actomyosin ring to track the nucleus upon perturbation of nuclear position.
Title: Rewiring Mid1p-independent medial division in fission yeast.
Pom1 inhibits Cdr2 interaction with Mid1, reducing its clustering ability.
Title: Pom1 regulates the assembly of Cdr2-Mid1 cortical nodes for robust spatial control of cytokinesis.
An overview of the studies that have deciphered how Mid1 localization and scaffolding activities are controlled over the cell cycle to ensure the symmetrical division of fission yeast cells, is provided.
Title: Mid1/anillin and the spatial regulation of cytokinesis in fission yeast.
Orderly assembly of the contractile ring in wild-type cells depends on Mid1p.
Title: Anillin-related protein Mid1p coordinates the assembly of the cytokinetic contractile ring in fission yeast.
Residues 1-578 of Mid1p assemble node components myosin-II, Rng2p, and Cdc15p, and the insoluble domain facilitates the normal, efficient condensation of nodes into rings.
Title: Characterization of structural and functional domains of the anillin-related protein Mid1p that contribute to cytokinesis in fission yeast.
Blt1 acts as a scaffolding protein for precursors of the cytokinetic ring, and Blt1 and Mid1 provide overlapping membrane anchors for proper division plane positioning.
Title: Blt1 and Mid1 provide overlapping membrane anchors to position the division plane in fission yeast.
The N-terminus of Mid1 physically interacts with cytokinesis node proteins.
Title: Characterization of Mid1 domains for targeting and scaffolding in fission yeast cytokinesis.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by PomBase

Function	Evidence Code	Pubs
enables cytoskeletal protein-membrane anchor activity	EXP Inferred from Experiment more info	PubMed
enables cytoskeletal protein-membrane anchor activity	IPI Inferred from Physical Interaction more info	PubMed
enables lipid binding	EXP Inferred from Experiment more info	PubMed
enables phosphatidylinositol-4,5-bisphosphate binding	IDA Inferred from Direct Assay more info	PubMed
enables polo box domain specific binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein-membrane adaptor activity	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
involved_in actomyosin contractile ring assembly	IBA Inferred from Biological aspect of Ancestor more info
involved_in mitotic actomyosin contractile ring assembly	EXP Inferred from Experiment more info	PubMed
involved_in mitotic actomyosin contractile ring assembly	IGI Inferred from Genetic Interaction more info	PubMed
involved_in mitotic actomyosin contractile ring assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic cytokinesis	IBA Inferred from Biological aspect of Ancestor more info
involved_in mitotic cytokinesis, division site positioning	EXP Inferred from Experiment more info	PubMed
involved_in mitotic cytokinesis, division site positioning	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in septin ring organization	IBA Inferred from Biological aspect of Ancestor more info
involved_in septin ring organization	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in actomyosin contractile ring	IBA Inferred from Biological aspect of Ancestor more info
is_active_in medial cortex	IDA Inferred from Direct Assay more info	PubMed
is_active_in medial cortical node	IDA Inferred from Direct Assay more info	PubMed
is_active_in mitotic actomyosin contractile ring	IDA Inferred from Direct Assay more info	PubMed
is_active_in mitotic actomyosin contractile ring, proximal layer	IDA Inferred from Direct Assay more info	PubMed
is_active_in nuclear envelope	HDA more info	PubMed
is_active_in nucleus	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: anillin-related medial ring protein Mid1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003421.2 Reference assembly

Range

1147316..1150435 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001023067.3 → NP_588075.1 anillin-related medial ring protein Mid1 [Schizosaccharomyces pombe]

See identical proteins and their annotated locations for NP_588075.1

Status: PROVISIONAL

UniProtKB/Swiss-Prot

P78953

Conserved Domains (2) summary

smart00233
Location:805 → 898

PH; Pleckstrin homology domain

pfam00169
Location:805 → 898

PH; PH domain