CKI1 bifunctional choline kinase/ethanolamine kinase CKI1 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: CKI1
Official Full Name: bifunctional choline kinase/ethanolamine kinase CKI1
Primary source: SGD:S000004123
Locus tag: YLR133W
See related: AllianceGenome:SGD:S000004123; FungiDB:YLR133W; VEuPathDB:YLR133W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables choline kinase activity and ethanolamine kinase activity. Involved in phosphatidylcholine biosynthetic process and phosphatidylethanolamine biosynthetic process. Located in cytoplasm. Used to study epilepsy and microcephaly. Human ortholog(s) of this gene implicated in intellectual disability; megaconial type congenital muscular dystrophy; muscular dystrophy; narcolepsy; and recurrent hypersomnia. Orthologous to several human genes including CHKA (choline kinase alpha) and CHKB (choline kinase beta). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See CKI1 in Genome Data Viewer

Location:: chromosome: XII

Exon count:: 1

Sequence:: Chromosome: XII; NC_001144.5 (408445..410193)

Chromosome XII - NC_001144.5 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001144.5

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Zinc depletion resulted in a concentration-dependent induction of CKI1
Title: Regulation of the Saccharomyces cerevisiae CKI1-encoded choline kinase by zinc depletion.
Ser25 AND Ser30 are major sites of phosphorylation in choline kinase by Protein Kinase C
Title: Phosphorylation of the yeast choline kinase by protein kinase C. Identification of Ser25 and Ser30 as major sites of phosphorylation.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables choline kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables choline kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables choline kinase activity	IEA Inferred from Electronic Annotation more info
enables choline kinase activity	IGI Inferred from Genetic Interaction more info	PubMed
enables choline kinase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables ethanolamine kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables ethanolamine kinase activity	IEA Inferred from Electronic Annotation more info
enables ethanolamine kinase activity	IGI Inferred from Genetic Interaction more info	PubMed
enables ethanolamine kinase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables kinase activity	IEA Inferred from Electronic Annotation more info
enables phosphotransferase activity, alcohol group as acceptor	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in CDP-choline pathway	IEA Inferred from Electronic Annotation more info
involved_in phosphatidylcholine biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in phosphatidylcholine biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in phosphatidylcholine biosynthetic process	IGI Inferred from Genetic Interaction more info	PubMed
involved_in phosphatidylcholine biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in phosphatidylethanolamine biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in phosphatidylethanolamine biosynthetic process	IGI Inferred from Genetic Interaction more info	PubMed
involved_in phosphatidylethanolamine biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in phospholipid biosynthetic process	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in cytoplasm	HDA more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: bifunctional choline kinase/ethanolamine kinase CKI1

NP_013234.1

EC 2.7.1.32

Choline kinase; catalyzes the first step in phosphatidylcholine synthesis via the CDP-choline (Kennedy pathway); exhibits some ethanolamine kinase activity contributing to phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway; CKI1 has a paralog, EKI1, that arose from the whole genome duplication

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001144.5 Reference assembly

Range

408445..410193

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001182020.1 → NP_013234.1 TPA: bifunctional choline kinase/ethanolamine kinase CKI1 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_013234.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VYC8, P20485

UniProtKB/TrEMBL

B3LT94, B5VN61, C7GQC3, N1NY98

Conserved Domains (2) summary

cd05157
Location:148 → 556

ETNK_euk; Euykaryotic Ethanolamine kinase

pfam04428
Location:94 → 144

Choline_kin_N; Choline kinase N-terminus